2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. ML349

ML349 is a potent and specific acyl protein thioesterase 2(APT2)/lysophospholipase 2 (LYPLA2) inhibitor with a Ki of 120 nM. ML349 is also an inhibitor of LYPLA2 with an IC50 of 144 nM.

For research use only. We do not sell to patients.

CAS No. : 890819-86-0

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10 mM * 1 mL in DMSO
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of ML349:

ML349 Related Antibodies

Top Publications Citing Use of Products

    ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109.  [Abstract]

    PEMs were treated with ML349 (0-15 μM) at the indicated concentrations for 12 h. Cell lysates were collected for ABE assay and immunoblot analysis. This was repeated n = 3 independent times with similar results.

    ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109.  [Abstract]

    HEK293T cells were transfected with a luciferase reporter for IFN-β-luc and FLAG-TBK1 for 24 h, followed by treatment with ML349 (0-12 h) at different time points. Cell lysates were collected for luciferase reporter assays (n = 3 biological replicates).

    ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109.  [Abstract]

    PEMs were treated with ML349 (10 μM) for 12 h, then stimulated with gDNA at the indicated time points. Cell lysates were collected for immunoblot.

    ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109.  [Abstract]

    PEMs were treated with ML349 (10 μM) for 12 h, then stimulated with gDNA at the indicated time points. Cell lysates were collected for RT-qPCR analysis.

    ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109.  [Abstract]

    PEMs were treated with ML349 (0-15 μM) at indicated concentrations for 12 h. Cell lysates were collected for immunoblot analysis. This was repeated n = 3 independent times with similar results.

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    • Protocol

    • Purity & Documentation

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    Description

    ML349 is a potent and specific acyl protein thioesterase 2(APT2)/lysophospholipase 2 (LYPLA2) inhibitor with a Ki of 120 nM. ML349 is also an inhibitor of LYPLA2 with an IC50 of 144 nM[1][2].

    IC50 & Target

    Ki: 120 nM (APT-2)[1]
    IC50: 144 nM (LYPLA2)[2]
    Cellular Effect
    Cell Line Type Value Description References
    HEK-293T IC50
    1.1 μM
    Compound: ML349
    Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
    Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
    		[PMID: 28197315]
    HEK-293T IC50
    > 10 μM
    Compound: ML349
    Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged PDXK expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
    Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged PDXK expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
    		[PMID: 28197315]
    In Vitro

    ML349 is an inhibitor of acyl protein thioesterase 1 and 2 (APT-1 and APT-2) with Kis of >10000 and 120±20 nM, respectively[1].
    ML349 is also an inhibitor of both LYPLA1 and LYPLA2 with IC50s of >3000 and 144 nM, respectivley[2].
    ML348 and ML349 do not decrease cell viability, but they lead to a slight activation of AKT in NRAS mutant cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ML349 Related Antibodies
    Molecular Weight

    454.56

    Formula

    C23H22N2O4S2
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    COC1=CC=C(N2CCN(C(C3=CC4=C(C(C=CC=C5)=C5S(C4)(=O)=O)S3)=O)CC2)C=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (55.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1999 mL 10.9996 mL 21.9993 mL
    5 mM 0.4400 mL 2.1999 mL 4.3999 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2 mg/mL (4.40 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.47%

    SDS (251 KB)

    COA (249 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [3]

    Cells are plated in 96-well plates with a density of 4000 to 8000 cells per well and incubated for 24 h at 37°C with 5% CO2. Then cells are treated with increasing drug (including ML349) concentrations and their combinations. Cell viability is measured with the cell viability assay according to the manufacturer's protocol[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1999 mL 10.9996 mL 21.9993 mL 54.9982 mL
    5 mM 0.4400 mL 2.1999 mL 4.3999 mL 10.9996 mL
    10 mM 0.2200 mL 1.1000 mL 2.1999 mL 5.4998 mL
    15 mM 0.1467 mL 0.7333 mL 1.4666 mL 3.6665 mL
    20 mM 0.1100 mL 0.5500 mL 1.1000 mL 2.7499 mL
    25 mM 0.0880 mL 0.4400 mL 0.8800 mL 2.1999 mL
    30 mM 0.0733 mL 0.3667 mL 0.7333 mL 1.8333 mL
    40 mM 0.0550 mL 0.2750 mL 0.5500 mL 1.3750 mL
    50 mM 0.0440 mL 0.2200 mL 0.4400 mL 1.1000 mL
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    ML349 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ML349890819-86-0ML 349ML-349PhospholipaseInhibitorinhibitorinhibit

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