HA/Hemagglutinin Protein, H1N1 (sf9, His)

HA/hemagglutinin proteins bind to sialic acid receptors and initiate viral attachment and internalization through clathrin-dependent or -independent pathways. This class I viral fusion protein determines viral host range and virulence. HA/Hemagglutinin Protein, H1N1 (sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HA/Hemagglutinin Protein, H1N1 (sf9, His) is 529 a.a., with molecular weight of ~66 kDa.

Binds to sialic acid-containing receptors on the cell surface, facilitating the attachment of the virus particle. This attachment leads to the internalization of the virion through either clathrin-dependent endocytosis or a clathrin- and caveolin-independent pathway. HA/Hemagglutinin Protein plays a crucial role in determining the host range restriction and virulence of the virus. It acts as a Class I viral fusion protein, enabling the penetration of the virus into the cell cytoplasm by mediating the fusion of the endocytosed virus particle's membrane with the endosomal membrane. The acidic pH in endosomes triggers a conformational change in HA2, causing the release of the fusion hydrophobic peptide. Multiple trimers are necessary to form a functional fusion pore.

Virus

Sf9 insect cells

C-His

C3W5S1 (M1-Q529)

/

MKAILVVLLYTFATANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADTYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPKGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNIPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREEIDGVKLESTRIYQ

Approximately 66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, pH 7.4, 300 mM NaCl, 5% mannitol, 5% trehalose.

<1 EU/μg; determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.