5'-N-Ethylcarboxamidoadenosine (Synonyms: NECA)

Name: 5'-N-Ethylcarboxamidoadenosine
Brand: MedChemExpress
SKU: HY-103173
Rating: 5.0 (7 reviews)

Art. -Nr.: HY-103173 Reinheit: 99.09%: Data Sheet
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5'-N-Ethylcarboxamidoadenosine (NECA) is a nonselective adenosine receptor agonist.

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CAS. Nr. : 35920-39-9

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Based on 7 publication(s) in Google Scholar

Other Forms of 5'-N-Ethylcarboxamidoadenosine:

5'-N-Ethylcarboxamidoadenosine (Standard) Angebot einholen

7 Publications Citing Use of MCE 5'-N-Ethylcarboxamidoadenosine

•Nanomaterials. 2021 Apr 24;11(5):1102. [Abstract]

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Cell Imaging/Staining

: 5'-N-Ethylcarboxamidoadenosine purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Mar 28;67(6):5075-5092. [Abstract]; WB analysis of inhibition effects of CD73 plasmids, 5'-N-Ethylcarboxamidoadenosine (NECA) (10 μM), or adenosine in RhoA/LIMK/cofilin signaling. 5'-N-Ethylcarboxamidoadenosine (NECA) inhibited the phosphorylation of RhoA-LIMK-cofilin signaling and showed the reduction of p-LIMK and p-cofilin to the maximum extent in AGS cells.

: 5'-N-Ethylcarboxamidoadenosine purchased from MedChemExpress. Usage Cited in: J Med Chem. 2023 Apr 13;66(7):4734-4754. [Abstract]; 5'-N-Ethylcarboxamidoadenosine (NECA) (1 μM) treatment significantly down-regulated the gene expressions of GZMB, IFNG, and IL-2 in Jurkat T cells.

: 5'-N-Ethylcarboxamidoadenosine purchased from MedChemExpress. Usage Cited in: J Med Chem. 2023 Apr 13;66(7):4734-4754. [Abstract]; Cytotoxic T lymphocytes from OT-I mouse splenocyte (OT-I CTL) were co-incubated with MC38-OVA cells for 48 h in the presence of 5'-N-Ethylcarboxamidoadenosine (NECA) (1 μM) with or without compound 38. Then, living MC38-OVA cells were stained with a cell-permeant dye Calcein AM with green fluorescence. 5'-N-Ethylcarboxamidoadenosine (NECA) profoundly impaired the cytotoxic function of OT-ICTL.

: 5'-N-Ethylcarboxamidoadenosine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Mar 23;11(3):202. [Abstract]; OT-I cytotoxic T lymphocytes and MC38-OVA were cocultured with 5'-N-Ethylcarboxamidoadenosine (NECA) (1 μM; 48 h). 5'-N-Ethylcarboxamidoadenosine (NECA), a potent immunosuppressive ligand, notably impaired the effect that OT-I CTLs killed MC38-OVA cancer cells.

: 5'-N-Ethylcarboxamidoadenosine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Mar 23;11(3):202. [Abstract]; Human Jurkat T cells were stimulated with 20 ng/mL PMA and 1 μg/mL ionomycin (IONO) in the presence of 5'-N-Ethylcarboxamidoadenosine (NECA) (1 μM) for 5 h with varied concentrations of compound 7ai (0.1, 1,10 μM). The gene expression of PD-1 and TIM-3 were determined by Q-PCR and standardized using GAPDH as an internal reference.

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Adenosine Receptor Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

Adenosine A₁ receptor (A₁R) Adenosine A_2A receptor (A_2AR) Adenosine A_2B receptor (A_2BR) Adenosine A₃ receptor (A₃R)

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Beschreibung

5'-N-Ethylcarboxamidoadenosine (NECA) is a nonselective adenosine receptor agonist.

IC₅₀ & Target

Adenosine receptor^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	> 10000 1 Compound: 1b, NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method	[PMID: 25780876]
CHO	EC₅₀	>10 3 Compound: 1b, NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method	[PMID: 25780876]
CHO	EC₅₀	0.018 3 Compound: 2 (NECA)	Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as [3H]cAMP accumulation after 15 mins by liquid scintillation counting Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as [3H]cAMP accumulation after 15 mins by liquid scintillation counting	[PMID: 23245803]
CHO	EC₅₀	0.018 3 Compound: 2 (NECA)	Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as [3H]cAMP accumulation after 15 mins by liquid scintillation counting Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as [3H]cAMP accumulation after 15 mins by liquid scintillation counting	[PMID: 23245803]
CHO	EC₅₀	0.04 3 Compound: NECA	G-protein activation in human Adenosine A2A receptor expressing CHO cells using cAMP assay G-protein activation in human Adenosine A2A receptor expressing CHO cells using cAMP assay	[PMID: 11784146]
CHO	EC₅₀	0.04 3 Compound: NECA	G-protein activation in human Adenosine A2A receptor expressing CHO cells using cAMP assay G-protein activation in human Adenosine A2A receptor expressing CHO cells using cAMP assay	[PMID: 11784146]
CHO	EC₅₀	10.2 1 Compound: NECA	Agonist activity at human recombinant adenosine receptor-1 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production preincubated for 45 mins prior forskolin-induction measured after 15 mins by immunoassay Agonist activity at human recombinant adenosine receptor-1 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production preincubated for 45 mins prior forskolin-induction measured after 15 mins by immunoassay	[PMID: 21858244]
CHO	EC₅₀	2.4 3 Compound: NECA	Receptor stimulated adenylyl cyclase activity in CHO cells expressing human recombinant Adenosine A2B receptor Receptor stimulated adenylyl cyclase activity in CHO cells expressing human recombinant Adenosine A2B receptor	[PMID: 11459663]
COS-7	EC₅₀	10.4 3 Compound: NECA	Activity against human adenosine A3 receptor H272E mutant expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay Activity against human adenosine A3 receptor H272E mutant expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay	[PMID: 16640329]
CHO	EC₅₀	12.58 1 Compound: 1b, NECA	Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method	[PMID: 25780876]
CHO	EC₅₀	30 1 Compound: NECA	Agonist activity at human adenosine A1 receptor expressed in CHO cells by GTPPgammaS binding assay Agonist activity at human adenosine A1 receptor expressed in CHO cells by GTPPgammaS binding assay	[PMID: 20031406]
COS-7	EC₅₀	0.078 3 Compound: NECA	Activity against human wild type adenosine A3 receptor expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay Activity against human wild type adenosine A3 receptor expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay	[PMID: 16640329]
CHO	EC₅₀	0.018 3 Compound: 2 (NECA)	Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as [3H]cAMP accumulation after 15 mins by liquid scintillation counting Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as [3H]cAMP accumulation after 15 mins by liquid scintillation counting	[PMID: 23245803]
CHO	EC₅₀	38 1 Compound: NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as effect on cAMP production by luciferase reporter gene assay Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as effect on cAMP production by luciferase reporter gene assay	[PMID: 20031406]
HCT-116	IC₅₀	>10 3 Compound: NECA	Cytotoxicity against human HCT-116 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human HCT-116 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]
CHO	EC₅₀	0.04 3 Compound: NECA	G-protein activation in human Adenosine A2A receptor expressing CHO cells using cAMP assay G-protein activation in human Adenosine A2A receptor expressing CHO cells using cAMP assay	[PMID: 11784146]
CHO	EC₅₀	10.2 1 Compound: NECA	Agonist activity at human recombinant adenosine receptor-1 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production preincubated for 45 mins prior forskolin-induction measured after 15 mins by immunoassay Agonist activity at human recombinant adenosine receptor-1 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production preincubated for 45 mins prior forskolin-induction measured after 15 mins by immunoassay	[PMID: 21858244]
CHO	EC₅₀	10.2 1 Compound: NECA	Agonist activity at human recombinant adenosine receptor-1 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production preincubated for 45 mins prior forskolin-induction measured after 15 mins by immunoassay Agonist activity at human recombinant adenosine receptor-1 expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production preincubated for 45 mins prior forskolin-induction measured after 15 mins by immunoassay	[PMID: 21858244]
CHO	EC₅₀	45 1 Compound: NECA	Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as stimulation of NECA-mediated cAMP accumulation preincubated for 15 mins followed by NECA addition measured after 15 mins by enzyme immunoassay Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as stimulation of NECA-mediated cAMP accumulation preincubated for 15 mins followed by NECA addition measured after 15 mins by enzyme immunoassay	10.1039/C5MD00086F
CHO	EC₅₀	63 1 Compound: NECA	Agonist activity at human adenosine A3 receptor expressed in CHO cells by GTPPgammaS binding assay Agonist activity at human adenosine A3 receptor expressed in CHO cells by GTPPgammaS binding assay	[PMID: 20031406]
HEK293	IC₅₀	3.7 × 10^-8 41 Compound: NECA	Displacement of [3H]CGS 21680 from human recombinant adenosine A2a receptor expressed in HEK293 cells Displacement of [3H]CGS 21680 from human recombinant adenosine A2a receptor expressed in HEK293 cells	[PMID: 26988801]
CHO	EC₅₀	104 1 Compound: NECA	Agonistic activity against human adenosine A2B receptor expressed in CHO cells Agonistic activity against human adenosine A2B receptor expressed in CHO cells	[PMID: 15239649]
CHO-K1	EC₅₀	17.6 1 Compound: 2, NECA	Agonist activity at human adenosine A2A receptor expressed in CHO-K1 cells by cAMP accumulation assay Agonist activity at human adenosine A2A receptor expressed in CHO-K1 cells by cAMP accumulation assay	[PMID: 24900277]
HEK293	IC₅₀	3.7 × 10^-8 41 Compound: NECA	Displacement of [3H]CGS 21680 from human recombinant adenosine receptor A2A expressed in HEK293 cells measured after 120 mins by scintillation counting method Displacement of [3H]CGS 21680 from human recombinant adenosine receptor A2A expressed in HEK293 cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
CHO	EC₅₀	110 1 Compound: 2, NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 17378544]
COS-7	EC₅₀	0.078 3 Compound: NECA	Activity against human wild type adenosine A3 receptor expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay Activity against human wild type adenosine A3 receptor expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay	[PMID: 16640329]
CHO	EC₅₀	12.58 1 Compound: 1b, NECA	Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method	[PMID: 25780876]
CHO	EC₅₀	12.58 1 Compound: 1b, NECA	Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method	[PMID: 25780876]
CHO-K1	EC₅₀	17.6 1 Compound: 2, NECA	Agonist activity at human adenosine A2A receptor expressed in CHO-K1 cells by cAMP accumulation assay Agonist activity at human adenosine A2A receptor expressed in CHO-K1 cells by cAMP accumulation assay	[PMID: 24900277]
COS-7	EC₅₀	10.4 3 Compound: NECA	Activity against human adenosine A3 receptor H272E mutant expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay Activity against human adenosine A3 receptor H272E mutant expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay	[PMID: 16640329]
CHO	EC₅₀	140 1 Compound: 2, NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 17378544]
CHO	EC₅₀	21.9 1 Compound: 2, NECA	Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 17378544]
CHO	EC₅₀	155 1 Compound: NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 17306548]
HCT-116	IC₅₀	> 10 3 Compound: NECA	Cytotoxicity against human HCT-116 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human HCT-116 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]
CHO	EC₅₀	30 1 Compound: NECA	Agonist activity at human adenosine A1 receptor expressed in CHO cells by GTPPgammaS binding assay Agonist activity at human adenosine A1 receptor expressed in CHO cells by GTPPgammaS binding assay	[PMID: 20031406]
CHO	EC₅₀	160 1 Compound: 1, NECA	Activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP levels Activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP levels	[PMID: 17228880]
HEK293	EC₅₀	188 1 Compound: 37; NECA	Partial agonist activity at recombinant mouse A3AR expressed in HEK293 cell membranes assessed as stimulation of [35S]GTPgammaS binding measured after 2 hrs in presence of A2BAR blocker PSB-603 by liquid scintillation counting analysis Partial agonist activity at recombinant mouse A3AR expressed in HEK293 cell membranes assessed as stimulation of [35S]GTPgammaS binding measured after 2 hrs in presence of A2BAR blocker PSB-603 by liquid scintillation counting analysis	[PMID: 32271569]
CHO	EC₅₀	38 1 Compound: NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as effect on cAMP production by luciferase reporter gene assay Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as effect on cAMP production by luciferase reporter gene assay	[PMID: 20031406]
CHO	EC₅₀	2.4 3 Compound: NECA	Receptor stimulated adenylyl cyclase activity in CHO cells expressing human recombinant Adenosine A2B receptor Receptor stimulated adenylyl cyclase activity in CHO cells expressing human recombinant Adenosine A2B receptor	[PMID: 11459663]
HEK293	IC₅₀	3.7 x 10^-2 3 Compound: NECA	Displacement of [3H]CGS 21680 from human recombinant adenosine receptor A2A expressed in HEK293 cells measured after 120 mins by scintillation counting method Displacement of [3H]CGS 21680 from human recombinant adenosine receptor A2A expressed in HEK293 cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
PBMC	IC₅₀	187 1 Compound: NECA	Antiinflammatory activity in rat PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA Antiinflammatory activity in rat PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA	[PMID: 20031406]
PBMC	IC₅₀	40 1 Compound: NECA	Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA	[PMID: 20031406]
CHO	EC₅₀	21.9 1 Compound: 2, NECA	Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 17378544]
CHO	EC₅₀	45 1 Compound: NECA	Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as stimulation of NECA-mediated cAMP accumulation preincubated for 15 mins followed by NECA addition measured after 15 mins by enzyme immunoassay Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as stimulation of NECA-mediated cAMP accumulation preincubated for 15 mins followed by NECA addition measured after 15 mins by enzyme immunoassay	10.1039/C5MD00086F
PBMC	IC₅₀	40 1 Compound: NECA	Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA	[PMID: 20031406]
CHO	EC₅₀	30 1 Compound: NECA	Agonist activity at human adenosine A1 receptor expressed in CHO cells by GTPPgammaS binding assay Agonist activity at human adenosine A1 receptor expressed in CHO cells by GTPPgammaS binding assay	[PMID: 20031406]
CHO	EC₅₀	50 1 Compound: NECA	Potency against cAMP formation in CHO cells expressing recombinant human A2A receptor Potency against cAMP formation in CHO cells expressing recombinant human A2A receptor	[PMID: 15267242]
CHO	EC₅₀	38 1 Compound: NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as effect on cAMP production by luciferase reporter gene assay Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as effect on cAMP production by luciferase reporter gene assay	[PMID: 20031406]
PC-12	EC₅₀	130 1 Compound: 1	Potency against PC12 cell A2 adenosine receptor by adenylate cyclase activation Potency against PC12 cell A2 adenosine receptor by adenylate cyclase activation	[PMID: 3018244]
CHO	EC₅₀	63 1 Compound: NECA	Agonist activity at human adenosine A3 receptor expressed in CHO cells by GTPPgammaS binding assay Agonist activity at human adenosine A3 receptor expressed in CHO cells by GTPPgammaS binding assay	[PMID: 20031406]
PC-3	IC₅₀	> 10 3 Compound: NECA	Cytotoxicity against human PC-3 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human PC-3 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]
CHO	EC₅₀	440 1 Compound: NECA	Potency against cAMP formation in CHO cells expressing recombinant human A2B receptor Potency against cAMP formation in CHO cells expressing recombinant human A2B receptor	[PMID: 15267242]
CHO	EC₅₀	84.8 1 Compound: NECA	Effective concentration for stimulation of [35S]GTP-gamma-S, binding at human adenosine A3 receptor expressed in CHO cells Effective concentration for stimulation of [35S]GTP-gamma-S, binding at human adenosine A3 receptor expressed in CHO cells	[PMID: 15214785]
CHO	EC₅₀	45 1 Compound: NECA	Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as stimulation of NECA-mediated cAMP accumulation preincubated for 15 mins followed by NECA addition measured after 15 mins by enzyme immunoassay Agonist activity at human recombinant adenosine A2A receptor expressed in CHO cells assessed as stimulation of NECA-mediated cAMP accumulation preincubated for 15 mins followed by NECA addition measured after 15 mins by enzyme immunoassay	10.1039/C5MD00086F
WI-38	IC₅₀	> 10 3 Compound: NECA	Cytotoxicity against human WI-38 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human WI-38 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]
CHO	EC₅₀	104 1 Compound: NECA	Agonistic activity against human adenosine A2B receptor expressed in CHO cells Agonistic activity against human adenosine A2B receptor expressed in CHO cells	[PMID: 15239649]
CHO	EC₅₀	50 1 Compound: NECA	Potency against cAMP formation in CHO cells expressing recombinant human A2A receptor Potency against cAMP formation in CHO cells expressing recombinant human A2A receptor	[PMID: 15267242]
CHO	EC₅₀	110 1 Compound: 2, NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 17378544]
CHO	EC₅₀	63 1 Compound: NECA	Agonist activity at human adenosine A3 receptor expressed in CHO cells by GTPPgammaS binding assay Agonist activity at human adenosine A3 receptor expressed in CHO cells by GTPPgammaS binding assay	[PMID: 20031406]
PC-12	EC₅₀	130 1 Compound: 1	Potency against PC12 cell A2 adenosine receptor by adenylate cyclase activation Potency against PC12 cell A2 adenosine receptor by adenylate cyclase activation	[PMID: 3018244]
CHO	EC₅₀	84.8 1 Compound: NECA	Effective concentration for stimulation of [35S]GTP-gamma-S, binding at human adenosine A3 receptor expressed in CHO cells Effective concentration for stimulation of [35S]GTP-gamma-S, binding at human adenosine A3 receptor expressed in CHO cells	[PMID: 15214785]
CHO	EC₅₀	140 1 Compound: 2, NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 17378544]
CHO	EC₅₀	> 10000 1 Compound: 1b, NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of [3H]cAMP levels by scintillation counting method	[PMID: 25780876]
CHO	EC₅₀	155 1 Compound: NECA	Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP production	[PMID: 17306548]
CHO	EC₅₀	160 1 Compound: 1, NECA	Activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP levels Activity at human adenosine A2B receptor expressed in CHO cells assessed as stimulation of cAMP levels	[PMID: 17228880]
PBMC	IC₅₀	187 1 Compound: NECA	Antiinflammatory activity in rat PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA Antiinflammatory activity in rat PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA	[PMID: 20031406]
HEK293	EC₅₀	188 1 Compound: 37; NECA	Partial agonist activity at recombinant mouse A3AR expressed in HEK293 cell membranes assessed as stimulation of [35S]GTPgammaS binding measured after 2 hrs in presence of A2BAR blocker PSB-603 by liquid scintillation counting analysis Partial agonist activity at recombinant mouse A3AR expressed in HEK293 cell membranes assessed as stimulation of [35S]GTPgammaS binding measured after 2 hrs in presence of A2BAR blocker PSB-603 by liquid scintillation counting analysis	[PMID: 32271569]
CHO	EC₅₀	440 1 Compound: NECA	Potency against cAMP formation in CHO cells expressing recombinant human A2B receptor Potency against cAMP formation in CHO cells expressing recombinant human A2B receptor	[PMID: 15267242]
PC-3	IC₅₀	>10 3 Compound: NECA	Cytotoxicity against human PC-3 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human PC-3 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]
WI-38	IC₅₀	>10 3 Compound: NECA	Cytotoxicity against human WI-38 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human WI-38 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]
CHO-K1	EC₅₀	17.6 1 Compound: 2, NECA	Agonist activity at human adenosine A2A receptor expressed in CHO-K1 cells by cAMP accumulation assay Agonist activity at human adenosine A2A receptor expressed in CHO-K1 cells by cAMP accumulation assay	[PMID: 24900277]
COS-7	EC₅₀	0.078 3 Compound: NECA	Activity against human wild type adenosine A3 receptor expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay Activity against human wild type adenosine A3 receptor expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay	[PMID: 16640329]
COS-7	EC₅₀	10.4 3 Compound: NECA	Activity against human adenosine A3 receptor H272E mutant expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay Activity against human adenosine A3 receptor H272E mutant expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay	[PMID: 16640329]
HCT-116	IC₅₀	> 10 3 Compound: NECA	Cytotoxicity against human HCT-116 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human HCT-116 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]
HEK293	EC₅₀	188 1 Compound: 37; NECA	Partial agonist activity at recombinant mouse A3AR expressed in HEK293 cell membranes assessed as stimulation of [35S]GTPgammaS binding measured after 2 hrs in presence of A2BAR blocker PSB-603 by liquid scintillation counting analysis Partial agonist activity at recombinant mouse A3AR expressed in HEK293 cell membranes assessed as stimulation of [35S]GTPgammaS binding measured after 2 hrs in presence of A2BAR blocker PSB-603 by liquid scintillation counting analysis	[PMID: 32271569]
HEK293	IC₅₀	3.7 x 10^-8 41 Compound: NECA	Displacement of [3H]CGS 21680 from human recombinant adenosine A2a receptor expressed in HEK293 cells Displacement of [3H]CGS 21680 from human recombinant adenosine A2a receptor expressed in HEK293 cells	[PMID: 26988801]
HEK293	IC₅₀	3.7 x 10^-8 41 Compound: NECA	Displacement of [3H]CGS 21680 from human recombinant adenosine receptor A2A expressed in HEK293 cells measured after 120 mins by scintillation counting method Displacement of [3H]CGS 21680 from human recombinant adenosine receptor A2A expressed in HEK293 cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
PBMC	IC₅₀	187 1 Compound: NECA	Antiinflammatory activity in rat PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA Antiinflammatory activity in rat PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA	[PMID: 20031406]
PBMC	IC₅₀	40 1 Compound: NECA	Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 20 hrs by ELISA	[PMID: 20031406]
PC-12	EC₅₀	130 1 Compound: 1	Potency against PC12 cell A2 adenosine receptor by adenylate cyclase activation Potency against PC12 cell A2 adenosine receptor by adenylate cyclase activation	[PMID: 3018244]
PC-3	IC₅₀	> 10 3 Compound: NECA	Cytotoxicity against human PC-3 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human PC-3 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]
WI-38	IC₅₀	> 10 3 Compound: NECA	Cytotoxicity against human WI-38 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay Cytotoxicity against human WI-38 cells assessed as cell viability incubated for 72 to 96 hrs by MTT assay	[PMID: 35707146]

In Vivo

After the administration of 5'-N-Ethylcarboxamidoadenosine (NECA), the mean number of cocaine infusions obtained per session is decreased significantly in a dose-dependent manner [5'-N-Ethylcarboxamidoadenosine (NECA): F(4,12)=14.9; P<0.001]. The administration of 5'-N-Ethylcarboxamidoadenosine (NECA) [F(4,12)=16.1; P<0.001] results in a significant increase in latencies above values obtained for vehicle treatment^[1]. Daily i.p. injection of 5'-N-Ethylcarboxamidoadenosine (NECA) at 0.3 mg/kg/day for two weeks reduces malondialdehyde (MDA) levels in diabetic rats, but does not affect control rats. Daily treatment with NECA (0.3 mg/kg/day, i.p. for two weeks) reduces diabetes-induced gene expression of tumor necrosis factor (TNF)-α and interleukin (IL)-18 in diabetic rats, but does not affect control rats. Daily i.p. injection of 5'-N-Ethylcarboxamidoadenosine (NECA) at 0.3 mg/kg/day for two weeks also blocks the activation of JNK MAPK in diabetic rats, but does not affect control rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

308.29

Formel

C₁₂H₁₆N₆O₄

CAS. Nr.

35920-39-9

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@H](N2C=NC3=C(N)N=CN=C23)O[C@H](C(NCC)=O)[C@H]1O

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 62.5 mg/mL (202.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2437 mL	16.2185 mL	32.4370 mL
5 mM	0.6487 mL	3.2437 mL	6.4874 mL
10 mM	0.3244 mL	1.6218 mL	3.2437 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Reinheit & Dokumentation

Purity: 99.09%

Select Batch:

Data Sheet (277 KB) SDS (479 KB)

COA (251 KB) HNMR (260 KB) LCMS (112 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Knapp CM, et al. Adenosine agonists CGS 21680 and NECA inhibit the initiation of cocaine self-administration. Pharmacol Biochem Behav. 2001 Apr;68(4):797-803. [Content Brief]

[2]. Elsherbiny NM, et al. Reno-protective effect of NECA in diabetic nephropathy: implication of IL-18 and ICAM-1. Eur Cytokine Netw. 2012 Jul-Sep;23(3):78-86. [Content Brief]

Tierverwaltung
^[1]

Male Wistar rats are used in this experiment. These animals range in weight between 350 and 425 g. They are housed individually in hanging wire cages under a 12-h light/dark cycle. The effects of 5'-N-Ethylcarboxamidoadenosine (NECA) are tested in four rats. In the experiment, either 5'-N-Ethylcarboxamidoadenosine (NECA) (5, 7.5, 10, 20 μg/kg) or vehicle (saline) is administered intraperitoneally 15 min prior to the start of test sessions. 5'-N-Ethylcarboxamidoadenosine (NECA) is administered following a random order crossover design. In most cases, animals are tested twice with the same dose^[1].

MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.

Verweise

[1]. Knapp CM, et al. Adenosine agonists CGS 21680 and NECA inhibit the initiation of cocaine self-administration. Pharmacol Biochem Behav. 2001 Apr;68(4):797-803. [Content Brief]

[2]. Elsherbiny NM, et al. Reno-protective effect of NECA in diabetic nephropathy: implication of IL-18 and ICAM-1. Eur Cytokine Netw. 2012 Jul-Sep;23(3):78-86. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2437 mL	16.2185 mL	32.4370 mL	81.0925 mL
	5 mM	0.6487 mL	3.2437 mL	6.4874 mL	16.2185 mL
	10 mM	0.3244 mL	1.6218 mL	3.2437 mL	8.1092 mL
	15 mM	0.2162 mL	1.0812 mL	2.1625 mL	5.4062 mL
	20 mM	0.1622 mL	0.8109 mL	1.6218 mL	4.0546 mL
	25 mM	0.1297 mL	0.6487 mL	1.2975 mL	3.2437 mL
	30 mM	0.1081 mL	0.5406 mL	1.0812 mL	2.7031 mL
	40 mM	0.0811 mL	0.4055 mL	0.8109 mL	2.0273 mL
	50 mM	0.0649 mL	0.3244 mL	0.6487 mL	1.6218 mL
	60 mM	0.0541 mL	0.2703 mL	0.5406 mL	1.3515 mL
	80 mM	0.0405 mL	0.2027 mL	0.4055 mL	1.0137 mL
	100 mM	0.0324 mL	0.1622 mL	0.3244 mL	0.8109 mL

5'-N-Ethylcarboxamidoadenosine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5'-N-Ethylcarboxamidoadenosine35920-39-9NECAAdenosine ReceptorP1 receptorInhibitorinhibitorinhibit

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5'-N-Ethylcarboxamidoadenosine (Synonyms: NECA)

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Other Forms of 5'-N-Ethylcarboxamidoadenosine:

5'-N-Ethylcarboxamidoadenosine Related Antibodies

7 Publications Citing Use of MCE 5'-N-Ethylcarboxamidoadenosine

Spezialitätsempfehlungen

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Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

5'-N-Ethylcarboxamidoadenosine Related Classifications

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