2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. APC

APC

Anaphase promoting complex

APC

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APC (Anaphase-Promoting Complex) is an E3 ubiquitin ligase that marks target cell cycle proteins for degradation by the 26S proteasome. The APC/C is a large complex of 11–13 subunit proteins, including a cullin (Apc2) and RING (Apc11) subunit much like SCF. The APC/C's main function is to trigger the transition from metaphase to anaphase by tagging specific proteins for degradation. The two proteins of most importance that get degraded in this process as substrates of the APC/C are securin and S and M cyclins. Securin releases separase, a protease, after being degraded which in turn triggers the cleavage of cohesin, the protein complex that binds sister chromatids together. During metaphase, sister chromatids are linked by intact cohesin complexes. When securin undergoes ubiquitination by the APC/C and releases separase, which degrades cohesin, sister chromatids become free to move to opposite poles for anaphase. The APC/C also targets the mitotic cyclins for degradation, resulting in the inactivation of M-CdK (mitotic cyclin-dependent kinase) complexes, promoting exit from mitosis and cytokinesis.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-124955
    proTAME
    		Inhibitor 98.20%
    proTAME, a cell-permeable proagent form of TAME, is an anaphase promoting complex/cyclosome (APC/C) inhibitor. proTAME causes cell cycle arrest in metaphase.
    proTAME
  • HY-110287
    Apcin
    		Inhibitor 99.62%
    Apcin, a ligand of Cdc20, is a potent and competitive anaphase-promoting complex/cyclosome (APC/C(Cdc20)) E3 ligase activity inhibitor. Apcin competitively inhibits APC/C-dependent ubiquitylation by binding to Cdc20 and preventing substrate recognition. Apcin occupes the D-box-binding pocket on the side face of the WD40-domain and can prolong mitosis.
    Apcin
  • HY-13255
    TAME
    		Inhibitor 99.51%
    TAME is an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC), which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest. TAME is not cell permeable.
    TAME
  • HY-130841
    Apcin-A
    		Inhibitor 98.0%
    Apcin-A is a small molecule inhibitor that selectively targets the cell division cycle protein Cdc20 and is a derivative of Apcin (HY-110287). Apcin-A competitively binds to the D-box binding pocket of Cdc20 and inhibits substrate ubiquitination mediated by the anaphase promoting complex APC/C-Cdc20. Apcin-A also blocks the binding of Cdc20 to substrates (such as securin and cyclin B1), inhibiting anaphase initiation and cell cycle exit. Apcin-A can promote or prolong mitotic slippage in coordination with p31comet under conditions of high spindle assembly checkpoint (SAC) activity. Apcin-A can be used to develop anti-mitotic drugs and overcome tumor chemotherapy resistance. Apcin-A can be used to synthesize PROTAC CP5V (HY-130257)[1][2][3].
    Apcin-A
  • HY-P1918
    Activated Protein C (390-404), human
    		98.74%
    Activated Protein C (390-404), human is a polypeptide fragment of vitamin K-dependent serine protease activated protein C (APC), which inhibits the anticoagulant activity of APC. Activated Protein C (390-404), human inhibits APC-catalyzed inactivation of factor Va.
    Activated Protein C (390-404), human
  • HY-P992193
    Anti-B7-H7 Antibody
    Anti-B7-H7 Antibody is a monoclonal antibody targeting HHLA2. It can be used in ELISA, FACS, and functional assays. For isotype controls of Anti-B7-H7 Antibody, please refer to Human IgG1 kappa, Isotype Control (HY-P99001).
    Anti-B7-H7 Antibody
  • HY-126016
    MAI-400
    		Inhibitor
    MAI-400 is an APC-Asef interaction inhibitor (Kd = 12 nM, IC50 = 250 nM). MAI-400 can be used in the research of colorectal cancer.
    MAI-400
  • HY-13255A
    TAME hydrochloride
    		Inhibitor 98.31%
    TAME hydrochloride is an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC), which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest. TAME hydrochloride is not cell permeable.
    TAME hydrochloride
  • HY-122142A
    Pyr-​Pro-​Arg-​pNA hydrochloride
    		Substrate 99.24%
    Pyr-Pro-Arg-pNA (S2366) hydrochloride is a chromogenic peptide substrate for Factor XI and Activated protein C (APC). Pyr-Pro-Arg-pNA hydrochloride can induce noncompetitive inhibition of factor XI activation through occupancy of the active site of the factor XIa-light chain. Pyr-Pro-Arg-pNA hydrochloride can be used for measurement of APC amidolytic activity.
    Pyr-​Pro-​Arg-​pNA hydrochloride
  • HY-112968
    Human protein C
    Human Protein C is activated by α-thrombin or α-thrombin/thrombomodulin complex to a serine protease, namely activated protein C (APC). Human Protein C can selectively inactivate factors Va and VIIIa and is a potent anticoagulant.
    Human protein C
  • HY-122142
    Pyr-​Pro-​Arg-​pNA
    		Substrate
    Pyr-Pro-Arg-pNA (S2366) is a chromogenic peptide substrate for Factor XI and Activated protein C (APC). Pyr-Pro-Arg-pNA can induce noncompetitive inhibition of factor XI activation through occupancy of the active site of the factor XIa-light chain. Pyr-Pro-Arg-pNA can be used for measurement of APC amidolytic activity.
    Pyr-​Pro-​Arg-​pNA
  • HY-112968A
    Bovine Protein C
    Bovine Protein C is activated by α-thrombin or α-thrombin/thrombomodulin complex to a serine protease, namely activated protein C (APC). Bovine Protein C can selectively inactivate factors Va and VIIIa and is a potent anticoagulant.
    Bovine Protein C
  • HY-179012
    CDC20/HSP90-IN-1
    		Inhibitor
    CDC20/HSP90-IN-1 (Compound 2b) is a Cdc20/Hsp90 inhibitor with a Kd of 16.2 μM for Cdc20 and a Kd of 0.241 μM for Hsp90α. CDC20/HSP90-IN-1 exerts potent antitumor activity through reducing p53-mediated Cdc20, upregulating Bim, downregulating Cyclin B1 expression, and disturbing B-Raf and AKT pathways via destroying Hsp90 chaperone function. CDC20/HSP90-IN-1 overcomes Vemurafenib (HY-12057)-induced resistant melanoma.
    CDC20/HSP90-IN-1
  • HY-P1918A
    Activated Protein C (390-404), human TFA
    Activated Protein C (390-404), human TFA, a peptide of the activated protein C (a vitamin K-dependent serine protease), potently inhibits APC anticoagulant activity.
    Activated Protein C (390-404), human TFA
  • HY-172401
    CDC20-IN-2
    		Inhibitor
    CDC20-IN-2 (14c), a Cdc20 inhibitor (KD: 7.65 μM), causes G2/M phase arrest and promotes DNA damage accumulation. CDC20-IN-2 (14c) stabilizes key substrates such as Cyclin B1 and Bim, leading to enhanced apoptosis and suppression of tumor cell proliferation and migration.
    CDC20-IN-2
  • HY-116473
    CFM-1
    		Antagonist
    CFM-1 is a small molecule antagonist of CARP-1/APC-2 binding with an EC50 value of 4.1 μM. CFM-1 induces G2M cell cycle arrest and suppresses viabilities of human breast cancer cells.
    CFM-1
  • HY-W614725
    Apcin-A monohydrochloride
    		Inhibitor
    Apcin-A monohydrochloride, an Apcin derivative, is an anaphase-promoting complex (APC) inhibitor. Apcin-A monohydrochloride interacts strongly with Cdc20, and inhibits the ubiquitination of Cdc20 substrates. Apcin-A monohydrochloride can be used to synthesize the PROTAC CP5V (HY-130257).
    Apcin-A monohydrochloride
Cat. No. Product Name / Synonyms Application Reactivity