1. Cell Cycle/DNA Damage
    Antibody-drug Conjugate/ADC Related
    Autophagy
    Anti-infection
    Apoptosis
  2. Topoisomerase
    DNA/RNA Synthesis
    ADC Cytotoxin
    Autophagy
    Bacterial
    Antibiotic
    Apoptosis
  3. Daunorubicin

Daunorubicin  (Synonyms: Daunomycin; RP 13057; Rubidomycin)

Cat. No.: HY-13062A
Handling Instructions

Daunorubicin (Daunomycin) is a topoisomerase II inhibitor with potent anti-tumor activity. Daunorubicin inhibits DNA and RNA synthesis. Daunorubicin is a cytotoxin that inhibits cancer cell viability and induces apoptosis and necrosis. Daunorubicin is also an anthracycline antibiotic. Daunorubicin can be used in the research of infection and variety of cancers, including leukemia, non-Hodgkin lymphomas, Ewing's sarcoma, Wilms' tumor.

For research use only. We do not sell to patients.

Daunorubicin Chemical Structure

Daunorubicin Chemical Structure

CAS No. : 20830-81-3

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Description

Daunorubicin (Daunomycin) is a topoisomerase II inhibitor with potent anti-tumor activity. Daunorubicin inhibits DNA and RNA synthesis. Daunorubicin is a cytotoxin that inhibits cancer cell viability and induces apoptosis and necrosis. Daunorubicin is also an anthracycline antibiotic. Daunorubicin can be used in the research of infection and variety of cancers, including leukemia, non-Hodgkin lymphomas, Ewing's sarcoma, Wilms' tumor[1][2][4][5].

IC50 & Target[1][2]

Topoisomerase II

 

Daunorubicins/Doxorubicins

 

In Vitro

Daunorubicin (0-256 μg/mL, 30 min) inhibits DNA and RNA synthesis in sensitive and resistant Ehrlich ascites tumor cells[2].
Daunorubicin (7 nM-1.9 μM, 72 h) shows chemosensitivity in Molt-4 cells and L3.6 cells[3][4].
Daunorubicin (0.4 μM, 48 h) induces apoptotic and necrosis in L3.6 cells[4].
Daunorubicin (0.4 μM, 120 min) induces ROS generation in L3.6 cells[4].
Daunorubicin (2 μM, 24 h) induces autophagy in K562 cells (myeloid cell line)[6].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3][4]

Cell Line: Molt-4 cells (a human T-lymphoblastic leukemia cell line), L3.6 cells (metastatic human pancreatic cell line)
Concentration: 7 nM-1.9 μM
Incubation Time: 72 h
Result: Inhibited cell viability with IC50 values of 40 nM (Molt-4) and 400 nM (L3.6).

Apoptosis Analysis[4]

Cell Line: L3.6 cells
Concentration: 0.4 μM
Incubation Time: 24 h, 48 h
Result: Induced necrosis without apoptosis at 24 h, induced both an apoptotic and extensive necrotic response at 48 h.

Western Blot Analysis[6]

Cell Line: K562 cells
Concentration: 2 μM
Incubation Time: 24 h
Result: Enabled the switch of LC3-I into LC3-II, accompanied with a significant increased expression level of LC3.
In Vivo

Daunorubicin (intravenous injection, 3 mg/kg, three times at 48 h intervals.) produces cardiotoxicity and nephrotoxicity in rats[5].
Daunorubicin (intraperitoneal injection, 10 mg/kg) induces sister chromatid exchanges in mice[7].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats[5]
Dosage: 3 mg/kg
Administration: Intravenous injection, three times at 48 h intervals.
Result: Caused a significant increase in MDA (malondialdehyde) level in renal tissue, accompanied by a significant reduction in total GPx activity.
Increased urinary protein excretion, serum creatinine, and BUN level.
Clinical Trial
Molecular Weight

527.52

Formula

C27H29NO10

CAS No.
SMILES

O=C(C(C(OC)=CC=C1)=C1C2=O)C3=C2C(O)=C(C[[email protected]@](O)(C(C)=O)C[[email protected]@H]4O[[email protected]@]5([H])C[[email protected]](N)[[email protected]](O)[[email protected]](C)O5)C4=C3O

Structure Classification
Source

Streptomyces peucetius and S. peucetius subsp. caesius

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Daunorubicin
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