2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. CU-CPT-9a

CU-CPT-9a is a specific TLR8 antagonist, with an IC50 of 0.5 nM.

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CAS 番号 : 2165340-32-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 110 在庫あり
Solution
10 mM * 1 mL in DMSO USD 110 在庫あり
Solid
5 mg $100 在庫あり
10 mg $160 在庫あり
25 mg $340 在庫あり
50 mg $550 在庫あり
100 mg $850 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 5 publication(s) in Google Scholar

CU-CPT-9a 関連抗体

Top Publications Citing Use of Products

    CU-CPT-9a purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 May;14(5):e70083.  [Abstract]

    m6A levels in EVs regulate inflammatory responses via TLR8 in macrophages. Differentiated THP‐1 cells (dTHP‐1) were pretreated with or without CU‐CPT‐9a (HY-112667; 48 h) and CU‐CPT‐4a (HY-108473; 48 h). Whole‐cell lysates obtained from dTHP‐1 cells were subjected to Western blot analysis using an anti‐phospho p65 antibody, anti‐p65 antibody and anti‐β‐actin antibody. Representative images from three independent experiments are shown.

    CU-CPT-9a purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 May;14(5):e70083.  [Abstract]

    m6A levels in EVs regulate inflammatory responses via TLR8 in macrophages. Differentiated THP‐1 cells (dTHP‐1) were pretreated with or without CU‐CPT‐9a (HY-112667; 48 h) and CU‐CPT‐4a (HY-108473; 48 h). Conditioned medium from dTHP‐1 cells treated with CCD‐841‐CoN or HT29 small EVs was used for ELISA. dTHP‐1 cells were pre‐transfected with or without TLR8 siRNA.

    CU-CPT-9a purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Apr 11;23(1):181.  [Abstract]

    BMDMs were pretreated with a TLR2 inhibitor (C29; HY-100461; 10 µM), TLR4 inhibitor (TAK242; HY-11109; 10 µM), TLR7 inhibitor (IRS661, 1 µM), TLR8 inhibitor (CU-CPT9a; HY-112667; 10 µM), or TLR9 inhibitor (IRS869, 1 µM) for 1 h, and then treated with NETs (500 ng/mL) for 3 h. The levels of Il1b, Il6 were measured by qPCR (n = 6).

    CU-CPT-9a purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Apr 11;23(1):181.  [Abstract]

    BMDMs were pretreated with a TLR2 inhibitor (C29; HY-100461; 10 µM), TLR4 inhibitor (TAK242; HY-11109; 10 µM), TLR7 inhibitor (IRS661, 1 µM), TLR8 inhibitor (CU-CPT9a; HY-112667; 10 µM), or TLR9 inhibitor (IRS869, 1 µM) for 1 h, and then treated with NETs (500 ng/mL) for 3 h. The level of Tnfa mRNA was measured by qPCR (n = 6).

    CU-CPT-9a purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2019 Mar 5:846:12-22.  [Abstract]

    The effect of CU-CPT-9a on the protein levels of MyD88 and p38.

    Toll-like Receptor (TLR) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

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    • 純度とドキュメンテーション

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    製品説明

    CU-CPT-9a is a specific TLR8 antagonist, with an IC50 of 0.5 nM.

    IC50 & Target[1]

    TLR8

    0.5 nM (IC50)

    体外実験

    CU-CPT-9a is a specific TLR8 antagonist, with an IC50 of 0.5±0.1 nM. The elevation of the downstream protein levels induced by R848 can be reversed by CU-CPT-9a in a dose-dependent manner. By contrast, the expression of TRIF and IRF3 (cytoplasmic and nuclear) are only responsive to TLR4 and TLR3, independent of TLR837. The expression levels of TRIF and IRF3 do not show significant change in THP-1 cells upon treatment of R848, nor do they change with the treatment of CU-CPT-9a. CU-CPT8m and CU-CPT-9a both significantly suppress the TNF-α level in a dose-dependent manner, which is in agreement with previous reports of TLR8 involvement in these autoimmune diseases[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CU-CPT-9a 関連抗体
    分子量

    265.31

    分子式

    C17H15NO2
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    OC1=C(C)C=C(C=C1)C2=CC=NC3=CC(OC)=CC=C32
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 125 mg/mL (471.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7692 mL 18.8459 mL 37.6918 mL
    5 mM 0.7538 mL 3.7692 mL 7.5384 mL
    10 mM 0.3769 mL 1.8846 mL 3.7692 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.74%

    COA (252 KB) HNMR (264 KB) LCMS (94 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.7692 mL 18.8459 mL 37.6918 mL 94.2294 mL
    5 mM 0.7538 mL 3.7692 mL 7.5384 mL 18.8459 mL
    10 mM 0.3769 mL 1.8846 mL 3.7692 mL 9.4229 mL
    15 mM 0.2513 mL 1.2564 mL 2.5128 mL 6.2820 mL
    20 mM 0.1885 mL 0.9423 mL 1.8846 mL 4.7115 mL
    25 mM 0.1508 mL 0.7538 mL 1.5077 mL 3.7692 mL
    30 mM 0.1256 mL 0.6282 mL 1.2564 mL 3.1410 mL
    40 mM 0.0942 mL 0.4711 mL 0.9423 mL 2.3557 mL
    50 mM 0.0754 mL 0.3769 mL 0.7538 mL 1.8846 mL
    60 mM 0.0628 mL 0.3141 mL 0.6282 mL 1.5705 mL
    80 mM 0.0471 mL 0.2356 mL 0.4711 mL 1.1779 mL
    100 mM 0.0377 mL 0.1885 mL 0.3769 mL 0.9423 mL
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    CU-CPT-9a Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CU-CPT-9a2165340-32-7Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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    製品名:
    CU-CPT-9a
    製品番号:
    HY-112667
    数量:
    MCE 日本正規代理店:

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