2. Membrane Transporter/Ion Channel
  3. ASCT
  4. V-9302

V-9302 is a competitive antagonist of transmembrane glutamine flux. V-9302 selectively and potently targets the amino acid transporter ASCT2 (SLC1A5) not ASCT1. V-9302 inhibits ASCT2-mediated glutamine uptake (IC50=9.6 μM) in HEK-293 cells.

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CAS 番号 : 1855871-76-9

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10 mM * 1 mL in DMSO
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Solid
5 mg $70 在庫あり
10 mg $100 在庫あり
25 mg $180 在庫あり
50 mg $280 在庫あり
100 mg $450 在庫あり
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カスタマーレビュー

Based on 34 publication(s) in Google Scholar

Other Forms of V-9302:

V-9302 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Imaging/Staining
In Vivo Efficacy Study
Others
IHC
Bio/Physico-chemical Assay

    V-9302 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 10:e07057.  [Abstract]

    V-9302 (50 μM, 35 min) suppressed the bioluminescent intensity of HCT116‐luc cells.

    V-9302 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 16:e2411479.  [Abstract]

    V9302 (s.c., 30 mg kg⁻¹, 20 days) in combination with LL202 (s.c., 30 mg kg⁻¹, 20 days) inhibited tumor growth in the H1975OR xenograft model.

    V-9302 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 10:e07057.  [Abstract]

    V-9302 (25 or 50 μM, 35 min) lowered the total photon flux induced by BL568 or AA201 in HCT116‐luc cells.

    V-9302 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 16:e2411479.  [Abstract]

    V9302 (s.c., 30 mg kg⁻¹, 20 days) in combination with LL202 (s.c., 30 mg kg⁻¹, 20 days) increased γH2AX expression level and the number of TUNEL‑positive cells in the H1975OR xenograft model.

    V-9302 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug 10:e07057.  [Abstract]

    V-9302 (25 μM, 70 min) reduced intracellular AA201 levels in all three cell lines.

    ASCT アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    ASCT2

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    V-9302 is a competitive antagonist of transmembrane glutamine flux. V-9302 selectively and potently targets the amino acid transporter ASCT2 (SLC1A5) not ASCT1. V-9302 inhibits ASCT2-mediated glutamine uptake (IC50=9.6 μM) in HEK-293 cells[1].

    IC50 & Target

    ASCT2

     

    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    20.46 μM
    Compound: V9302
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
    		[PMID: 38217503]
    C6 IC50
    9 μM
    Compound: 17
    Inhibition of ASCT2-mediated glutamine transport in rat C6 cells using [3H]-glutamine after 15 mins by scintillation counting
    Inhibition of ASCT2-mediated glutamine transport in rat C6 cells using [3H]-glutamine after 15 mins by scintillation counting
    		[PMID: 26750251]
    HEK293 IC50
    9.6 μM
    Compound: 17
    Inhibition of ASCT2-mediated glutamine transport in human HEK293 cells using [3H]-glutamine after 15 mins by scintillation counting
    Inhibition of ASCT2-mediated glutamine transport in human HEK293 cells using [3H]-glutamine after 15 mins by scintillation counting
    		[PMID: 26750251]
    体外実験

    V-9302 inhibits ASCT2-mediated glutamine uptake in human cells in a concentration-dependent fashion and exhibits a 100-fold improvement in potency over gamma-L-glutamyl-p-nitroanilide[1].
    Pharmacological blockade of ASCT2 with V-9302 results in attenuated cancer cell growth and proliferation, increases cell death, and increases oxidative stress[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    V-9302 関連抗体
    体内実験

    V-9302 (75 mg/kg; i.p.; daily fo 21 days) prevents tumor growth in both HCT-116 and HT29 xenograft models[1].
    The combination of CB-839 and V-9302 (30 mg/kg; i.p.; SNU398 and MHCC97H cells were grown as tumor xenografts in BALB/c nude mice; for 20 or 15 d, respectively) elicits a strong growth inhibition in both SNU398 and MHCC97H xenograft models, while single-drug treatment showed modest anti-tumor effects[2].
    V-9302 (50 mg/kg ; i.p.; daily for 5 days) displays markedly reduced tumor growth[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 6-week old, female athymic nude mice (bearing HCT-116 (KRAS G13D) or HT29 (BRAF V600E) cell-line)[1]
    Dosage: 75 mg/kg
    Administration: Intraperitoneally; daily fo 21 days
    Result: Prevented tumor growth.
    分子量

    538.68

    分子式

    C34H38N2O4
    CAS 番号
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C(O)[C@@H](N)CCN(CC1=CC=CC=C1OCC2=CC=CC(C)=C2)CC3=CC=CC=C3OCC4=CC=CC(C)=C4
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : 25 mg/mL (46.41 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8564 mL 9.2819 mL 18.5639 mL
    5 mM 0.3713 mL 1.8564 mL 3.7128 mL
    10 mM 0.1856 mL 0.9282 mL 1.8564 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

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    濃度 （終了）

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    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1 mg/mL (1.86 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 1 mg/mL (1.86 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.46%

    COA (257 KB) HNMR (279 KB) RP-HPLC (256 KB) MS (205 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8564 mL 9.2819 mL 18.5639 mL 46.4097 mL
    5 mM 0.3713 mL 1.8564 mL 3.7128 mL 9.2819 mL
    10 mM 0.1856 mL 0.9282 mL 1.8564 mL 4.6410 mL
    15 mM 0.1238 mL 0.6188 mL 1.2376 mL 3.0940 mL
    20 mM 0.0928 mL 0.4641 mL 0.9282 mL 2.3205 mL
    25 mM 0.0743 mL 0.3713 mL 0.7426 mL 1.8564 mL
    30 mM 0.0619 mL 0.3094 mL 0.6188 mL 1.5470 mL
    40 mM 0.0464 mL 0.2320 mL 0.4641 mL 1.1602 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    V-9302 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    V-93021855871-76-9V9302V 9302ASCTAlanine Serine Cysteine TransportersglutamineuptakefluxtransporterproliferationoxidativestressathymicnudemiceHEK-293Inhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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