Protirelin acetate (Synonyms: Thyrotropin-releasing-hormone acetate; TRH acetate)

Name: Protirelin acetate
Brand: MedChemExpress
SKU: HY-P0002A
Rating: 5.0 (3 reviews)

Cat. No.: HY-P0002A Purity: 99.93%: Data Sheet
SDS

COA
Handling Instructions
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Protirelin Acetate is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Protirelin acetate Chemical Structure

CAS No. : 120876-23-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 3 publication(s) in Google Scholar

Other Forms of Protirelin acetate:

Thyrotropin-Releasing Hormone (TRH), Free Acid Get quote
Protirelin Get quote

3 Publications Citing Use of MCE Protirelin acetate

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Protirelin Acetate is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

IC₅₀ & Target

Thyroid hormone receptor

In Vivo

Protirelin (TRH) is an evolutionarily ancient neuropeptide, having its origin before the divergence of protostomes and deuterostomes, and may ancestrally have been involved in the control of postembryonic growth and reproduction^[1]. The effect of the thyrotropin-releasing hormone (Protirelin, TRH), one of the hypothalamic releasing hormones, on body temperature is investigated in the rat. Protirelin, in doses of 1, 5, 10 and 20 mg/kg, is injected intraperitoneally to male Wistar rats weighing 200-250 g. Protirelin causes a temporary rise in body temperature dose-dependently. The thyroidectomized rats injected 20 mg/kg of Protirelin which induces a significant hyperthermia in the sham-operated animals, fail to show a rise in body temperature. The present results suggest that a release of thyroid hormone might participate in the hyperthermic action of Protirelin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

452.46

Formula

C₁₆H₂₂N₆O₄.3/2C₂H₄O₂

CAS No.

120876-23-5

Appearance

Solid

Color

White to off-white

Sequence

{pGlu}-His-Pro-NH2

Sequence Shortening

{pGlu}-HP-NH2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 233.33 mg/mL (515.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (110.51 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2101 mL	11.0507 mL	22.1014 mL
5 mM	0.4420 mL	2.2101 mL	4.4203 mL
10 mM	0.2210 mL	1.1051 mL	2.2101 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (221.01 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (282 KB) SDS (252 KB)

COA (258 KB) HNMR (258 KB) RP-HPLC (223 KB)

Handling Instructions (2659 KB)

References

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief]

[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Animal Administration
^[2]

Rats^[2]
Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief]

[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.2101 mL	11.0507 mL	22.1014 mL	55.2535 mL
	5 mM	0.4420 mL	2.2101 mL	4.4203 mL	11.0507 mL
	10 mM	0.2210 mL	1.1051 mL	2.2101 mL	5.5254 mL
	15 mM	0.1473 mL	0.7367 mL	1.4734 mL	3.6836 mL
	20 mM	0.1105 mL	0.5525 mL	1.1051 mL	2.7627 mL
	25 mM	0.0884 mL	0.4420 mL	0.8841 mL	2.2101 mL
	30 mM	0.0737 mL	0.3684 mL	0.7367 mL	1.8418 mL
	40 mM	0.0553 mL	0.2763 mL	0.5525 mL	1.3813 mL
	50 mM	0.0442 mL	0.2210 mL	0.4420 mL	1.1051 mL
	60 mM	0.0368 mL	0.1842 mL	0.3684 mL	0.9209 mL
	80 mM	0.0276 mL	0.1381 mL	0.2763 mL	0.6907 mL
	100 mM	0.0221 mL	0.1105 mL	0.2210 mL	0.5525 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Protirelin acetate Related Classifications

Inflammation/Immunology Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Protirelin120876-23-5Thyrotropin-releasing-hormoneTRHThyroid Hormone ReceptorTHRInhibitorinhibitorinhibit

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Protirelin acetate (Synonyms: Thyrotropin-releasing-hormone acetate; TRH acetate)

Customer Review

Other Forms of Protirelin acetate:

Protirelin acetate Related Antibodies

3 Publications Citing Use of MCE Protirelin acetate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Protirelin acetate Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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