2. Recombinant Proteins
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA/Hemagglutinin Protein, H1N1 (sf9, His)

HA/hemagglutinin proteins bind to sialic acid receptors and initiate viral attachment and internalization through clathrin-dependent or -independent pathways. This class I viral fusion protein determines viral host range and virulence. HA/Hemagglutinin Protein, H1N1 (sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

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Descripciòn

HA/hemagglutinin proteins bind to sialic acid receptors and initiate viral attachment and internalization through clathrin-dependent or -independent pathways. This class I viral fusion protein determines viral host range and virulence. HA/Hemagglutinin Protein, H1N1 (sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background

Binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle. This attachment leads to the internalization of the virion through either clathrin-dependent endocytosis or a clathrin- and caveolin-independent pathway. HA/Hemagglutinin Protein plays a crucial role in determining the host range restriction and virulence of the virus. It acts as a Class I viral fusion protein, enabling the penetration of the virus into the cell cytoplasm by mediating the fusion of the endocytosed virus particle's membrane with the endosomal membrane. The acidic pH in endosomes triggers a conformational change in HA2, causing the release of the fusion hydrophobic peptide. Multiple trimers are necessary to form a functional fusion pore.
Species

Virus
Source

Sf9 insect cells
Tag

C-His
Accession

C3W5S1 (M1-Q529)
Gene ID

/
Molecular Construction
N-term
H1N1 HA (M1-Q529)
Accession # C3W5S1
His
C-term
Protein Length

Partial
Synonyms
Hemagglutinin; HA; Influenza A virus (strain swl A/California/04/2009 H1N1)
AA Sequence

MKAILVVLLYTFATANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADTYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPKGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNIPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREEIDGVKLESTRIYQ

Peso molecular

Approximately 65-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Pureza

≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder
Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, pH 7.4, 300 mM NaCl, 5% mannitol, 5% trehalose.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Envío

Room temperature in continental US; may vary elsewhere.
Documentación
SDS (251 KB)

COA (221 KB)

Instrucciones de manejo (2659 KB)
Referencias

HA/Hemagglutinin Protein, H1N1 (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HA/Hemagglutinin Protein, H1N1 (sf9, His)
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