2. Vitamin D Related/Nuclear Receptor
  3. Thyroid Hormone Receptor
  4. Protirelin acetate

Protirelin acetate  (Synonyms: Thyrotropin-releasing-hormone acetate; TRH acetate)

Cat. No.: HY-P0002A Purity: 99.84%
COA Handling Instructions

Protirelin Acetate is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Protirelin acetate Chemical Structure

Protirelin acetate Chemical Structure

CAS No. : 120876-23-5

Size Price Stock Quantity
Solution
10 mM * 1 mL in Water USD 57 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 57 In-stock
Solid
5 mg USD 52 In-stock
10 mg USD 84 In-stock
50 mg USD 120 In-stock
100 mg USD 192 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of Protirelin acetate:

Protirelin acetate Related Antibodies

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Description

Protirelin Acetate is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

IC50 & Target

Thyroid hormone receptor
In Vivo

Protirelin (TRH) is an evolutionarily ancient neuropeptide, having its origin before the divergence of protostomes and deuterostomes, and may ancestrally have been involved in the control of postembryonic growth and reproduction[1]. The effect of the thyrotropin-releasing hormone (Protirelin, TRH), one of the hypothalamic releasing hormones, on body temperature is investigated in the rat. Protirelin, in doses of 1, 5, 10 and 20 mg/kg, is injected intraperitoneally to male Wistar rats weighing 200-250 g. Protirelin causes a temporary rise in body temperature dose-dependently. The thyroidectomized rats injected 20 mg/kg of Protirelin which induces a significant hyperthermia in the sham-operated animals, fail to show a rise in body temperature. The present results suggest that a release of thyroid hormone might participate in the hyperthermic action of Protirelin[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

452.46

Appearance

Solid

Formula

C16H22N6O4.3/2C2H4O2
CAS No.
Sequence

{pGlu}-His-Pro-NH2
Sequence Shortening

{pGlu}-HP-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (552.54 mM; Need ultrasonic)

H2O : 50 mg/mL (110.51 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2101 mL 11.0507 mL 22.1014 mL
5 mM 0.4420 mL 2.2101 mL 4.4203 mL
10 mM 0.2210 mL 1.1051 mL 2.2101 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (221.01 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.60 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.98%

COA (258 KB) HNMR (258 KB) RP-HPLC (223 KB)

Handling Instructions (2659 KB)
References
Animal Administration
[2]

Rats[2]
Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Protirelin acetate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Protirelin120876-23-5Thyrotropin-releasing-hormoneTRHThyroid Hormone ReceptorTHRInhibitorinhibitorinhibit

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