CU-CPT-9a

Name: CU-CPT-9a
Brand: MedChemExpress
SKU: HY-112667
Rating: 5.0 (5 reviews)

Cat. No.: HY-112667 Purity: 99.74%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

CU-CPT-9a is a specific TLR8 antagonist, with an IC₅₀ of 0.5 nM.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 2165340-32-7

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 5 publication(s) in Google Scholar

5 Publications Citing Use of MCE CU-CPT-9a

RT-PCR

ELISA

: CU-CPT-9a purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 May;14(5):e70083. [Abstract]; m6A levels in EVs regulate inflammatory responses via TLR8 in macrophages. Differentiated THP‐1 cells (dTHP‐1) were pretreated with or without CU‐CPT‐9a (HY-112667; 48 h) and CU‐CPT‐4a (HY-108473; 48 h). Whole‐cell lysates obtained from dTHP‐1 cells were subjected to Western blot analysis using an anti‐phospho p65 antibody, anti‐p65 antibody and anti‐β‐actin antibody. Representative images from three independent experiments are shown.

: CU-CPT-9a purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 May;14(5):e70083. [Abstract]; m6A levels in EVs regulate inflammatory responses via TLR8 in macrophages. Differentiated THP‐1 cells (dTHP‐1) were pretreated with or without CU‐CPT‐9a (HY-112667; 48 h) and CU‐CPT‐4a (HY-108473; 48 h). Conditioned medium from dTHP‐1 cells treated with CCD‐841‐CoN or HT29 small EVs was used for ELISA. dTHP‐1 cells were pre‐transfected with or without TLR8 siRNA.

: CU-CPT-9a purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Apr 11;23(1):181. [Abstract]; BMDMs were pretreated with a TLR2 inhibitor (C29; HY-100461; 10 µM), TLR4 inhibitor (TAK242; HY-11109; 10 µM), TLR7 inhibitor (IRS661, 1 µM), TLR8 inhibitor (CU-CPT9a; HY-112667; 10 µM), or TLR9 inhibitor (IRS869, 1 µM) for 1 h, and then treated with NETs (500 ng/mL) for 3 h. The levels of Il1b, Il6 were measured by qPCR (n = 6).

: CU-CPT-9a purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Apr 11;23(1):181. [Abstract]; BMDMs were pretreated with a TLR2 inhibitor (C29; HY-100461; 10 µM), TLR4 inhibitor (TAK242; HY-11109; 10 µM), TLR7 inhibitor (IRS661, 1 µM), TLR8 inhibitor (CU-CPT9a; HY-112667; 10 µM), or TLR9 inhibitor (IRS869, 1 µM) for 1 h, and then treated with NETs (500 ng/mL) for 3 h. The level of Tnfa mRNA was measured by qPCR (n = 6).

: CU-CPT-9a purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2019 Mar 5:846:12-22. [Abstract]; The effect of CU-CPT-9a on the protein levels of MyD88 and p38.

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TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

Biological Activity
순도&문서
References
고객리뷰

제품 설명

CU-CPT-9a is a specific TLR8 antagonist, with an IC₅₀ of 0.5 nM.

IC₅₀ & Target^[1]

TLR8

0.5 nM (IC₅₀)

In Vitro

CU-CPT-9a is a specific TLR8 antagonist, with an IC₅₀ of 0.5±0.1 nM. The elevation of the downstream protein levels induced by R848 can be reversed by CU-CPT-9a in a dose-dependent manner. By contrast, the expression of TRIF and IRF3 (cytoplasmic and nuclear) are only responsive to TLR4 and TLR3, independent of TLR837. The expression levels of TRIF and IRF3 do not show significant change in THP-1 cells upon treatment of R848, nor do they change with the treatment of CU-CPT-9a. CU-CPT8m and CU-CPT-9a both significantly suppress the TNF-α level in a dose-dependent manner, which is in agreement with previous reports of TLR8 involvement in these autoimmune diseases^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

265.31

화학식

C₁₇H₁₅NO₂

CAS No.

2165340-32-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=C(C)C=C(C=C1)C2=CC=NC3=CC(OC)=CC=C32

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

용액&용해도

In Vitro:

DMSO : 125 mg/mL (471.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7692 mL	18.8459 mL	37.6918 mL
5 mM	0.7538 mL	3.7692 mL	7.5384 mL
10 mM	0.3769 mL	1.8846 mL	3.7692 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.74%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (252 KB) HNMR (264 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang S, et al. Small-molecule inhibition of TLR8 through stabilization of its resting state. Nat Chem Biol. 2018 Jan;14(1):58-64. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7692 mL	18.8459 mL	37.6918 mL	94.2294 mL
	5 mM	0.7538 mL	3.7692 mL	7.5384 mL	18.8459 mL
	10 mM	0.3769 mL	1.8846 mL	3.7692 mL	9.4229 mL
	15 mM	0.2513 mL	1.2564 mL	2.5128 mL	6.2820 mL
	20 mM	0.1885 mL	0.9423 mL	1.8846 mL	4.7115 mL
	25 mM	0.1508 mL	0.7538 mL	1.5077 mL	3.7692 mL
	30 mM	0.1256 mL	0.6282 mL	1.2564 mL	3.1410 mL
	40 mM	0.0942 mL	0.4711 mL	0.9423 mL	2.3557 mL
	50 mM	0.0754 mL	0.3769 mL	0.7538 mL	1.8846 mL
	60 mM	0.0628 mL	0.3141 mL	0.6282 mL	1.5705 mL
	80 mM	0.0471 mL	0.2356 mL	0.4711 mL	1.1779 mL
	100 mM	0.0377 mL	0.1885 mL	0.3769 mL	0.9423 mL

CU-CPT-9a Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CU-CPT-9a2165340-32-7Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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CU-CPT-9a

고객리뷰

CU-CPT-9a Related Antibodies

5 Publications Citing Use of MCE CU-CPT-9a

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Toll-like Receptor (TLR) Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

CU-CPT-9a Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

CU-CPT-9a Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report