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Baicalin  (Synonyms: Baicalein 7-O-β-D-glucuronide)

Cat. No.: HY-N0197 Purity: 99.17%
COA Handling Instructions

Baicalin, as a flavonoid glycoside, is an allosteric carnitine palmityl transferase 1 (CPT1) activator. Baicalin reduces the expression of NF-κB.

For research use only. We do not sell to patients.

Baicalin Chemical Structure

Baicalin Chemical Structure

CAS No. : 21967-41-9

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10 mM * 1 mL in DMSO
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Customer Review

Based on 18 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Baicalin purchased from MedChemExpress. Usage Cited in: Oral Dis. 2019 Nov;25(8):1945-1953.  [Abstract]

    PF and BAI combination suppresses activation of the NF-κB signaling pathway in LPS-treated HOKs. Western blotting assay is performed to investigate expression of NF-κB inflammatory signaling proteins.

    Baicalin purchased from MedChemExpress. Usage Cited in: Oral Dis. 2019 Nov;25(8):1945-1953.  [Abstract]

    The nuclear translocation of NF-κB p65 was visualized using a laser confocal scanning microscope imaging system. Nuclei detected with DAPI (blue). Cells expressing NF-κB p65 in the cytoplasm (green). NF-κB p65 expresses mostly in the nucleus, and a small amount expressed in the cytoplasm (green). Localization of NF-κB p65 in the cytoplasm (green).

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    • Biological Activity

    • Protocol

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    • References

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    Description

    Baicalin, as a flavonoid glycoside, is an allosteric carnitine palmityl transferase 1 (CPT1) activator. Baicalin reduces the expression of NF-κB[1][2][3].

    IC50 & Target[1]

    NF-κB

     

    Autophagy

     

    In Vitro

    Baicalin protects against ischemia-reperfusion injury (IRI) by altering the production of various mediators, including reactive oxygen species (ROS), Toll-like receptor (TLR)2 and TLR4, NF-κB, Bax, and Bcl-2. Baicalin treatment inhibits the increased expression of the proinflammatory cytokines TLR2/4, MyD88, p-NF-κB, and p- IκB, as well as increase the expression of IκB protein, an NF-κB inhibitor[1].
    Cell viability is determined by MTT assay. Compared with control cells, cell viability is significantly decreased in SH-SY5Y cells treated with thrombin. Pre-treatment with Baicalin (5, 10, 20 μM) increases cell viability in a dose-dependent manner compared with cells treated thrombin alone[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Baicalin pretreatment dose-dependently protects against a loss of renal function, with the two higher doses (10 and 100 mg/kg) significantly decreasing Scr and blood urea nitrogen (BUN) concentrations. Tissue injury, as assessed using a 0-3 point scoring system, is lower for the Baicalin treated groups than for the ischemia-reperfusion (IR)+saline group. Compared with the sham group, malondialdehyde (MDA) content is only slightly up-regulated and the SOD activity is only slightly down-regulated in rats treated with 10 and 100 mg/kg Baicalin, indicating that Baicalin abrogates the increase in oxidative stress following reperfusion[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    446.36

    Formula

    C21H18O11

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O[C@H]([C@H]([C@@H]([C@@H](C(O)=O)O1)O)O)[C@@H]1OC2=C(O)C(O)=C3C(C=C(C4=CC=CC=C4)OC3=C2)=O

    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (224.03 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2403 mL 11.2017 mL 22.4034 mL
    5 mM 0.4481 mL 2.2403 mL 4.4807 mL
    10 mM 0.2240 mL 1.1202 mL 2.2403 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 20 mg/mL (44.81 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.60 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (5.60 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.17%

    References
    Cell Assay
    [2]

    SH-SY5Y cell lines are cultured in RPMI-1640 medium supplemented with 15% fetal bovine serum at 37°C in an air atmosphere containing 95% air and 5% CO2 with a saturated humidity. Upon a confluence of 60~70%, the SH-SY5Y cells are divided into: (i) control group, incubated in RPMI-1640 medium; (ii) thrombin group, which is subject to thrombin induction (40 U/L) for 6 h based on our pre-experiment; and (iii) Baicalin groups, which are treated by Baicalin (5 μM, 10 μM, or 20 μM) for 2 h before induction of thrombin. Cell viability is measured using MTT assay. Briefly, 15 μL of the MTT solution (5 mg/mL) is added to each well and incubated for 4 h at 37°C. After removing the supernatant, 80 μL DMSO are added into each well. The absorbance is measured at 492 nm using a microplate reader. All experiments are performed in triplicate[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    Rats[1]
    Male Wistar rats weighing 200-250 g are used. Rats are randomly divided into five groups of six rats each: (i) sham group; (ii) IR+saline group; (iii) IR+Baicalin (1 mg/kg) group; (iv) IR+Baicalin (10 mg/kg) group; and (v) IR+Baicalin (100 mg/kg) group. Renal IRI is induced by clamping the left renal artery for 45 min plus a right nephrectomy. Rats are anesthetized through an intraperitoneal injection of pentobarbital sodium (40 mg/kg body weight). After a median abdominal incision, the left renal arteries are clamped for 45 min with serrefine. After clamp removal, adequate restoration of blood flow is checked before abdominal closure. The right kidney is then removed. Sham-operated animals underwent the same surgical procedure without clamping[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Baicalin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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