2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Tetrahydrofolic acid

テトラヒドロ葉酸  (Synonyms: Tetrahydrofolic acid; L-テトラヒドロ葉酸; L-5,6,7,8-Tetrahydrofolic acid; L-Tetrahydrofolic acid)

製品番号: HY-14520 純度: 96.77%
COA 取扱説明書 Technical Support

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

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CAS 番号 : 135-16-0

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カスタマーレビュー

Based on 6 publication(s) in Google Scholar

Other Forms of Tetrahydrofolic acid:

テトラヒドロ葉酸 関連抗体

Top Publications Citing Use of Products

    Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233.  [Abstract]

    Micehepatocytes were pretreated with indicated concentrations of L-methionine (L-Met) or tetrahydrofolic acid (THF) for 2 h before adding 200 μM DCA for 4 h.

    Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Dec 19:139041.  [Abstract]

    The effect of Tetrahydrofolic acid (25-75 μM) on the aggregation of αS and Aβ was monitored by ThT fluorescence.

    Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Dec 19:139041.  [Abstract]

    αS, Aβ40, and Aβ42 were incubated for 340 h, 110 h, and 20 h, respectively, in the absence or presence of a specified proportion of Tetrahydrofolic acid for AFM analysis. Scale bars = 1 μm.

    Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Dec 19:139041.  [Abstract]

    The effect of 50 μM αS, 25 μM Aβ40 and 25 μM Aβ42 aggregates on cell viability without or with different concentrations of Tetrahydrofolic acid (25-100 μM; 48 h) was determined by MTT assay.

    Tetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Dec 19:139041.  [Abstract]

    Tetrahydrofolic acid (75-100 μM; 72 h) accelerates amyloid aggregation in C. elegans.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

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    製品説明

    Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid[1].

    IC50 & Target[1]

    Human Endogenous Metabolite

     

    体外実験

    Tetrahydrofolic acid (0-200 μM; 3 days; Adh5-/- DT40 cells) exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks[1].
    Tetrahydrofolic acid (0-100 μM; 16 hours; Adh5-/- DT40 cells) treatment strongly promots FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner[1].
    Tetrahydrofolic acid exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    テトラヒドロ葉酸 関連抗体

    Cell Viability Assay[1]

    Cell Line: Adh5-/- DT40 cells
    Concentration: 0-200 µM
    Incubation Time: 3 days
    Result: Viability of Adh5-/- DT40 cells rapidly dropped.

    Western Blot Analysis[1]

    Cell Line: Adh5-/- DT40 cells
    Concentration: 0-200 µM
    Incubation Time: 16 hours
    Result: Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner.
    体内実験

    Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5-/- mice) treatment perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid could be mutagenic and genotoxic to bone marrow cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adh5-/- mice[1]
    Dosage: 62.5 mg/kg
    Administration: Intraperitoneal injection; daily
    Result: Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).
    分子量

    445.43

    分子式

    C19H23N7O6
    CAS 番号
    Appearance

    Solid

    Color

    Light brown to brown

    SMILES

    NC(NC1=C2NC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)CN1)=NC2=O
    Structure Classification
    Initial Source
    輸送条件

    Shipping with dry ice.
    保管条件

    -80°C, protect from light, stored under nitrogen

    *The compound is unstable in solutions, freshly prepared is recommended.
    溶剤 & 溶解度
    体外: 

    DMSO : 116.67 mg/mL (261.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2450 mL 11.2251 mL 22.4502 mL
    5 mM 0.4490 mL 2.2450 mL 4.4900 mL
    10 mM 0.2245 mL 1.1225 mL 2.2450 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    純度とドキュメンテーション

    純度: 96.77%

    COA (247 KB) HNMR (265 KB) RP-HPLC (253 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2450 mL 11.2251 mL 22.4502 mL 56.1255 mL
    5 mM 0.4490 mL 2.2450 mL 4.4900 mL 11.2251 mL
    10 mM 0.2245 mL 1.1225 mL 2.2450 mL 5.6126 mL
    15 mM 0.1497 mL 0.7483 mL 1.4967 mL 3.7417 mL
    20 mM 0.1123 mL 0.5613 mL 1.1225 mL 2.8063 mL
    25 mM 0.0898 mL 0.4490 mL 0.8980 mL 2.2450 mL
    30 mM 0.0748 mL 0.3742 mL 0.7483 mL 1.8709 mL
    40 mM 0.0561 mL 0.2806 mL 0.5613 mL 1.4031 mL
    50 mM 0.0449 mL 0.2245 mL 0.4490 mL 1.1225 mL
    60 mM 0.0374 mL 0.1871 mL 0.3742 mL 0.9354 mL
    80 mM 0.0281 mL 0.1403 mL 0.2806 mL 0.7016 mL
    100 mM 0.0225 mL 0.1123 mL 0.2245 mL 0.5613 mL
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    Tetrahydrofolic acid Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tetrahydrofolic acid135-16-0L-5,6,7,8-Tetrahydrofolic acid L-Tetrahydrofolic acidEndogenous MetaboliteFolatederivativewater-solublevitamin5,10-methylenetetrahydrofolatehematopoiesisFANCD2one-carbonDNAaminonucleiclipidsInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-14520
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