1. GPCR/G Protein
    Metabolic Enzyme/Protease
  2. G protein-coupled Bile Acid Receptor 1
    Endogenous Metabolite
  3. Deoxycholic acid

Deoxycholic acid (Synonyms: Cholanoic Acid; Desoxycholic acid)

Cat. No.: HY-N0593 Purity: 99.89%
Handling Instructions

Deoxycholic acid (cholanoic acid), a bile acid, is a by-product of intestinal metabolism, that activates the G protein-coupled bile acid receptorTGR5.

For research use only. We do not sell to patients.

Deoxycholic acid Chemical Structure

Deoxycholic acid Chemical Structure

CAS No. : 83-44-3

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Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
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ready for reconstitution
USD 73 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 66 In-stock
Estimated Time of Arrival: December 31
500 mg USD 92 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Deoxycholic acid:

Top Publications Citing Use of Products

    Deoxycholic acid purchased from MCE. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.

    The expression of CDX2, KLF4, HNF4α, and cadherin 17 is increased upon stimulation with DCA in a dose-dependent manner.

    Deoxycholic acid purchased from MCE. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.

    The expression of CDX2, KLF4, HNF4α, and cadherin 17 is increased upon stimulation with DCA in a dose-dependent manner.

    Deoxycholic acid purchased from MCE. Usage Cited in: Cancer Cell Int. 2019 Jan 31;19:24.

    Western blotting results indicated that the inhibition of SOX2 in bile acid-treated AGS cells is partially rescued in miR-21 knockdown AGS cells.
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    Description

    Deoxycholic acid (cholanoic acid), a bile acid, is a by-product of intestinal metabolism, that activates the G protein-coupled bile acid receptorTGR5[1][2].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    In Vitro

    Deoxycholic acid (DCA) (100 μM) induces the production of gastric cancer cell line MGC803 resistant to acidified bile acids and enhances their survival and proliferation activity under bile acid stress[2].
    Deoxycholic acid (DCA) (100 μM)-induced resistant cells shows altered morphology, significantly elevated telomerase activity, better cell viability and reduces apoptosis compared to normal MGC803 cells[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    392.57

    Formula

    C24H40O4

    CAS No.
    SMILES
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (254.73 mM)

    H2O : < 0.1 mg/mL (ultrasonic;warming;heat to 80°C) (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5473 mL 12.7366 mL 25.4732 mL
    5 mM 0.5095 mL 2.5473 mL 5.0946 mL
    10 mM 0.2547 mL 1.2737 mL 2.5473 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 2.5 mg/mL (6.37 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.89%

    References
    Cell Assay
    [2]

    MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented with 10% fetal calf serum and 100 U/mL Penicillin and 100 mg/mL Streptomycin. To generate MGC803-resistant cells, the pH value of the MGC803 culture medium is adjusted to the experimental conditions using the hydrochloric acid (A). The bile acids GCDA and Deoxycholic acid are diluted to optimal working concentrations of 100 μM (B) with culture medium, and the overall pH (A+B) is adjusted to pH 5.5, simulating the gastric environment. Initially, MGC803 cells are chronically exposed to acidified medium with bile acids (A+B) for 10 min every 24 h. The experimental time and conditions are optimized in our preliminary experiments, which show that 10 min is enough and does not result in cell damage. This procedure is repeated and it takes 60 weeks for the MGC803 cells to survive and proliferate under the exposure of A+B for 120 min. Control untreated cells are cultured in neutral RPMI medium at pH 7.4 in parallel to the resistant cells for 60 weeks. The morphological changes in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and 60 weeks[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Product Name:
    Deoxycholic acid
    Cat. No.:
    HY-N0593
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