2. GPCR/G Protein
  3. Angiotensin Receptor
  4. Sodium nitroprusside

Sodium nitroprusside  (Synonyms: Ro 21-2498)

Cat. No.: HY-B0564 Assay: 99.99%
COA
SDS
Instrucciones de manejo Technical Support

Sodium nitroprusside (Ro 21-2498) is a potent vasodilator working through releasing NO spontaneously in blood.

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No. CAS : 14402-89-2

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
100 mg En stock
500 mg En stock
1 g   Obtener un presupuesto  
5 g   Obtener un presupuesto  

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Revisión del cliente

Based on 17 publication(s) in Google Scholar

Other Forms of Sodium nitroprusside:

Sodium nitroprusside Related Antibodies

Top Publications Citing Use of Products

    Sodium nitroprusside purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103004.  [Abstract]

    Sodium nitroprusside (SNP, 10⁻⁹ to 10⁻⁵ mol/L) mediated endothelium-independent vasodilatation was only mildly influenced in ACE2-KI mice following CLP surgery.

    Sodium nitroprusside purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103004.  [Abstract]

    Sodium nitroprusside (SNP, 10⁻⁹ to 10⁻⁵ mol/L)-mediated endothelium-independent vasodilatation was not influenced in ACE2-KO mice after CLP surgery.

    Sodium nitroprusside purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49.  [Abstract]

    Representative images of immunofluorescence analysis of Nrf2 expression in microglia treated with the AKT inhibitor MK2206 (5 μM，6 h), the GSK3β activator sodium nitroprusside (SNP, 100 μM， 6h), and the GSK3β inhibitor TDZD-8 (5 μM，6 h). Nrf2 (red), nuclei (blue).

    Sodium nitroprusside purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49.  [Abstract]

    Representative western blots and quantitative data for the expression of Nrf2 in the nucleus and the downstream proteins of Nrf2 pathway, including HO-1 and NQO1, with different treatments (MK2206: 5 μM，6 h; SNP: 100 μM， 6h; TDZD-8: 5 μM，6 h).

    Sodium nitroprusside purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49.  [Abstract]

    Nrf2 knockout abolished neurological function improvement mediated by combined curcumin-hUC-MSC treatment. TTC-stained brain sections (A) and quantitative analysis (B) showed the decreased infarct volume with combined therapy in MCAO mice (MK2206, SNP or TDZD-8 :5 mg/kg， intraperitoneally，3 consecutive days after MCAO).

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    • Actividad biológica

    • Pureza y Documentación

    • Referencias

    • Revisión del cliente

    Descripciòn

    Sodium nitroprusside (Ro 21-2498) is a potent vasodilator working through releasing NO spontaneously in blood.

    In Vitro

    Sodium nitroprusside (Ro 21-2498) is a potent vasodilator. Sodium nitroprusside has potent vasodilating effects in arterioles and venules. Sodium nitroprusside breaks down in circulation to release nitric oxide (NO). NO activates guanylate cyclase in vascular smooth muscle and increases intracellular production of cGMP. The end result is vascular smooth muscle relaxation, which allow vessels to dilate[1].
    Sodium nitroprusside decreases the proliferation of vascular smooth muscle cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sodium nitroprusside Related Antibodies
    In Vivo

    Sodium nitroprusside (5 mg/kg) significantly reduces the intestinal ischemiareperfusion injury as a nitric oxide donor in rats[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Peso molecular

    261.92

    Fòrmula

    C5FeN6Na2O
    No. CAS
    Appearance

    Solid

    Color

    Brown to reddish brown

    SMILES

    N#C[Fe-2](C#N)(C#N)(C#N)(N=O)C#N.[Na+].[Na+]
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvente y solubilidad
    In Vitro: 

    H2O : 100 mg/mL (381.80 mM; Need ultrasonic)

    DMSO : 33.33 mg/mL (127.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.8180 mL 19.0898 mL 38.1796 mL
    5 mM 0.7636 mL 3.8180 mL 7.6359 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.54 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (9.54 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (381.80 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureza y Documentación

    Purity: 99.99%

    SDS (479 KB)

    COA (255 KB)

    Instrucciones de manejo (2659 KB)
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.8180 mL 19.0898 mL 38.1796 mL 95.4490 mL
    5 mM 0.7636 mL 3.8180 mL 7.6359 mL 19.0898 mL
    10 mM 0.3818 mL 1.9090 mL 3.8180 mL 9.5449 mL
    15 mM 0.2545 mL 1.2727 mL 2.5453 mL 6.3633 mL
    20 mM 0.1909 mL 0.9545 mL 1.9090 mL 4.7724 mL
    25 mM 0.1527 mL 0.7636 mL 1.5272 mL 3.8180 mL
    30 mM 0.1273 mL 0.6363 mL 1.2727 mL 3.1816 mL
    40 mM 0.0954 mL 0.4772 mL 0.9545 mL 2.3862 mL
    50 mM 0.0764 mL 0.3818 mL 0.7636 mL 1.9090 mL
    60 mM 0.0636 mL 0.3182 mL 0.6363 mL 1.5908 mL
    80 mM 0.0477 mL 0.2386 mL 0.4772 mL 1.1931 mL
    100 mM 0.0382 mL 0.1909 mL 0.3818 mL 0.9545 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Sodium nitroprusside Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sodium nitroprusside14402-89-2Ro 21-2498Angiotensin ReceptorInhibitorinhibitorinhibit

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    Nombre del producto:
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