Nitroprusside disodium dihydrate
Based on 19 publication(s) in Google Scholar
Nitroprusside disodium dehydrate (Sodium nitroprusside dihydrate) is a vasodilator that available for the research of acute hypertension, heart failure. Nitroprusside disodium dehydrate induces autophagy in glutathione-depleted osteoblasts. Nitroprusside disodium dehydrate acts as a nitric oxide (NO) donor in a rat intestinal ischemia reperfusion model.
For research use only. We do not sell to patients.
- Purity: 99.62%
- CAS No.: 13755-38-9
- Formula: C5H4FeN6Na2O3
- Molecular Weight:297.95
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Nitroprusside disodium dihydrate
More- Redox Biol. 2024 Feb:69:103004. [Abstract]
- MedComm (2020). 2026 Mar 28;7(4):e70706. [Abstract]
- Chem Eng J. 15 August 2022, 136169.
- J Neuroinflammation. 2023 Feb 24;20(1):49. [Abstract]
- Br J Pharmacol. 2021 Dec;178(23):4646-4662. [Abstract]
- J Invest Dermatol. 2025 Sep 15:S0022-202X(25)02428-5. [Abstract]
- J Ethnopharmacol. 2025 Jan 10:336:118727. [Abstract]
- Biochem Pharmacol. 2026 Jun 18;251(Pt 2):118179. [Abstract]
- Plant J. 2025 Jul;123(2):e70379. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2024 Oct;1870(7):167437. [Abstract]
- Mol Cell Biochem. 2025 Aug 3. [Abstract]
- FASEB J. 2025 Dec 31;39(24):e71345. [Abstract]
- Appl Biochem Biotechnol. 2025 Mar 25. [Abstract]
- Neuroscience. 2024 Dec 17:563:175-187. [Abstract]
- Exp Eye Res. 2024 Jun:243:109886. [Abstract]
- Theriogenology. 2025 Oct 1:245:117517. [Abstract]
- Phytochem Lett. 2025 Oct 17;70:104058.
- Turk J Med Sci. 2025 Feb 19;55(2):509-517. [Abstract]
- bioRxiv. 2026 Mar 28.
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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IF
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WB
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In Vivo Imaging
Biological Activity
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 13755-38-9
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Appearance Solid
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Molecular Weight 297.95
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Formula C5H4FeN6Na2O3
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Color Reddish brown to dark brown
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SMILES
[Na2[Fe(CN)5NO].2H2O]
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Synonyms
Sodium nitroprusside dihydrate; Sodium Nitroferricyanide(III) Dihydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (19)
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Journal Impact Factor
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Most Recent
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Redox Biol
Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1-7)-Mas-mediated macrophage polarization. [Abstract]2024 Feb:69:103004. PMID: 38141575
Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103004. [Abstract]
Sodium nitroprusside (SNP, 10⁻⁹ to 10⁻⁵ mol/L) mediated endothelium-independent vasodilatation was only mildly influenced in ACE2-KI mice following CLP surgery.
Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103004. [Abstract]
Sodium nitroprusside (SNP, 10⁻⁹ to 10⁻⁵ mol/L)-mediated endothelium-independent vasodilatation was not influenced in ACE2-KO mice after CLP surgery.
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MedComm (2020)
2026 Mar 28;7(4):e70706. PMID: 41930327 -
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J Neuroinflammation
Human umbilical cord-derived mesenchymal stem cell transplantation supplemented with curcumin improves the outcomes of ischemic stroke via AKT/GSK-3β/β-TrCP/Nrf2 axis. [Abstract]2023 Feb 24;20(1):49. PMID: 36829224
Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49. [Abstract]
Representative images of immunofluorescence analysis of Nrf2 expression in microglia treated with the AKT inhibitor MK2206 (5 μM，6 h), the GSK3β activator sodium nitroprusside (SNP, 100 μM， 6h), and the GSK3β inhibitor TDZD-8 (5 μM，6 h). Nrf2 (red), nuclei (blue).
Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49. [Abstract]
Representative western blots and quantitative data for the expression of Nrf2 in the nucleus and the downstream proteins of Nrf2 pathway, including HO-1 and NQO1, with different treatments (MK2206: 5 μM，6 h; SNP: 100 μM， 6h; TDZD-8: 5 μM，6 h).
Nitroprusside disodium dihydrate purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49. [Abstract]
Nrf2 knockout abolished neurological function improvement mediated by combined curcumin-hUC-MSC treatment. TTC-stained brain sections (A) and quantitative analysis (B) showed the decreased infarct volume with combined therapy in MCAO mice (MK2206, SNP or TDZD-8 :5 mg/kg， intraperitoneally，3 consecutive days after MCAO).
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Br J Pharmacol
Transient Receptor Potential channels, TRPV1 and TRPA1 in melanocytes synergize UV-dependent and UV-independent melanogenesis. [Abstract]2021 Dec;178(23):4646-4662. PMID: 34363226 -
J Invest Dermatol
Inducible Nitric Oxide Deficiency Attenuates Necroptosis in Psoriasis-like Dermatitis in Mice through Reducing Oxidative Stress. [Abstract]2025 Sep 15:S0022-202X(25)02428-5. PMID: 40962201 -
J Ethnopharmacol
Evidence to support cultivated fruiting body of Ophiocordyceps sinensis (Ascomycota)'s role in relaxing airway smooth muscle. [Abstract]2025 Jan 10:336:118727. PMID: 39182700 -
Biochem Pharmacol
Dysfunction in the SOCE/EDH-mediated vasorelaxation of mesenteric arteries impairs intestinal barrier integrity in irritable bowel syndrome. [Abstract]2026 Jun 18;251(Pt 2):118179. PMID: 42315077 -
Plant J
Nitric oxide delays floral transition in Arabidopsis by inhibiting histone deacetylases HDA5 and HDA6. [Abstract]2025 Jul;123(2):e70379. PMID: 40726229 -
Biochim Biophys Acta Mol Basis Dis
Methylglyoxal induces endothelial cell apoptosis and coronary microvascular dysfunction through regulating AR-cPLA2 signaling. [Abstract]2024 Oct;1870(7):167437. PMID: 39067539 -
Mol Cell Biochem
Nitric oxide induces apoptosis of human primary melanocytes by regulating calcium homeostasis via VDAC1. [Abstract]2025 Aug 3. PMID: 40753527 -
FASEB J
Natriuretic Peptide Improves Impaired Baroreceptor Sensitivity in a Sex-Dependent Manner by Enhancing Baroreflex Afferent Neuroexcitation Under Physiological and Hypertensive Conditions. [Abstract]2025 Dec 31;39(24):e71345. PMID: 41395769 -
Appl Biochem Biotechnol
Astragaloside IV Relieves Mitochondrial Oxidative Stress Damage and Dysfunction in Diabetic Mice Endothelial Progenitor Cells by Regulating the GSK-3β/Nrf2 Axis. [Abstract]2025 Mar 25. PMID: 40131628 -
Neuroscience
METTL3 inhibits microglial pyroptosis in neonatal hypoxia-ischemia encephalopathy by regulating GPR39 expression in an m6A-HuR-dependent manner. [Abstract]2024 Dec 17:563:175-187. PMID: 39461660 -
Exp Eye Res
2024 Jun:243:109886. PMID: 38583755 -
Theriogenology
2025 Oct 1:245:117517. PMID: 40460471 -
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Turk J Med Sci
2025 Feb 19;55(2):509-517. PMID: 40342326 -
Solvent & Solubility
H2O : 100 mg/mL (335.63 mM; Need ultrasonic)
DMSO : 50 mg/mL (167.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (335.63 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. J N Cohn, et al. Nitroprusside. Ann Intern Med. 1979 Nov;91(5):752-7. [Content Brief]
[2]. Namazi, H., Sodium nitroprusside as a nitric oxide donor in a rat intestinal ischemia reperfusion model: a novel molecular mechanism. Clinics (Sao Paulo), 2008. 63(3): p. 405. [Content Brief]
[3]. Min Jeong Son, et al. Sodium nitroprusside induces autophagic cell death in glutathione-depleted osteoblasts. J Biochem Mol Toxicol. Sep-Oct 2010;24(5):313-22. [Content Brief]
[4]. Liuqin Yang, et al. Sodium nitroprusside (SNP) sensitizes human gastric cancer cells to TRAIL-induced apoptosis. Int Immunopharmacol. 2013 Oct;17(2):383-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.3563 mL | 16.7813 mL | 33.5627 mL | 83.9067 mL |
| 5 mM | 0.6713 mL | 3.3563 mL | 6.7125 mL | 16.7813 mL | |
| 10 mM | 0.3356 mL | 1.6781 mL | 3.3563 mL | 8.3907 mL | |
| 15 mM | 0.2238 mL | 1.1188 mL | 2.2375 mL | 5.5938 mL | |
| 20 mM | 0.1678 mL | 0.8391 mL | 1.6781 mL | 4.1953 mL | |
| 25 mM | 0.1343 mL | 0.6713 mL | 1.3425 mL | 3.3563 mL | |
| 30 mM | 0.1119 mL | 0.5594 mL | 1.1188 mL | 2.7969 mL | |
| 40 mM | 0.0839 mL | 0.4195 mL | 0.8391 mL | 2.0977 mL | |
| 50 mM | 0.0671 mL | 0.3356 mL | 0.6713 mL | 1.6781 mL | |
| 60 mM | 0.0559 mL | 0.2797 mL | 0.5594 mL | 1.3984 mL | |
| 80 mM | 0.0420 mL | 0.2098 mL | 0.4195 mL | 1.0488 mL | |
| 100 mM | 0.0336 mL | 0.1678 mL | 0.3356 mL | 0.8391 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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