2. Peptides
  3. Thyrotropin-Releasing Hormone (TRH), Free Acid

Thyrotropin-Releasing Hormone (TRH), Free Acid  (Synonyms: TRH-OH)

Cat. No.: HY-P1529 Purity: 99.73%
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Thyrotropin-Releasing Hormone (TRH), Free Acid (TRH-OH) is a physiological metabolite of Thyrotropin-Releasing Hormone.

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Thyrotropin-Releasing Hormone (TRH), Free Acid Chemical Structure

Thyrotropin-Releasing Hormone (TRH), Free Acid Chemical Structure

CAS No. : 24769-58-2

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5 mg USD 56 In-stock
10 mg USD 90 In-stock
25 mg USD 140 In-stock
50 mg USD 190 In-stock
100 mg USD 290 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Thyrotropin-Releasing Hormone (TRH), Free Acid:

Thyrotropin-Releasing Hormone (TRH), Free Acid Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Thyrotropin-Releasing Hormone (TRH), Free Acid

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Thyrotropin-Releasing Hormone (TRH), Free Acid (TRH-OH) is a physiological metabolite of Thyrotropin-Releasing Hormone.

In Vitro

TRH degradation products have been shown to be associated with a number of endocrine- and central nervous system-related biological functions. TRH (pGlu-His-Pro-NH2) can be enzymatically degraded in plasma and brain tissue to yield TRH-OH. TRH and hGHRH stimulate the release of [3H]GH into the culture medium to 435 and 464%, respectively, when compared to the control, but TRH-OH has no effect[1]. TRH-OH, produced after cleavage of the C-terminal amide group by a specific proline endopeptidase, is one of the most stable derivatives of TRH and is found at high levels in numerous brain regions. TRH-OH inhibits Na+ channel activity in mammalian septal neurons. In about 60% of the cells tested, TRH-OH at concentrations between 0.01 and 2.5 μM produces a dose-dependent reversible attenuation of Na+ currents. With 2 μM TRH-OH, peak Na+ current amplitude is reduced by 20-50%[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Thyrotropin-Releasing Hormone (TRH), Free Acid Related Antibodies
Molecular Weight

363.37

Formula

C16H21N5O5
CAS No.
Appearance

Solid

Color

Off-white to light yellow

Sequence

{pGlu}-His-Pro
Sequence Shortening

{pGlu}-HP
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (275.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7520 mL 13.7601 mL 27.5202 mL
5 mM 0.5504 mL 2.7520 mL 5.5040 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (275.20 mM); Clear solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  Saline

    Solubility: 10 mg/mL (27.52 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.7520 mL 13.7601 mL 27.5202 mL 68.8004 mL
5 mM 0.5504 mL 2.7520 mL 5.5040 mL 13.7601 mL
10 mM 0.2752 mL 1.3760 mL 2.7520 mL 6.8800 mL
15 mM 0.1835 mL 0.9173 mL 1.8347 mL 4.5867 mL
20 mM 0.1376 mL 0.6880 mL 1.3760 mL 3.4400 mL
25 mM 0.1101 mL 0.5504 mL 1.1008 mL 2.7520 mL
30 mM 0.0917 mL 0.4587 mL 0.9173 mL 2.2933 mL
40 mM 0.0688 mL 0.3440 mL 0.6880 mL 1.7200 mL
50 mM 0.0550 mL 0.2752 mL 0.5504 mL 1.3760 mL
60 mM 0.0459 mL 0.2293 mL 0.4587 mL 1.1467 mL
80 mM 0.0344 mL 0.1720 mL 0.3440 mL 0.8600 mL
100 mM 0.0275 mL 0.1376 mL 0.2752 mL 0.6880 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thyrotropin-Releasing Hormone (TRH),24769-58-2TRH-OHOthersInhibitorinhibitorinhibit

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