Acridine Orange hydrochloride (solution) 

Acridine Orange hydrochloride (solution) is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm) ^[1][2][3].
Solvent and Concentration: Sterile water: 10 mg/mL
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[4].
1. Stain cells with Acridine Orange hydrochloride (1 μM; 20 min; 37°C).
2. Wash cells with PBS.
3. Cells are observed by a confocal laser scanning microscopy (FV3000, Olympus).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Acridine Orange (0.1 mg/kg, i.v., dogs) hydrochloride shows no clinical signs of toxicity and no abnormalities were seen in the blood within 30 days^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

301.82

C₁₇H₂₀ClN₃

65-61-2

Liquid

Orange to red

CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1.[H]Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925.  [Content Brief]

  [2]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172.  [Content Brief]

  [3]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.  [Content Brief]

  [4]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.  [Content Brief]