2. Metabolic Enzyme/Protease Apoptosis Autophagy
  3. Hexokinase Apoptosis Autophagy
  4. 3-Bromopyruvic acid

3-Bromopyruvic acid  (Synonyms: 3-BP; Bromopyruvic acid; 3-ブロモピルビン酸)

製品番号: HY-19992 純度: 98.0%
COA 取扱説明書 Technical Support

3-Bromopyruvate (Bromopyruvic acid) is an analogue of pyruvate and a potent hexokinase (HK)-II inhibitor with high tumor selectivity. 3-Bromopyruvate inhibits cell growth and induces apoptosis through interfering with glycolysis. 3-Bromopyruvate induces autophagy by stimulating ROS formation in breast cancer cells. Antimicrobial activities.

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CAS 番号 : 1113-59-3

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
500 mg $50 在庫あり
1 g $60 在庫あり
5 g $72 在庫あり
10 g $96 在庫あり
25 g $192 在庫あり
50 g   お問い合わせ  

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カスタマーレビュー

Based on 32 publication(s) in Google Scholar

3-Bromopyruvic acid 関連抗体

Top Publications Citing Use of Products

顧客検証

IP
WB
IHC

    3-Bromopyruvic acid purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Mar 1.

    Representative TEM images showed that IR780/3BP@PLGA and CSIR780/3BP@PLGA (Groups 4 and 6, 1 mg/mL PLGA concentration) nanoparticles presented a spherical structure.

    3-Bromopyruvic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Mar 17;16(1):182.  [Abstract]

    Co-IP was performed to clarify the relationship between HK2 and HMGCS1 binding with p62 when added in 10 μM 3-BrPA (3-Bromopyruvic acid) in KLE cells.

    3-Bromopyruvic acid purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Aug 28;12(1):49.  [Abstract]

    Immunoblots of the pre-proenzyme and active CTSK levels in osteoclasts treated with 2DG (1 μM), FCCP (1 μM), Rotenone (100 nmol/L) or 3-BrPA (3-Bromopyruvic acid, 1 μM) for 10 h. The MMP-9 and CTSB expression levels were also detected. DMSO-treated osteoclasts were used as controls.

    3-Bromopyruvic acid purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Aug 28;12(1):49.  [Abstract]

    Immunofluorescence staining to show the CTSK expression level in osteoclasts that were treated with Oligomycin A (10 nM), Rotenone (100 nM), FCCP (1 μM), 2DG (1 μM) or 3BrPA (3-Bromopyruvic acid, 1 μM) for 6 h.

    3-Bromopyruvic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Oct 30:137070.  [Abstract]

    3-BPA (3-Bromopyruvic acid) at concentrations from 1.1 to 10 μM and GSK at concentrations from 3.3 to 30 μM inhibited ASFV replication in a dose-dependent manner, indicating that glycolysis inhibition leads to suppression of ASFV replication in PAMs.

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    製品説明

    3-Bromopyruvate (Bromopyruvic acid) is an analogue of pyruvate and a potent hexokinase (HK)-II inhibitor with high tumor selectivity. 3-Bromopyruvate inhibits cell growth and induces apoptosis through interfering with glycolysis. 3-Bromopyruvate induces autophagy by stimulating ROS formation in breast cancer cells. Antimicrobial activities[1][2][3].

    Cellular Effect
    Cell Line Type Value Description References
    A549 GI50
    25 3
    Compound: 3-BP
    Growth inhibition of human A549 cells expressing MCT1 incubated for 72 hrs by MTT assay
    Growth inhibition of human A549 cells expressing MCT1 incubated for 72 hrs by MTT assay
    		[PMID: 36584238]
    A549 GI50
    25 3
    Compound: 3-BP
    Growth inhibition of human A549 cells expressing MCT1 incubated for 72 hrs by MTT assay
    Growth inhibition of human A549 cells expressing MCT1 incubated for 72 hrs by MTT assay
    		[PMID: 36584238]
    体外実験

    3-Bromopyruvate enhances TRAIL-induced apoptosis in breast cancer cells[2].
    3-Bromopyruvate (Bromopyruvic acid), a hexokinase II inhibitor, can induce apoptosis in hepatocellular carcinoma cells by inducing endoplasmic reticulum (ER) stress[2].
    3-Bromopyruvate inhibits ATP generation and upregulates the expression of DR5. 3-Bromopyruvate upregulates CHOP, GRP78 and the phosphorylation of AMPK and augments TRAIL-induced Bax and caspase-3 levels[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    3-Bromopyruvic acid 関連抗体

    Cell Viability Assay[2]

    Cell Line: MCF-7 and MDA-MB-231 cells
    Concentration: 40, 80, 160 or 320 µM
    Incubation Time: 24 hours
    Result: 3-Bromopyruvate (80 and 160 µmol/l) and TRAIL (400 ng/ml) significantly inhibited cell viability.
    体内実験

    3-Bromopyruvate (8 mg/kg; i.p.; every 4 days for 28 days) shows a synergistic antitumor effect in MCF-7 cell xenografts in nude mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female nude mice (BALB/c; 4-5-weeks old and 18-20 g)[2]
    Dosage: 8 mg/kg
    Administration: I.p.; every 4 days for 28 days
    Result: Showed antitumor efficacy in tumor xenografts.
    分子量

    166.96

    分子式

    C3H3BrO3
    CAS 番号
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(O)C(CBr)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : 175 mg/mL (1048.16 mM; Need ultrasonic)

    DMSO : 100 mg/mL (598.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.9895 mL 29.9473 mL 59.8946 mL
    5 mM 1.1979 mL 5.9895 mL 11.9789 mL
    10 mM 0.5989 mL 2.9947 mL 5.9895 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体積 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (14.97 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (14.97 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (598.95 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 98.0%

    COA (245 KB) HNMR (212 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 5.9895 mL 29.9473 mL 59.8946 mL 149.7365 mL
    5 mM 1.1979 mL 5.9895 mL 11.9789 mL 29.9473 mL
    10 mM 0.5989 mL 2.9947 mL 5.9895 mL 14.9736 mL
    15 mM 0.3993 mL 1.9965 mL 3.9930 mL 9.9824 mL
    20 mM 0.2995 mL 1.4974 mL 2.9947 mL 7.4868 mL
    25 mM 0.2396 mL 1.1979 mL 2.3958 mL 5.9895 mL
    30 mM 0.1996 mL 0.9982 mL 1.9965 mL 4.9912 mL
    40 mM 0.1497 mL 0.7487 mL 1.4974 mL 3.7434 mL
    50 mM 0.1198 mL 0.5989 mL 1.1979 mL 2.9947 mL
    60 mM 0.0998 mL 0.4991 mL 0.9982 mL 2.4956 mL
    80 mM 0.0749 mL 0.3743 mL 0.7487 mL 1.8717 mL
    100 mM 0.0599 mL 0.2995 mL 0.5989 mL 1.4974 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    3-Bromopyruvic acid Related Classifications

    • Molarity Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    3-Bromopyruvic acid1113-59-3Bromopyruvic acid 3-BrPA Hexokinase II Inhibitor II, 3-BPHexokinaseApoptosisAutophagyanaloguepyruvateglycolysisERstressMCF-7MDA-MB-231cellsInhibitorinhibitorinhibit

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    製品名:
    3-Bromopyruvic acid
    製品番号:
    HY-19992
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