2. 疾患モデリングル製品 Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. Immunology and Inflammatory Disease Models VD/VDR Endogenous Metabolite
  4. Hapten
  5. Calcifediol

Calcifediol  (Synonyms: カルシフェジオール; 25-hydroxy Vitamin D3)

製品番号: HY-32351 純度: 99.94%
COA 取扱説明書 Technical Support

Calcifediol (25-hydroxy Vitamin D3) is an effective VDR ligand and VD supplement. Calcifediol is a prohormone of the vitamin D endocrine system (VDES) and is hydroxylated in the liver to produce the active form, calcitriol. Calcifediol can rapidly increase serum VD levels.

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CAS 番号 : 19356-17-3

容量 価格（税別） 在庫状況 数量
1 mg $57 在庫あり
5 mg $110 在庫あり
10 mg $180 在庫あり
25 mg $360 在庫あり
50 mg $580 在庫あり
100 mg $890 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 11 publication(s) in Google Scholar

Other Forms of Calcifediol:

Calcifediol 関連抗体

Top Publications Citing Use of Products

    Calcifediol purchased from MedChemExpress. Usage Cited in: J Clin Endocrinol Metab. 2026 Jan 5:dgaf708.  [Abstract]

    Effects of cholecalciferol and calcifediol treatment on ME-eSC decidualization. ME-eSCs were pre-treated with vehicle (Veh) vs. cholecalciferol at Veh vs. Calcifediol at 0.1 μM 4 hrs prior to decidualization (stimulated with cAMP+MPA).

    Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985.

    Western blot detection of p-P65Ser311, P65, TNFα, MCP-1, KIM1, and NGAL expression in cells after administration of the VDR agonist Calcifediol and/or DKS26-treated mRETCs; n = 3. Calcifediol: 300 nM, DKS26: 100 μg/mL.

    Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985.

    Effects of Calcifediol or DKS26 on the nuclear translocation of p-P65Ser311 (red) observed by laser confocal microscopy.

    Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985.

    Expression of p-IKKβ, IKKβ, p-IKBα, and IKBα after treatment with the VDR agonist calcifediol or DKS26 in mRTECs; n = 3. Calcifediol: 300 nM, DKS26: 100 μg/mL.

    Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2017 Dec 19;18(12). pii: E2764.  [Abstract]

    Effect of vitamin D and analogues on Interleukin-1β level. Interleukin-1β (IL-1β) level was determined by enzyme-linked immunosorbent assay (ELISA) technique (n ≥ 3). IL-1β was analyzed in SH-SY5Y wt cells incubated with 100 nM Calcifediol or analogues compared to cells treated with the solvent control.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Calcifediol (25-hydroxy Vitamin D3) is an effective VDR ligand and VD supplement. Calcifediol is a prohormone of the vitamin D endocrine system (VDES) and is hydroxylated in the liver to produce the active form, calcitriol. Calcifediol can rapidly increase serum VD levels[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    体外実験

    Calcifediol in either liposomes or ethanolic solution has no effect on the release of the proinflammatory cytokine KC from Pseudomonas-infected murine epithelial cells. Treatment of infected, human bronchial 16-HBE cells with Calcifediol liposomes results in a significant reduction in bacterial survival[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Calcifediol 関連抗体
    分子量

    400.64

    分子式

    C27H44O2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C=C(CC1)/C(C[C@H]1O)=C\C=C2[C@@]3([H])[C@@](CCC\2)(C)[C@@H]([C@H](C)CCCC(C)(C)O)CC3
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 100 mg/mL (249.60 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4960 mL 12.4800 mL 24.9601 mL
    5 mM 0.4992 mL 2.4960 mL 4.9920 mL
    10 mM 0.2496 mL 1.2480 mL 2.4960 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.24 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.94%

    COA (249 KB) HNMR (217 KB) LCMS (126 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4960 mL 12.4800 mL 24.9601 mL 62.4002 mL
    5 mM 0.4992 mL 2.4960 mL 4.9920 mL 12.4800 mL
    10 mM 0.2496 mL 1.2480 mL 2.4960 mL 6.2400 mL
    15 mM 0.1664 mL 0.8320 mL 1.6640 mL 4.1600 mL
    20 mM 0.1248 mL 0.6240 mL 1.2480 mL 3.1200 mL
    25 mM 0.0998 mL 0.4992 mL 0.9984 mL 2.4960 mL
    30 mM 0.0832 mL 0.4160 mL 0.8320 mL 2.0800 mL
    40 mM 0.0624 mL 0.3120 mL 0.6240 mL 1.5600 mL
    50 mM 0.0499 mL 0.2496 mL 0.4992 mL 1.2480 mL
    60 mM 0.0416 mL 0.2080 mL 0.4160 mL 1.0400 mL
    80 mM 0.0312 mL 0.1560 mL 0.3120 mL 0.7800 mL
    100 mM 0.0250 mL 0.1248 mL 0.2496 mL 0.6240 mL
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    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Calcifediol Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Calcifediol19356-17-325-Hydroxy Vitamin D3VD/VDREndogenous MetaboliteVitamin DVitamin D receptorInhibitorinhibitorinhibit

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    製品名:
    Calcifediol
    製品番号:
    HY-32351
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