1. Cell Cycle/DNA Damage
    Autophagy
    Antibody-drug Conjugate/ADC Related
  2. Topoisomerase
    Autophagy
    ADC Cytotoxin
  3. Daunorubicin Hydrochloride

Daunorubicin Hydrochloride (Synonyms: RP 13057 (Hydrochloride); Daunomycin (Hydrochloride); Rubidomycin (Hydrochloride))

Cat. No.: HY-13062 Purity: 99.27%
Handling Instructions

Daunorubicin hydrochloride (RP 13057 Hydrochloride, Daunomycin Hydrochloride, Rubidomycin Hydrochloride) is a topoisomerase II inhibitor with potent antineoplastic activities.

For research use only. We do not sell to patients.

Daunorubicin Hydrochloride Chemical Structure

Daunorubicin Hydrochloride Chemical Structure

CAS No. : 23541-50-6

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in Water USD 79 In-stock
Estimated Time of Arrival: December 31
10 mg USD 72 In-stock
Estimated Time of Arrival: December 31
50 mg USD 180 In-stock
Estimated Time of Arrival: December 31
100 mg USD 300 In-stock
Estimated Time of Arrival: December 31
200 mg USD 420 In-stock
Estimated Time of Arrival: December 31
500 mg USD 540 In-stock
Estimated Time of Arrival: December 31
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

Customer Review

Other Forms of Daunorubicin Hydrochloride:

Top Publications Citing Use of Products

View All Topoisomerase Isoform Specific Products:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Daunorubicin hydrochloride (RP 13057 Hydrochloride, Daunomycin Hydrochloride, Rubidomycin Hydrochloride) is a topoisomerase II inhibitor with potent antineoplastic activities.

IC50 & Target[1]

Topoisomerase II

 

In Vitro

The mean IC50 value is 0.04 μM for Daunorubicin (Dnr) in Molt-4 cells. Daunorubicin belongs to the anthracyclines, a group of cytotoxic chemotherapeutics. The cytotoxic effects of anthracyclines are caused by DNA intercalation and the ability to interfere with DNA transcription and replication by inhibiting Topoisomerase II as well as by producing reactive oxygen species[2] Daunorubicin inhibits of both DNA and RNA syntheses in HeLa cells over a concentration range of 0.2 through 2 μM. The IC50 value is 0.4 μM for Daunorubicin (Dnr) in human pancreatic cell line L3.6[3].

In Vivo

Urinary protein excretion, serum creatinine, and blood urea nitrogen (BUN) level are significantly increased in group Daunorubicin (3 mg/kg, i.v.) compared with those in group Control. Administration of Daunorubicin (DNR) causes a significant increase in malondialdehyde (MDA) level in renal tissue compared with that in the control group[4].

Clinical Trial
Storage

4°C, protect from light

Solvent & Solubility
In Vitro: 

H2O : ≥ 34 mg/mL (60.29 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7731 mL 8.8656 mL 17.7311 mL
5 mM 0.3546 mL 1.7731 mL 3.5462 mL
10 mM 0.1773 mL 0.8866 mL 1.7731 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[2]

The chemosensitivity to Daunorubicin is assessed using the MTT assay. In brief, the 96 well plates are set up with cells at the initial density of 2×105 cells/mL and are incubated at 37°C for 72 h in an atmosphere of 5% CO2 in the absence and presence of nine different concentrations of Daunorubicin (Dnr) or Dox ranging from 1.90 to 0.007 μM in triplicate. After incubation, 10 μL of MTT solution (5 mg/mL tetrazolium salt) is added to each well and the plates are incubated for a further 4 h at 37°C. The formazan salt crystals are dissolved by adding 100 μL 10% SDS in 10 mM HCl solution and incubating over night at 37°C. The absorbance is measured at 540 nm with a reference at 650 nm by a 96-well enzyme-linked immunosorbent assay (ELISA) plate reader. Chemosensitivity is expressed as the IC50, which is the concentration of drug causing 50% cell survival compare to control cells grown without drug. Calculations are carried out using Microsoft Excel[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[4]

Rat[4]
Eight-week-old male Sprague-Dawley rats are used. The animals are quarantined and acclimatized for the additional 2 weeks prior to the initiation of the experiments. On day 0, each animal receives a single intravenous injection of Daunorubicin at a dose of 3 mg/kg (i.v.). Daunorubicin is administered in three equal injections at 48 h intervals for a period of one week to achieve an accumulative dose of 9 mg/kg, which is well documented to produce cardiotoxicity and nephrotoxicity. Age-matched rats are injected with corresponding volumes of 0.9% NaCl and used as a control (group Control;n=5). Twenty-two DNR-treated rats are randomly divided into two groups and received oral administration of Telmisartan (10 mg/kg/day; group Daunorubicin+Telmisartan; n=10) or vehicle (group Daunorubicin; n=12). The dose of Telmisartan is chosen on the basis of a previous report. Administration of Telmisartan is started on the same day as Daunorubicin administration and continued for 5 additional weeks after cessation of Daunorubicin administration (6 weeks total period). This duration of study is chosen on the basis of previous reports. On day 41, rats are placed individually in metabolic cages for 24-h urine collections for the measurement of protein concentrations and body weight (BW) is measured. After the end of the study period (6 weeks), rats are sacrificed and kidney tissue is harvested for semi-quantitative immunoblotting and immunohistochemical studies.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

563.98

Formula

C₂₇H₃₀ClNO₁₀

CAS No.

23541-50-6

SMILES

O=C(C(C(OC)=CC=C1)=C1C2=O)C3=C2C(O)=C(C[[email protected]@](O)(C(C)=O)C[[email protected]@H]4O[[email protected]@]5([H])C[[email protected]](N)[[email protected]](O)[[email protected]](C)O5)C4=C3O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere

Purity: 99.27%

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Daunorubicin Hydrochloride
Cat. No.:
HY-13062
Quantity:

Daunorubicin Hydrochloride

Cat. No.: HY-13062