2. Recombinant Proteins
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His)

HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His)

Cat. No.: HY-P74034
COA
SDS
Handling Instructions Technical Support

Hemagglutinin protein (HA) is a class I viral fusion protein that binds to sialic acid-containing receptors and initiates viral attachment to cells. This binding promotes internalization of virions through various endocytic pathways. HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

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제품 설명

Hemagglutinin protein (HA) is a class I viral fusion protein that binds to sialic acid-containing receptors and initiates viral attachment to cells. This binding promotes internalization of virions through various endocytic pathways. HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background

The Hemagglutinin Protein (HA), a class I viral fusion protein, binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle to the cell. This attachment leads to virion internalization through clathrin-dependent endocytosis or clathrin- and caveolin-independent pathways. HA also plays a crucial role in determining the host range restriction and virulence of the virus. By mediating the fusion of the endocytosed virus particle's membrane with the endosomal membrane, HA enables the penetration of the virus into the cell cytoplasm. In the low pH environment of endosomes, HA2 undergoes an irreversible conformational change, releasing the fusion hydrophobic peptide and forming a competent fusion pore. HA exists as a homotrimer composed of disulfide-linked HA1-HA2 subunits and interacts with human CACNA1C.
Biological Activity

Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.02-0.4 μg/mL for 1% GRBC.
Species

Virus
Source

Sf9 insect cells
Tag

C-His
Accession

P03452 (D18-Q528)
Gene ID

956529  [NCBI]
Molecular Construction
N-term
H1N1 HA (D18-Q528)
Accession # P03452
His
C-term
Protein Length

Extracellular Domain
Synonyms
HA; Hemagglutinin; HA/Hemagglutinin Protein, H1N1 (A/Puerto Rico/8/1934, sf9, His)
AA Sequence

DTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWLLGNPECDPLLPVRSWSYIVETPNSENGICYPGDFIDYEELREQLSSVSSFERFEIFPKESSWPNHNTNGVTAACSHEGKSSFYRNLLWLTEKEGSYPKLKNSYVNKKGKEVLVLWGIHHPPNSKEQQNLYQNENAYVSVVTSNYNRRFTPEIAERPKVRDQAGRMNYYWTLLKPGDTIIFEANGNLIAPMYAFALSRGFGSGIITSNASMHECNTKCQTPLGAINSSLPYQNIHPVTIGECPKYVRSAKLRMVTGLRNIPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNTVIEKMNIQFTAVGKEFNKLEKRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLKNNAKEIGNGCFEFYHKCDNECMESVRNGTYDYPKYSEESKLNREKVDGVKLESMGIYQ

분자량

Approximately 59.2 kDa
Glycosylation
Yes
Purity

≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
선적

Shipping with dry ice.
각종 서류
SDS (251 KB)

COA (221 KB)

Handling Instructions (2659 KB)

HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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상품명:
HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His)
Cat. No.:
HY-P74034
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