2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. 5,6-Dihydro-5-methyluracil

5,6-Dihydro-5-methyluracil  (Synonyms: Dihydrothymine)

Cat. No.: HY-N6787 Pureté: 99.88%
COA
SDS
Instruction de manipulation Technical Support

5,6-Dihydro-5-methyluracil (Dihydrothymine) is an intermediate breakdown product of Thymine (HY-W010450). 5,6-Dihydro-5-methyluracil can be used as a marker of DNA damage. 5,6-Dihydro-5-methyluracil can be used for the research of COVID-19.

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5,6-Dihydro-5-methyluracil

5,6-Dihydro-5-methyluracil Chemical Structure

CAS No. : 696-04-8

Size Prix Stock Quantité
Échantillon gratuit (0.1 - 0.2 mg)   Appliquer maintenant  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
50 mg En stock
100 mg En stock
250 mg En stock
500 mg   Obtenir un devis  
1 g   Obtenir un devis  

* Veuillez sélectionner la quantité avant d'ajouter des articles.

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Based on 3 publication(s) in Google Scholar

Other Forms of 5,6-Dihydro-5-methyluracil:

5,6-Dihydro-5-methyluracil Related Antibodies

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Human Endogenous Metabolite Microbial Metabolite

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Description

5,6-Dihydro-5-methyluracil (Dihydrothymine) is an intermediate breakdown product of Thymine (HY-W010450). 5,6-Dihydro-5-methyluracil can be used as a marker of DNA damage. 5,6-Dihydro-5-methyluracil can be used for the research of COVID-19[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

5,6-Dihydro-5-methyluracil X-irradiation of d(TpA) in anaerobic aqueous solution produces 5,6-dihydrothymine-modified d(TpA) as the most abundant lesion[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5,6-Dihydro-5-methyluracil Related Antibodies
Masse moléculaire

128.13

Formule

C5H8N2O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1NC(C(C)CN1)=O
Structure Classification
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvant et solubilité
In Vitro: 

DMSO : 62.5 mg/mL (487.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.8046 mL 39.0229 mL 78.0457 mL
5 mM 1.5609 mL 7.8046 mL 15.6091 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (16.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (16.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: 99.88%

SDS (393 KB)

COA (251 KB) HNMR (254 KB) RP-HPLC (243 KB)

Instruction de manipulation (2659 KB)
Références

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 7.8046 mL 39.0229 mL 78.0457 mL 195.1143 mL
5 mM 1.5609 mL 7.8046 mL 15.6091 mL 39.0229 mL
10 mM 0.7805 mL 3.9023 mL 7.8046 mL 19.5114 mL
15 mM 0.5203 mL 2.6015 mL 5.2030 mL 13.0076 mL
20 mM 0.3902 mL 1.9511 mL 3.9023 mL 9.7557 mL
25 mM 0.3122 mL 1.5609 mL 3.1218 mL 7.8046 mL
30 mM 0.2602 mL 1.3008 mL 2.6015 mL 6.5038 mL
40 mM 0.1951 mL 0.9756 mL 1.9511 mL 4.8779 mL
50 mM 0.1561 mL 0.7805 mL 1.5609 mL 3.9023 mL
60 mM 0.1301 mL 0.6504 mL 1.3008 mL 3.2519 mL
80 mM 0.0976 mL 0.4878 mL 0.9756 mL 2.4389 mL
100 mM 0.0780 mL 0.3902 mL 0.7805 mL 1.9511 mL
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5,6-Dihydro-5-methyluracil Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5,6-Dihydro-5-methyluracil696-04-8DihydrothymineEndogenous Metaboliteaqueous solutiond(TpA)thymineDNA lesionX-irradiated mammalian cellsanaerobic conditionsstereoisomersDNA damageCOVID-19Inhibitorinhibitorinhibit

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