2. Vitamin D Related/Nuclear Receptor Apoptosis Autophagy
  3. Estrogen Receptor/ERR Apoptosis Autophagy
  4. DPN

DPN  (Synonyms: Diarylpropionitrile)

製品番号: HY-12452 純度: 99.66%
COA 取扱説明書 Technical Support

DPN (Diarylpropionitrile) is a non-steroidal estrogen receptor β (ERβ) selective ligand, with an EC50 of 0.85 nM. DPN has neuroprotective effects in a number of neurological diseases.

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CAS 番号 : 1428-67-7

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 46 在庫あり
Solution
10 mM * 1 mL in DMSO USD 46 在庫あり
Solid
5 mg $42 在庫あり
10 mg $66 在庫あり
25 mg $146 在庫あり
50 mg $264 在庫あり
100 mg $473 在庫あり
250 mg $878 在庫あり
500 mg $1430 在庫あり
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カスタマーレビュー

Based on 17 publication(s) in Google Scholar

Other Forms of DPN:

DPN 関連抗体

Top Publications Citing Use of Products

    DPN purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:818173.  [Abstract]

    Epidermal thickness decreased after PHTPP treatment but increased after DPN (12 mg/kg, s.c.) injection by H&E-stained.

    DPN purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 May 19;13:818173.  [Abstract]

    γδ T+ cells in the draining lymph nodes from female and male mice, OVX and sham operated mice, DPN (12 mg/kg, s.c.) treated mice with and without mannan application.

    DPN purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Jul 22;10(8):565.  [Abstract]

    Cells were treated with DPN (10 nM), the cytoplasm distribution of endogenous LC3 punctate structures was observed by confocal microscopy in SH-SY5Y cells.

    DPN purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Jul 22;10(8):565.  [Abstract]

    Cells were treated with DPN (10 nM), CQ (10 μM), and DPN plus CQ for 12 h. Cell lysates were analyzed by immunoblotting for LC3-II, SQSTM1, and β-actin protein expression.

    DPN purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Jul 22;10(8):565.  [Abstract]

    Cells were treated with DPN (10 nM) or Aβ1–42 (5 μM) for 12 h and then added CQ (10 μM) for another 12 h. The MTT assay was used to test cell viability.

    Estrogen Receptor/ERR アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    DPN (Diarylpropionitrile) is a non-steroidal estrogen receptor β (ERβ) selective ligand, with an EC50 of 0.85 nM. DPN has neuroprotective effects in a number of neurological diseases[1][2].

    IC50 & Target[1]

    ERβ

    0.85 nM (EC50)

    Cellular Effect
    Cell Line Type Value Description References
    HEC-1 EC50
    0.85 nM
    Compound: rac-5
    Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay
    Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay
    		[PMID: 19286283]
    HEC-1 EC50
    3.3 nM
    Compound: 1, rac-DPN
    Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay
    Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay
    		[PMID: 22122563]
    HEC-1 EC50
    304 nM
    Compound: 1, rac-DPN
    Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay
    Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay
    		[PMID: 22122563]
    HEC-1 EC50
    66 nM
    Compound: rac-5
    Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay
    Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay
    		[PMID: 19286283]
    U2OS EC50
    208 nM
    Compound: 1, rac-DPN
    Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay
    Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay
    		[PMID: 22122563]
    U2OS EC50
    3.7 nM
    Compound: 1, rac-DPN
    Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay
    Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay
    		[PMID: 22122563]
    体外実験

    DPN has a 70-fold ERα relative binding affinity selectivity, and it is a full ERα agonist with a 78-fold ERα potency selectivity (EC50=0.85 nM for ERβ; EC50=66 nM for ERα)[1].
    DPN (10 nM) prevents morphological alterations from Aβ1-42 (10 μM)-induced toxicity in cultured cortical neurons[2].
    DPN (0.1-100 nM) decreases ROS levels in a non-dose response manner[2].
    DPN (0.1-100 nM) significantly reduces Aβ1-42-stimulated expression of Bax in a non-dose dependent manner[2].
    DPN (0.1-100 nM) reduces activated IL-1 levels induced by Aβ1-42 treatment on cultured cortical neurons[2].
    DPN (0.1-100 nM) suppresses the Aβ1-42-upregulated phosphorylation of JNK and p38[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DPN 関連抗体
    体内実験

    DPN (10 μg; s.c.; daily; for 11 days) increases swimming and decreases immobility in the FST, and increases TPH protein expression in the dorsal raphe nucleus (DR) in rat model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult Sprague-Dawley female rats (220-250 g), ovariectomized animal models[3]
    Dosage: 10 μg/rat
    Administration: Subcutaneous injections, daily, for 11 days
    Result: Increased swimming and decreased immobility in the FST.
    分子量

    239.27

    分子式

    C15H13NO2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC1=CC=C(CC(C#N)C2=CC=C(O)C=C2)C=C1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (417.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1793 mL 20.8965 mL 41.7931 mL
    5 mM 0.8359 mL 4.1793 mL 8.3586 mL
    10 mM 0.4179 mL 2.0897 mL 4.1793 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.66%

    COA (245 KB) LCMS (403 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.1793 mL 20.8965 mL 41.7931 mL 104.4827 mL
    5 mM 0.8359 mL 4.1793 mL 8.3586 mL 20.8965 mL
    10 mM 0.4179 mL 2.0897 mL 4.1793 mL 10.4483 mL
    15 mM 0.2786 mL 1.3931 mL 2.7862 mL 6.9655 mL
    20 mM 0.2090 mL 1.0448 mL 2.0897 mL 5.2241 mL
    25 mM 0.1672 mL 0.8359 mL 1.6717 mL 4.1793 mL
    30 mM 0.1393 mL 0.6966 mL 1.3931 mL 3.4828 mL
    40 mM 0.1045 mL 0.5224 mL 1.0448 mL 2.6121 mL
    50 mM 0.0836 mL 0.4179 mL 0.8359 mL 2.0897 mL
    60 mM 0.0697 mL 0.3483 mL 0.6966 mL 1.7414 mL
    80 mM 0.0522 mL 0.2612 mL 0.5224 mL 1.3060 mL
    100 mM 0.0418 mL 0.2090 mL 0.4179 mL 1.0448 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    DPN Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DPN1428-67-7DiarylpropionitrileEstrogen Receptor/ERRApoptosisAutophagyestrogenreceptorbetaAlzheimer'sdiseaseamyloidpeptideantioxidantapoptosisanti-inflammationneuroprotectionanxiety-likebehaviorsInhibitorinhibitorinhibit

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    製品名:
    DPN
    製品番号:
    HY-12452
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