2. TGF-beta/Smad Stem Cell/Wnt Cytoskeleton Cell Cycle/DNA Damage
  3. TGF-β Receptor Organoid Microtubule/Tubulin
  4. DMH-1

DMH-1 (GMP) is GMP grade DMH-1 (HY-12273). DMH-1 is a selective BMP inhibitor. DMH-1 upregulates the expression of SOX1. DMH-1 increases cardiomyocyte progenitor cells and promotes the differentiation of mouse embryonic stem cells into cardiomyocytes. DMH-1 induces the differentiation of hiPSC-derived neural progenitor cells into β3-tubulin-positive neurons.

For research use only. We do not sell to patients.

CAS No. : 1206711-16-1

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Customer Review

Based on 15 publication(s) in Google Scholar

Other Forms of DMH-1:

DMH-1 Related Antibodies

Top Publications Citing Use of Products

    DMH-1 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2024 Apr 16:e13646.  [Abstract]

    DMH-1 (5 mg/kg; i.p.; every other day for 3 weeks ). Hepatic expression levels of ID1, OPN, and SSTR2 in Tgm2CreERT2‐R26T/Gf/f mouse liver (n ≥ 4/group).

    DMH-1 purchased from MedChemExpress. Usage Cited in: J Genet Genomics. 2022 Dec;49(12):1101-1113.  [Abstract]

    The mitigation effect of Bmp inhibitor DMH1(5 mM; 2 days) on the hepatized intestine phenotype is dosage dependent. Left, representative WISH images; right, the intestine versus liver ratio of the fabp10a signals in cdx1bzju1/zju1. Embryos were treated with inhibitors starting from 36 hpf, respectively, and were subjected to WISH at 5 dpf with the fabp10a probe. Each spot represented one embryo.

    DMH-1 purchased from MedChemExpress. Usage Cited in: Technical University of Munich. 2015 Jun.

    Western blot analysis of MPC cells treated with DMH1. MPC cells are treated with DMH1 (3 μM or 5 μM) or with DMSO vehicle for 48 h. Proteins are then collected and probed for the expression of integrin β1, P-Smad1/5/8, P-S6 (1:500) and α-Tubulin is used as a loading control. Numbers below AKT and S6 represent the quantification of band intensities of P-AKT to AKT (OD P-AKT/ AKT) and P-S6 to S6 (OD PS6/S6) to determine the activity of the proteins.

    View All TGF-β Receptor Isoform Specific Products:

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    ALK1 ALK2 ALK3 ALK4 ALK5 ALK6 ALK7 TGFβR2 ACVR2A ACVR2B BMP

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DMH-1 (GMP) is GMP grade DMH-1 (HY-12273). DMH-1 is a selective BMP inhibitor. DMH-1 upregulates the expression of SOX1. DMH-1 increases cardiomyocyte progenitor cells and promotes the differentiation of mouse embryonic stem cells into cardiomyocytes. DMH-1 induces the differentiation of hiPSC-derived neural progenitor cells into β3-tubulin-positive neurons[1][2].

    In Vitro

    DMH-1 (0.5 μM) induces the formation of beating embryoid bodies (EBs), with an efficiency of approximately 20% on day 7, peaking at approximately 75% on day 9. Its efficiency at all evaluated time points is significantly higher than that of the DMSO control group[1].
    DMH-1 (0.5 μM) specifically upregulates the expression profile of canonical cardiogenic genes, including BryT, Mesp1, Isl1 and Nkx2.5, and does not significantly induce the expression of non-cardiomyocyte mesodermal, endodermal or ectodermal markers compared with the DMSO control group[1].
    DMH-1 (0.5 μM; 24-48 h) does not induce cell apoptosis[1].
    DMH-1 (0.5-10 μM; 7 days) increases the expression of SOX1 in hiPSCs in a concentration-dependent manner[2].
    DMH-1 (0.5 μM) induces hiPSC-derived neural progenitor cells to differentiate into β3-tubulin-positive neurons[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DMH-1 Related Antibodies

    Real Time qPCR[1]

    Cell Line: CGR8 mouse embryonic stem cell-derived EBs
    Concentration: 0.5 μM
    Incubation Time: from induction, assessed at Day 3, Day 4, Day 6, Day 8, Day 10
    Result: Significantly upregulated pre-mesoderm marker BryT (Day 3, Day 4), mesoderm lineage marker Mesp1 (Day 3, Day 4), second heart field marker Isl1 (Day 3, Day 4), and cardiomyocyte-specific marker Nkx2.5 (Day 6, Day 8, Day 10) relative to DMSO vehicle control.
    Did not specifically induce hematopoietic marker Gata1, multipotent progenitor marker Kdr/Flk-1, smooth muscle marker Myh11, endoderm markers Sox17 or FoxA2, or ectoderm marker Nestin relative to DMSO vehicle control.
    Induced a modest 2.3-fold increase in endocardial marker VE-CAM on Day 10 relative to DMSO.
    Molecular Weight

    380.44

    Formula

    C24H20N4O
    CAS No.
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    CC(C)OC1=CC=C(C2=CN3C(N=C2)=C(C4=CC=NC5=CC=CC=C45)C=N3)C=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 11.5 mg/mL (30.23 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6285 mL 13.1427 mL 26.2854 mL
    5 mM 0.5257 mL 2.6285 mL 5.2571 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1 mg/mL (2.63 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.95%

    SDS (419 KB)

    COA (255 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6285 mL 13.1427 mL 26.2854 mL 65.7134 mL
    5 mM 0.5257 mL 2.6285 mL 5.2571 mL 13.1427 mL
    10 mM 0.2629 mL 1.3143 mL 2.6285 mL 6.5713 mL
    15 mM 0.1752 mL 0.8762 mL 1.7524 mL 4.3809 mL
    20 mM 0.1314 mL 0.6571 mL 1.3143 mL 3.2857 mL
    25 mM 0.1051 mL 0.5257 mL 1.0514 mL 2.6285 mL
    30 mM 0.0876 mL 0.4381 mL 0.8762 mL 2.1904 mL
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    DMH-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DMH-11206711-16-1DMH1DMH 1TGF-β ReceptorOrganoidMicrotubule/TubulinTransforming growth factor beta receptorsInhibitorinhibitorinhibit

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