Arctiin (Synonyms: Arctii; NSC 315527; Arctigenin-4-glucoside)

Name: Arctiin
Brand: MedChemExpress
SKU: HY-N0034
Rating: 5.0 (5 reviews)

Cat. No.: HY-N0034 Pureza: 99.52%: Ficha de datos
COA

SDS
Instrucciones de manejo
FAQs
Technical Support

Arctiin is an orally active inhibitor of NF-κB. Arctiin suppresses cyclin D1 protein expression in human tumor cells. Arctiin also reduces malondialdehyde and pro-in ammatory cytokines levels. Arctiin can used in study glomerulonephritis.

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 20362-31-6

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

This product is a controlled substance and not for sale in your territory.

Revisión del cliente

Based on 5 publication(s) in Google Scholar

Other Forms of Arctiin:

Arctiin (Standard) Obtener un presupuesto

5 Publications Citing Use of MCE Arctiin

RT-PCR

Apoptosis Analysis

ELISA

Flow Cytometry

: Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927. [Abstract]; Protein immunoblot analysis was performed to examine the expression of AdipoR1 in the retina of EAU mice at day 20 treated with Arctiin (1, 10 μM).

: Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927. [Abstract]; The mRNA expression of T cell-related inflammatory factors in the retina was determined by RT-PCR treated with Arctiin (10 μM).

: Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927. [Abstract]; CD4⁺ T cells were treated with DMSO or Arctiin (0.1, 1 and 10 μM) for 48 h, then cytotoxicity of Arctiin was detected by apoptosis assay.

: Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927. [Abstract]; The protein expression of IFN-γ, IL-17 A and IL-10 in culture supernatant was determined by ELISA treated with Arctiin (10 μM).

: Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927. [Abstract]; CFSE labled CD4⁺ T cells were treated with DMSO or Arctiin (10 μM) for 96 h, then cell proliferation was detected by flow cytometry.

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas NF-κB:

Ver todas las isoformas

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Cellular Effect

Cell Line	Type	Value	Description	References
MRC5	CC₅₀	240 μM Compound: 8	Cytotoxicity against MRC5 cells Cytotoxicity against MRC5 cells	[PMID: 17239594]
MRC5	IC₅₀	16 μM Compound: 8	Antiviral activity against HCMV in MRC5 cells by plaque reduction assay Antiviral activity against HCMV in MRC5 cells by plaque reduction assay	[PMID: 17239594]
RAW264.7	IC₅₀	>0.3 mM Compound: 22	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs	[PMID: 18986199]
RAW264.7	IC₅₀	>0.3 μM/mL Compound: 22	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs	[PMID: 18986199]

In Vitro

Arctiin (100 μM) down-regulates cyclin D1 protein expression in human tumor cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[6]

Cell Line:	HaCaT, MG63, A549, HCT116, HeLa, MCF-7, and 293T cell line
Concentration:	10, 25,100 and 250 µM
Incubation Time:	0, 6, 12, 24 and 48 h
Result:	Inhibited the HaCaT cells growth with 100 µM at 48 h. Significantly repressed the expression of the cyclin D1 protein with 100 µM in all tumor cells. Dephosphorylated RB protein in HaCaT cells at 48 h.

In Vivo

Arctiin (120 mg/kg for p.o.;Once daily for 3 weeks) significantly improves cBSA-induced glomerulonephritis in glomerulonephritis rat model^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Glomerulonephritis rat model^[4]
Dosage:	30, 60, and 120 mg/kg
Administration:	Oral gavage (p.o.);Once daily for 3 weeksv
Result:	Significantly decreased the levels of urine protein. Markedly ameliorated the renal function. Dramatically reduced IL-6 and TNF-a concentration in rat serum. Dramatically decreased the NF-kB DNA binding activity. Significantly reduced the MDA level and increased the SOD activity.

Peso molecular

534.55

Fòrmula

C₂₇H₃₄O₁₁

No. CAS

20362-31-6

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](OC2=C(OC)C=C(C[C@H]3C(OC[C@@H]3CC4=CC(OC)=C(OC)C=C4)=O)C=C2)[C@@H]1O

Structure Classification

Initial Source

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvente y solubilidad

In Vitro:

DMSO : 200 mg/mL (374.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (18.71 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8707 mL	9.3537 mL	18.7073 mL
5 mM	0.3741 mL	1.8707 mL	3.7415 mL
10 mM	0.1871 mL	0.9354 mL	1.8707 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 10 mg/mL (18.71 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.52%

Select Batch:

Ficha de datos (285 KB) SDS (393 KB)

COA (259 KB) LCMS (88 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Huang DM, et al. Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells. Prostate. 2004 May 15;59(3):260-7. [Content Brief]

[2]. Knipping K, et al. In vitro and in vivo anti-allergic effects of Arctium lappa L. Exp Biol Med (Maywood). 2008 Nov;233(11):1469-77. [Content Brief]

[3]. Zeng Y, et al. Lack of significant modifying effect of arctiin on prostate carcinogenesis in probasin/SV40 T antigen transgenic rats. Cancer Lett. 2005 May 26;222(2):145-51. [Content Brief]

[4]. Wu JG, et al. Ameliorative effects of arctiin from Arctium lappa on experimental glomerulonephritis in rats. Phytomedicine. 2009 Nov;16(11):1033-41. [Content Brief]

[5]. Ma ST, et al. Effect of arctiin on glomerular filtration barrier damage in STZ-induced diabetic nephropathy rats. Phytother Res. 2013 Oct;27(10):1474-80. [Content Brief]

[6]. Matsuzaki Y, et al. Arctiin induces cell growth inhibition through the down-regulation of cyclin D1 expression. Oncol Rep. 2008 Mar;19(3):721-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.8707 mL	9.3537 mL	18.7073 mL	46.7683 mL
	5 mM	0.3741 mL	1.8707 mL	3.7415 mL	9.3537 mL
	10 mM	0.1871 mL	0.9354 mL	1.8707 mL	4.6768 mL
	15 mM	0.1247 mL	0.6236 mL	1.2472 mL	3.1179 mL
DMSO	20 mM	0.0935 mL	0.4677 mL	0.9354 mL	2.3384 mL
	25 mM	0.0748 mL	0.3741 mL	0.7483 mL	1.8707 mL
	30 mM	0.0624 mL	0.3118 mL	0.6236 mL	1.5589 mL
	40 mM	0.0468 mL	0.2338 mL	0.4677 mL	1.1692 mL
	50 mM	0.0374 mL	0.1871 mL	0.3741 mL	0.9354 mL
	60 mM	0.0312 mL	0.1559 mL	0.3118 mL	0.7795 mL
	80 mM	0.0234 mL	0.1169 mL	0.2338 mL	0.5846 mL
	100 mM	0.0187 mL	0.0935 mL	0.1871 mL	0.4677 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Arctiin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Productos vistos recientemente:

Nombre del producto

Requested Quantity *

Nombre del solicitante *

Saludo

Direcciòn del E-mail *

Número de teléfono *

Department

Nombre de la Organizaciòn *

City

Provincia

Country or Region *

Observaciones

Nombre del producto

Lot #

Nombre del solicitante *

Direcciòn del E-mail *

Número de teléfono

Department *

Nombre de la Organizaciòn *

Country or Region *

Observaciones

Name
E-mail *

	Sorry, but the email address you supplied was invalid.

Arctiin (Synonyms: Arctii; NSC 315527; Arctigenin-4-glucoside)

Revisión del cliente

Other Forms of Arctiin:

Arctiin Related Antibodies

5 Publications Citing Use of MCE Arctiin

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas NF-κB:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Arctiin Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Arctiin Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report