2. Autophagy
  3. Autophagy
  4. Nitroprusside disodium dihydrate

Nitroprusside disodium dihydrate  (Synonyms: Sodium nitroprusside dihydrate; Sodium Nitroferricyanide(III) Dihydrate)

Cat. No.: HY-A0119 Purity: 99.62%
COA
SDS
Handling Instructions Technical Support

Nitroprusside disodium dehydrate (Sodium nitroprusside dihydrate) is a vasodilator that available for the research of acute hypertension, heart failure. Nitroprusside disodium dehydrate induces autophagy in glutathione-depleted osteoblasts. Nitroprusside disodium dehydrate acts as a nitric oxide (NO) donor in a rat intestinal ischemia reperfusion model.

For research use only. We do not sell to patients.

CAS No. : 13755-38-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
10 g In-stock
50 g   Get quote  

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Customer Review

Based on 17 publication(s) in Google Scholar

Other Forms of Nitroprusside disodium dihydrate:

Nitroprusside disodium dihydrate Related Antibodies

Top Publications Citing Use of Products

    Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103004.  [Abstract]

    Sodium nitroprusside (SNP, 10⁻⁹ to 10⁻⁵ mol/L) mediated endothelium-independent vasodilatation was only mildly influenced in ACE2-KI mice following CLP surgery.

    Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103004.  [Abstract]

    Sodium nitroprusside (SNP, 10⁻⁹ to 10⁻⁵ mol/L)-mediated endothelium-independent vasodilatation was not influenced in ACE2-KO mice after CLP surgery.

    Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49.  [Abstract]

    Representative images of immunofluorescence analysis of Nrf2 expression in microglia treated with the AKT inhibitor MK2206 (5 μM，6 h), the GSK3β activator sodium nitroprusside (SNP, 100 μM， 6h), and the GSK3β inhibitor TDZD-8 (5 μM，6 h). Nrf2 (red), nuclei (blue).

    Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49.  [Abstract]

    Representative western blots and quantitative data for the expression of Nrf2 in the nucleus and the downstream proteins of Nrf2 pathway, including HO-1 and NQO1, with different treatments (MK2206: 5 μM，6 h; SNP: 100 μM， 6h; TDZD-8: 5 μM，6 h).

    Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49.  [Abstract]

    Nrf2 knockout abolished neurological function improvement mediated by combined curcumin-hUC-MSC treatment. TTC-stained brain sections (A) and quantitative analysis (B) showed the decreased infarct volume with combined therapy in MCAO mice (MK2206, SNP or TDZD-8 :5 mg/kg， intraperitoneally，3 consecutive days after MCAO).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Nitroprusside disodium dehydrate (Sodium nitroprusside dihydrate) is a vasodilator that available for the research of acute hypertension, heart failure. Nitroprusside disodium dehydrate induces autophagy in glutathione-depleted osteoblasts. Nitroprusside disodium dehydrate acts as a nitric oxide (NO) donor in a rat intestinal ischemia reperfusion model[1][2][3][4].

    In Vitro

    Nitroprusside disodium dehydrate sensitizes human gastric cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nitroprusside disodium dihydrate Related Antibodies
    Clinical Trial
    Molecular Weight

    297.95

    Formula

    C5H4FeN6Na2O3
    CAS No.
    Appearance

    Solid

    Color

    Reddish brown to dark brown

    SMILES

    [Na2[Fe(CN)5NO].2H2O]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (335.63 mM; Need ultrasonic)

    DMSO : 50 mg/mL (167.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3563 mL 16.7813 mL 33.5627 mL
    5 mM 0.6713 mL 3.3563 mL 6.7125 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Concentration (final)

    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (335.63 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.62%

    SDS (480 KB)

    COA (243 KB) RP-HPLC (240 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.3563 mL 16.7813 mL 33.5627 mL 83.9067 mL
    5 mM 0.6713 mL 3.3563 mL 6.7125 mL 16.7813 mL
    10 mM 0.3356 mL 1.6781 mL 3.3563 mL 8.3907 mL
    15 mM 0.2238 mL 1.1188 mL 2.2375 mL 5.5938 mL
    20 mM 0.1678 mL 0.8391 mL 1.6781 mL 4.1953 mL
    25 mM 0.1343 mL 0.6713 mL 1.3425 mL 3.3563 mL
    30 mM 0.1119 mL 0.5594 mL 1.1188 mL 2.7969 mL
    40 mM 0.0839 mL 0.4195 mL 0.8391 mL 2.0977 mL
    50 mM 0.0671 mL 0.3356 mL 0.6713 mL 1.6781 mL
    60 mM 0.0559 mL 0.2797 mL 0.5594 mL 1.3984 mL
    80 mM 0.0420 mL 0.2098 mL 0.4195 mL 1.0488 mL
    100 mM 0.0336 mL 0.1678 mL 0.3356 mL 0.8391 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Nitroprusside disodium dihydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nitroprusside disodium dihydrate13755-38-9Sodium nitroprusside dihydrate Sodium Nitroferricyanide(III) DihydrateAutophagylowerbloodpressureheartfailuresurgerydecreasebleeding aorticvalvestenosisInhibitorinhibitorinhibit

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