1. Membrane Transporter/Ion Channel
  2. SGLT
  3. Dapagliflozin ((2S)-1,2-propanediol, hydrate)

Dapagliflozin ((2S)-1,2-propanediol, hydrate)  (Synonyms: BMS-512148 (2S)-1,2-propanediol, hydrate)

Cat. No.: HY-10450A Purity: 99.95%
COA Handling Instructions

Dapagliflozin ((2S)-1,2-propanediol, hydrate) is the S-enantiomer of Dapagliflozin 1,2-propanediol, hydrate. Dapagliflozin ((2S)-1,2-propanediol, hydrate), a new type of agent used to treat diabetes mellitus (DM), is a competitive sodium/glucose cotransporter 2 (SGLT2) inhibitor, which results in excretion of glucose into the urine. Dapagliflozin ((2S)-1,2-propanediol, hydrate) induces HIF1 expression and attenuates renal IR injury.

For research use only. We do not sell to patients.

Dapagliflozin ((2S)-1,2-propanediol, hydrate) Chemical Structure

Dapagliflozin ((2S)-1,2-propanediol, hydrate) Chemical Structure

CAS No. : 960404-48-2

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 61 In-stock
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Solid
5 mg USD 55 In-stock
10 mg USD 77 In-stock
50 mg USD 121 In-stock
100 mg USD 154 In-stock
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Customer Review

Based on 37 publication(s) in Google Scholar

Other Forms of Dapagliflozin ((2S)-1,2-propanediol, hydrate):

Top Publications Citing Use of Products

31 Publications Citing Use of MCE Dapagliflozin ((2S)-1,2-propanediol, hydrate)

Cell Viability Assay
IF
WB

    Dapagliflozin ((2S)-1,2-propanediol, hydrate) purchased from MedChemExpress. Usage Cited in: Life Sci. 2023 Apr 4;121671.  [Abstract]

    Dapagliflozin (DAPA; 10 μM; 24 hours) significantly decreases the expression of YAP/TAZ and increases the ratios of p-YAP/YAP and pTAZ/TAZ under high glucose conditions in HK-2 cells.

    Dapagliflozin ((2S)-1,2-propanediol, hydrate) purchased from MedChemExpress. Usage Cited in: Life Sci. 2023 Apr 4;121671.  [Abstract]

    Dapagliflozin (DAPA; 10 μM; 24 hours) significantly reduces the high glucose-induced increase in YAP/TAZ expression in HK-2 cells.

    Dapagliflozin ((2S)-1,2-propanediol, hydrate) purchased from MedChemExpress. Usage Cited in: Life Sci. 2023 Apr 4;121671.  [Abstract]

    Dapagliflozin (DAPA; 10, 20 μM; 24 h) significantly reverses the decrease in cell viability caused by high glucose in HK-2 cells.

    Dapagliflozin ((2S)-1,2-propanediol, hydrate) purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022, 86: 104342.

    Dapagliflozin increases the LC3-II to LC3-I expression ratio in the liver.

    Dapagliflozin ((2S)-1,2-propanediol, hydrate) purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2018 Jun;152:45-59.  [Abstract]

    Effects of Dapagliflozin and Empagliflozin on p62 expression in LPS-untreated and LPS-treated RAW264.7 cells.

    Dapagliflozin ((2S)-1,2-propanediol, hydrate) purchased from MedChemExpress. Usage Cited in: Cell Physiol Biochem. 2018;45(5):1747-1758.  [Abstract]

    The expression of IRS1 significantly decreases. The phosphorylation levels of Akt1, Akt2, and S6 are significantly reduced in livers of db/db mice.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Dapagliflozin ((2S)-1,2-propanediol, hydrate) is the S-enantiomer of Dapagliflozin 1,2-propanediol, hydrate. Dapagliflozin ((2S)-1,2-propanediol, hydrate), a new type of agent used to treat diabetes mellitus (DM), is a competitive sodium/glucose cotransporter 2 (SGLT2) inhibitor, which results in excretion of glucose into the urine[1]. Dapagliflozin ((2S)-1,2-propanediol, hydrate) induces HIF1 expression and attenuates renal IR injury[2].

    IC50 & Target

    SGLT2

     

    In Vitro

    Dapagliflozin ((2S)-1,2-propanediol, hydrate) (0-10 μM; 24 hours) significantly increases the cell survival in hypoxic HK2 cell in a dose-dependent manner[2].
    Dapagliflozin ((2S)-1,2-propanediol, hydrate) (0-10 μM; 2 hours) increases the HIF1 expression, increases AMPK and EKR phosphorylation in hypoxic HK2 cells, but shows no effect on the phosphorylation of AMPK and ERK in normoxic HK2 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: Hypoxic HK2 cell
    Concentration: 0 μM, 1 μM, 2 μM, 5 μM, 10 μM
    Incubation Time: 24 hours
    Result: Improved the cell viability in a dose-dependent manner compared with control cells.

    Western Blot Analysis[1]

    Cell Line: Hypoxic HK2 cell, Normoxic HK2 cells
    Concentration: 0 μM, 1 μM, 2 μM, 5 μM, 10 μM
    Incubation Time: 24 hours
    Result: Induced HIF1 expression in hypoxic and normoxic HK2 cells.
    Clinical Trial
    Molecular Weight

    502.98

    Formula

    C24H35ClO9

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1[C@H](C2=CC(CC3=CC=C(OCC)C=C3)=C(Cl)C=C2)O[C@H](CO)[C@@H](O)[C@@H]1O.C[C@H](O)CO.O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture

    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (198.82 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 2.38 mg/mL (4.73 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9882 mL 9.9408 mL 19.8815 mL
    5 mM 0.3976 mL 1.9882 mL 3.9763 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.97 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.97 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 2.5 mg/mL (4.97 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.99%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.9882 mL 9.9408 mL 19.8815 mL 49.7038 mL
    DMSO 5 mM 0.3976 mL 1.9882 mL 3.9763 mL 9.9408 mL
    10 mM 0.1988 mL 0.9941 mL 1.9882 mL 4.9704 mL
    15 mM 0.1325 mL 0.6627 mL 1.3254 mL 3.3136 mL
    20 mM 0.0994 mL 0.4970 mL 0.9941 mL 2.4852 mL
    25 mM 0.0795 mL 0.3976 mL 0.7953 mL 1.9882 mL
    30 mM 0.0663 mL 0.3314 mL 0.6627 mL 1.6568 mL
    40 mM 0.0497 mL 0.2485 mL 0.4970 mL 1.2426 mL
    50 mM 0.0398 mL 0.1988 mL 0.3976 mL 0.9941 mL
    60 mM 0.0331 mL 0.1657 mL 0.3314 mL 0.8284 mL
    80 mM 0.0249 mL 0.1243 mL 0.2485 mL 0.6213 mL
    100 mM 0.0199 mL 0.0994 mL 0.1988 mL 0.4970 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Dapagliflozin ((2S)-1,2-propanediol, hydrate) Related Classifications

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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