2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. STING Endogenous Metabolite
  4. Cyclic-di-GMP

Cyclic-di-GMP  (Synonyms: c-di-GMP; cyclic diguanylate; 5GP-5GP)

Cat. No.: HY-107780
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Cyclic-di-GMP is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP can be used in cancer research.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Cyclic-di-GMP disodium and Cyclic-di-GMP diammonium) that retains the same biological activity.

For research use only. We do not sell to patients.

CAS No. : 61093-23-0

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Other In-stock Forms of Cyclic-di-GMP:

Other Forms of Cyclic-di-GMP:

Cyclic-di-GMP Related Antibodies

Top Publications Citing Use of Products

    Cyclic-di-GMP purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) increased the protein expression of GSDMD-N, cleaved caspase-3, and phosphorylated-IRF3 (p-IRF3) in C57BL/6 N mice (Pan02-luc cells).

    Cyclic-di-GMP purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) combined with photothermal therapy (PTT) indicated the good biocompatibility in C57BL/6 N mice (Pan02-luc cells) by H&E staining of the major organs.

    Cyclic-di-GMP purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Mar 4;23(1):271.  [Abstract]

    Cyclic-di-GMP disodium (c-di-GMP; 200 μg/mL; 50 μL; intratumoural injection; day 0 and day 2) showed antitumour effect in C57BL/6 N mice (Pan02-luc cells).

    Cyclic-di-GMP purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    agarose gel electrophoresis analysis of DNA degradation assays. Nuclease activity assays were performed by incubating 0.5 μM VcCap5 with 20 ng/μl DNA in the presence of 0.5 μM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

    Cyclic-di-GMP purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    second messenger-dependent activation of VcCap5 DNA cleavage. Nuclease activity assays were performed by incubating 1 µM VcCap5 with 10 ng/µl DNA in the presence of 1 µM or 5 µM of various cyclic dinucleotides (Cyclic-di-GMP disodium; c-di-GMP).

    Cyclic-di-GMP purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2025 Dec 6;302(1):111021.  [Abstract]

    dose-response analysis of VcCap5 activity with increasing concentrations of cyclic dinucleotides. The nuclease assay mixture contained 1 µM VcCap5, 10 ng/µl DNA, and cyclic dinucleotide (Cyclic-di-GMP disodium; c-di-GMP) over a concentration range of 0.1 to 15 µM.

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    Human Endogenous Metabolite Microbial Metabolite

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    • Purity & Documentation

    • References

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    Description

    Cyclic-di-GMP is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP can be used in cancer research[1][2][3][4].

    IC50 & Target
    In Vitro

    Cyclic-di-GMP (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells[1].
    Cyclic-di-GMP (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
    Cyclic-di-GMP (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cyclic-di-GMP Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: H508 cells
    Concentration: 0.5-50 µM
    Incubation Time: 5 days
    Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

    Cell Viability Assay[2]

    Cell Line: Jurkat cells
    Concentration: 50 µM
    Incubation Time: 24 h
    Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

    Cell Cycle Analysis[2]

    Cell Line: Jurkat cells
    Concentration: 50 µM
    Incubation Time: 24 h
    Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
    In Vivo

    Cyclic-di-GMP (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6 (B6) mice (8- to 10-week-old)[3].
    Dosage: 100 µg/per
    Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
    Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
    Enhanced the anti-tumor activity of TriVax.
    Molecular Weight

    690.41

    Formula

    C20H24N10O14P2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)[C@H](OP(O)(OC[C@@H]4[C@H]([C@H]([C@H](N5C=NC6=C5N=C(N)NC6=O)O4)O)O7)=O)[C@H]3COP7(O)=O
    Structure Classification
    Initial Source

    bacterial species
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 25 mg/mL (36.21 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4484 mL 7.2421 mL 14.4841 mL
    5 mM 0.2897 mL 1.4484 mL 2.8968 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.18%

    SDS (252 KB)

    COA (254 KB) HNMR (196 KB) LCMS (774 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.4484 mL 7.2421 mL 14.4841 mL 36.2104 mL
    5 mM 0.2897 mL 1.4484 mL 2.8968 mL 7.2421 mL
    10 mM 0.1448 mL 0.7242 mL 1.4484 mL 3.6210 mL
    15 mM 0.0966 mL 0.4828 mL 0.9656 mL 2.4140 mL
    20 mM 0.0724 mL 0.3621 mL 0.7242 mL 1.8105 mL
    25 mM 0.0579 mL 0.2897 mL 0.5794 mL 1.4484 mL
    30 mM 0.0483 mL 0.2414 mL 0.4828 mL 1.2070 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Cyclic-di-GMP Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Cyclic-di-GMP61093-23-0c-di-GMP cyclic diguanylate 5GP-5GPSTINGEndogenous MetaboliteStimulator of Interferon GenesTMEM173MITAERISMPYSimmunotherapymalignant melanomaH508Jurkat cellscancerCD4Inhibitorinhibitorinhibit

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