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  4. Acridine Orange hydrochloride

Acridine Orange (hydrochloride)  (Synonyms: アクリジンオレンジ 塩酸塩)

製品番号: HY-101879 純度: 99.65%
COA 取扱説明書 Technical Support

Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).

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CAS 番号 : 65-61-2

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 在庫あり
Solution
10 mM * 1 mL in DMSO USD 66 在庫あり
Solid
100 mg $60 在庫あり
200 mg $95 在庫あり
500 mg $180 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 38 publication(s) in Google Scholar

Other Forms of Acridine Orange hydrochloride:

Acridine Orange (hydrochloride) 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Imaging/Staining
IF

    Acridine Orange hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 Mar;22(3):282-299.  [Abstract]

    Human PBMCs (H-PBMCs) and Jurkat cells were treated with OA (5, 50, or 500 μM) or 200 ng/mL PMA for 24 hours, after which the cells were stained with acridine orange (AO).

    Acridine Orange hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jun 30;14(1):3877.  [Abstract]

    Acridine Orange (AO, 5 μM) was added immediately after the ultrasound treatment and was incubated for 30 min at 37°C before confocal microscopy.

    Acridine Orange hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Oct;11(10):3178-3192.

    The Jurkat cells were treated with LW-218 (8 μmol/L) for 0, 1, 2, and 4 h. Before collecting, the cells were stained with 5 μmol/L Acridine Orange hydrochloride solution for 15 min.

    Acridine Orange hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Jan 13;12(1):80.  [Abstract]

    The Jurkat cells treated with 25 μM FV-429 for 12 h were stained with Acridine Orange hydrochloride (5 μM) before collection, and the AO fluorescence was determined by a laser confocal microscope.

    Acridine Orange hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2022 Jan;55(1):e13172.  [Abstract]

    CLSM images of acridine orange (AO)-stained osteoclasts cultured on PDMS substrates were taken; the cells were stained with 1 μM AO at 37°C for 20 min.

    Acridine Orange hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Jul 30;11(7):607.  [Abstract]

    Depending on the acidity, Acridine Orange hydrochloride (AO) causes autophagic lysosomes to appear as orange/red fluorescent vesicles, while it caused nuclei to appear green. AO staining demonstrates that LA dose-dependently increased the numbers of intracellular acidic compartments.

    Parasite アイソフォーム固有の製品をすべて表示:

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    Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

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    製品説明

    Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].

    Cellular Effect
    Cell Line Type Value Description References
    R3230AC IC50
    2.29 3
    Compound: AO.HCl
    Phototoxicity in rat R3230AC cells irradiated with halogen lamp pretreated 24 hrs before irradiation by MTT assay
    Phototoxicity in rat R3230AC cells irradiated with halogen lamp pretreated 24 hrs before irradiation by MTT assay
    		[PMID: 19692249]
    R3230AC IC50
    2.29 3
    Compound: AO.HCl
    Phototoxicity in rat R3230AC cells irradiated with halogen lamp pretreated 24 hrs before irradiation by MTT assay
    Phototoxicity in rat R3230AC cells irradiated with halogen lamp pretreated 24 hrs before irradiation by MTT assay
    		[PMID: 19692249]
    R3230AC IC50
    2.29 3
    Compound: AO.HCl
    Phototoxicity in rat R3230AC cells irradiated with halogen lamp pretreated 24 hrs before irradiation by MTT assay
    Phototoxicity in rat R3230AC cells irradiated with halogen lamp pretreated 24 hrs before irradiation by MTT assay
    		[PMID: 19692249]
    体外実験

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Working Solution: Dilute Acridine Orange hydrochloride stock in serum-free medium or PBS to a final concentration of 1-10 μM or 2-5 μg/mL.
    2. Incubation: Incubate cells for 15-30 min at 37 ℃.
    3. Washing: Wash cells twice with pre-warmed PBS.
    4. Visualization: Observe immediately using a fluorescent microscope (Excitation ≈ 488 nm, Green emission for DNA, Red/Orange for RNA/acidic vesicles).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acridine Orange (hydrochloride) 関連抗体
    体内実験

    Acridine Orange (0.1 mg/kg, i.v., dogs) hydrochloride shows no clinical signs of toxicity and no abnormalities were seen in the blood within 30 days[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    301.82

    分子式

    C17H20ClN3
    CAS 番号
    Appearance

    Solid

    Color

    Brown to red

    SMILES

    CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1.[H]Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
    溶剤 & 溶解度
    体外: 

    H2O : 50 mg/mL (165.66 mM; ultrasonic and heat to 60°C)

    DMSO : 25 mg/mL (82.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3132 mL 16.5662 mL 33.1323 mL
    5 mM 0.6626 mL 3.3132 mL 6.6265 mL
    10 mM 0.3313 mL 1.6566 mL 3.3132 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.86%

    COA (246 KB) HNMR (180 KB) LCMS (340 KB)

    取扱説明書 (2659 KB)
    Dyeing Example
    参考文献
    細胞実験

    Two-step, pH 3.0: Aliquots (0.2 mL, containing approximately 2-5x105 cells) are withdrawn from cultures and are added to 0.5 mL of a solution containing: 0.1% (v/v) Triton X-100, 0.2 M sucrose, 10-4 M EDTA and 2x10-2 M citrate-phosphate buffer, at pH 3.0. Triton X-100 is included in the various procedures at the indicated pH to increase cell permeability yet maintain cellular integrity. The chelating agent EDTA is used to facilitate RNA denaturation. The cells are stained one minute later by addition of 1 mL of a solution containing 0.002% (20 μg/mL) AO, 0.1 M NaCl and 10-2 M citrate-phosphate buffer, pH 3.8. Cations are included in the staining mixture to ensure staining specificity. The final AO concentration is approximately 4x10-5 M[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.3132 mL 16.5662 mL 33.1323 mL 82.8308 mL
    5 mM 0.6626 mL 3.3132 mL 6.6265 mL 16.5662 mL
    10 mM 0.3313 mL 1.6566 mL 3.3132 mL 8.2831 mL
    15 mM 0.2209 mL 1.1044 mL 2.2088 mL 5.5221 mL
    20 mM 0.1657 mL 0.8283 mL 1.6566 mL 4.1415 mL
    25 mM 0.1325 mL 0.6626 mL 1.3253 mL 3.3132 mL
    30 mM 0.1104 mL 0.5522 mL 1.1044 mL 2.7610 mL
    40 mM 0.0828 mL 0.4142 mL 0.8283 mL 2.0708 mL
    50 mM 0.0663 mL 0.3313 mL 0.6626 mL 1.6566 mL
    60 mM 0.0552 mL 0.2761 mL 0.5522 mL 1.3805 mL
    80 mM 0.0414 mL 0.2071 mL 0.4142 mL 1.0354 mL
    H2O 100 mM 0.0331 mL 0.1657 mL 0.3313 mL 0.8283 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Acridine Orange hydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acridine Orange65-61-2DNA StainParasiteFluorescent DyeNucleic aciddsDNARNAssDNAInhibitorinhibitorinhibit

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    製品番号:
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