Sapropterin dihydrochloride (Synonyms: (6R)-BH4 dihydrochloride; (6R)-Tetrahydro-L-biopterin dihydrochloride)

Name: Sapropterin dihydrochloride
Brand: MedChemExpress
SKU: HY-A0124A
Rating: 5.0 (2 reviews)

Art. -Nr.: HY-A0124A Reinheit: 99.61%: Data Sheet
COA

SDS
Handling Instructions
FAQs
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Sapropterin ((6R)-BH4; (6R)-Tetrahydro-L-biopterin) dihydrochloride is an orally active phenylalanine hydroxylase (PAH) cofactor, which is effective in reducing blood phenylalanine concentrations. Sapropterin dihydrochloride can be used in study of phenylketonuria (PKU) phenylketonuria. Sapropterin dihydrochloride can aggravate experimental autoimmune encephalomyelitis (EAE).

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Sapropterin dihydrochloride Chemische Struktur

CAS. Nr. : 69056-38-8

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10 mM * 1 mL in DMSO ready for reconstitution	Auf Lager	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	Auf Lager	Estimated Time of Arrival: December 31
Solid
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
25 mg	Auf Lager	Estimated Time of Arrival: December 31
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Based on 2 publication(s) in Google Scholar

Other Forms of Sapropterin dihydrochloride:

Tetrahydrobiopterin In-stock
Sapropterin dihydrochloride (Standard) In-stock
Sapropterin Angebot einholen

2 Publications Citing Use of MCE Sapropterin dihydrochloride

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

Human Endogenous Metabolite Microbial Metabolite

Biologische Aktivität
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Beschreibung

In Vitro

Sapropterin dihydrochloride enhances residual phenylalanine hydroxylase activity to increase phenylalanine oxidation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Sapropterin (100 mg/kg/day; oral; daily; 22 days) dihydrochloride treatment aggravates primary progressive and relapsing-remitting EAE in SJL/J mice, as evidenced by increased clinical EAE scores, elevated plasma long-chain ceramide levels, reduced linolenic acid levels, and increased spinal cord T-cell infiltration^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SJL/J (female, 10-12-week-old, primary progressive and PLP-induced-relapsing-remitting EAE)^[2]
Dosage:	100 mg/kg/day
Administration:	oral; daily; 22 days
Result:	Reached higher maximum clinical EAE scores and showed a significant increase in the area under the curve (AUC) of EAE scores. Elevated plasma levels of long-chain ceramides (C14Cer, C16Cer, C18Cer, C20Cer, C18GluCer, C24:1GluCer). Reduced plasma linolenic acid (FA18:3) levels and increased plasma levels of the endocannabinoid 1+2AG. Showed no significant effect on peripheral T-cell proliferation or subpopulations in the spleen, but increased infiltration of CD4⁺ and CD8⁺ T-cells in the spinal cord.

Klinische Studie

Molekulargewicht

314.17

Formel

C₉H₁₇Cl₂N₅O₃

CAS. Nr.

69056-38-8

Appearance

Solid

Color

White to off-white

SMILES

O=C1N=C(N)NC2=C1N[C@@H]([C@@H](O)[C@@H](O)C)CN2.[H]Cl.[H]Cl

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 100 mg/mL (318.30 mM; Need ultrasonic)

DMSO : 100 mg/mL (318.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1830 mL	15.9150 mL	31.8299 mL
5 mM	0.6366 mL	3.1830 mL	6.3660 mL
10 mM	0.3183 mL	1.5915 mL	3.1830 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (15.91 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (15.91 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (15.91 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Reinheit & Dokumentation

Purity: 99.82%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (251 KB) LCMS (109 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Levy H L, et al. Efficacy of sapropterin dihydrochloride (tetrahydrobiopterin, 6R-BH4) for reduction of phenylalanine concentration in patients with phenylketonuria: a phase III randomised placebo-controlled study[J]. The Lancet, 2007, 370(9586): 504-510. [Content Brief]

[2]. Schmitz K, et al. Sapropterin (BH4) Aggravates Autoimmune Encephalomyelitis in Mice. Neurotherapeutics. 2021 Jul;18(3):1862-1879. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1830 mL	15.9149 mL	31.8299 mL	79.5747 mL
	5 mM	0.6366 mL	3.1830 mL	6.3660 mL	15.9149 mL
	10 mM	0.3183 mL	1.5915 mL	3.1830 mL	7.9575 mL
	15 mM	0.2122 mL	1.0610 mL	2.1220 mL	5.3050 mL
	20 mM	0.1591 mL	0.7957 mL	1.5915 mL	3.9787 mL
	25 mM	0.1273 mL	0.6366 mL	1.2732 mL	3.1830 mL
	30 mM	0.1061 mL	0.5305 mL	1.0610 mL	2.6525 mL
	40 mM	0.0796 mL	0.3979 mL	0.7957 mL	1.9894 mL
	50 mM	0.0637 mL	0.3183 mL	0.6366 mL	1.5915 mL
	60 mM	0.0530 mL	0.2652 mL	0.5305 mL	1.3262 mL
	80 mM	0.0398 mL	0.1989 mL	0.3979 mL	0.9947 mL
	100 mM	0.0318 mL	0.1591 mL	0.3183 mL	0.7957 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Sapropterin dihydrochloride Related Classifications

Metabolic Disease Inflammation/Immunology Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sapropterin69056-38-8(6R)-BH4(6R)-Tetrahydro-L-biopterinEndogenous MetabolitePKUPAHphenylalanine hydroxylaseInhibitorinhibitorinhibit

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Sapropterin dihydrochloride (Synonyms: (6R)-BH4 dihydrochloride; (6R)-Tetrahydro-L-biopterin dihydrochloride)

Kundenbewertung

Other Forms of Sapropterin dihydrochloride:

Sapropterin dihydrochloride Related Antibodies

2 Publications Citing Use of MCE Sapropterin dihydrochloride

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen:

Biologische Aktivität

Reinheit & Dokumentation

Verweise

Kundenbewertung

Sapropterin dihydrochloride Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

Sapropterin dihydrochloride Related Classifications

Keywords:

Ihre kürzlich angesehenen Produkte:

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SAFETY DATA SHEET (SDS)

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