DMH-1 (GMP) is GMP grade DMH-1 (HY-12273). DMH-1 (GMP) is a selective BMP inhibitor. DMH-1 (GMP) upregulates the expression of SOX1. DMH-1 (GMP) increases cardiomyocyte progenitor cells and promotes the differentiation of mouse embryonic stem cells into cardiomyocytes. DMH-1 (GMP) induces the differentiation of hiPSC-derived neural progenitor cells into β3-tubulin-positive neurons.
For research use only. We do not sell to patients.
- CAS No.: 1206711-16-1
- Formula: C24H20N4O
- Molecular Weight:380.44
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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BMP |
DMH-1 (GMP) (0.5 μM) induces the formation of beating embryoid bodies (EBs), with an efficiency of approximately 20% on day 7, peaking at approximately 75% on day 9. Its efficiency at all evaluated time points is significantly higher than that of the DMSO control group[1].
DMH-1 (GMP) (0.5 μM) specifically upregulates the expression profile of canonical cardiogenic genes, including BryT, Mesp1, Isl1 and Nkx2.5, and does not significantly induce the expression of non-cardiomyocyte mesodermal, endodermal or ectodermal markers compared with the DMSO control group[1].
DMH-1 (GMP) (0.5 μM; 24-48 h) does not induce cell apoptosis[1].
DMH-1 (GMP) (0.5-10 μM; 7 days) increases the expression of SOX1 in hiPSCs in a concentration-dependent manner[2].
DMH-1 (GMP) (0.5 μM) induces hiPSC-derived neural progenitor cells to differentiate into β3-tubulin-positive neurons[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CGR8 mouse embryonic stem cell-derived EBs
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Concentration:0.5 μM
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Incubation Time:from induction, assessed at Day 3, Day 4, Day 6, Day 8, Day 10
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Result:Significantly upregulated pre-mesoderm marker BryT (Day 3, Day 4), mesoderm lineage marker Mesp1 (Day 3, Day 4), second heart field marker Isl1 (Day 3, Day 4), and cardiomyocyte-specific marker Nkx2.5 (Day 6, Day 8, Day 10) relative to DMSO vehicle control.
Did not specifically induce hematopoietic marker Gata1, multipotent progenitor marker Kdr/Flk-1, smooth muscle marker Myh11, endoderm markers Sox17 or FoxA2, or ectoderm marker Nestin relative to DMSO vehicle control.
Induced a modest 2.3-fold increase in endocardial marker VE-CAM on Day 10 relative to DMSO.
Chemical Information
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CAS No. 1206711-16-1
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Molecular Weight 380.44
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Formula C24H20N4O
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SMILES
CC(C)OC1=CC=C(C2=CN3C(N=C2)=C(C4=CC=NC5=CC=CC=C45)C=N3)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Ao A, et al. DMH1, a novel BMP small molecule inhibitor, increases cardiomyocyte progenitors and promotes cardiac differentiation in mouse embryonic stem cells. PLoS One. 2012;7(7):e41627. [Content Brief]
[2]. Neely MD, et al. DMH1, a highly selective small molecule BMP inhibitor promotes neurogenesis of hiPSCs: comparison of PAX6 and SOX1 expression during neural induction. ACS Chem Neurosci. 2012;3(6):482-491. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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