1. Cell Cycle/DNA Damage
  2. APC
  3. Apcin

Apcin 

Cat. No.: HY-110287
Handling Instructions

Apcin, a ligand of Cdc20, is a potent and competitive anaphase-promoting complex/cyclosome (APC/C(Cdc20)) E3 ligase activity inhibitor. Apcin competitively inhibits APC/C-dependent ubiquitylation by binding to Cdc20 and preventing substrate recognition. Apcin occupes the D-box-binding pocket on the side face of the WD40-domain and can prolong mitosis.

For research use only. We do not sell to patients.

Apcin Chemical Structure

Apcin Chemical Structure

CAS No. : 300815-04-7

Size Price Stock
5 mg USD 100 Ask For Quote & Lead Time
10 mg USD 160 Ask For Quote & Lead Time
25 mg USD 320 Ask For Quote & Lead Time
50 mg USD 560 Ask For Quote & Lead Time
100 mg USD 980 Ask For Quote & Lead Time

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Apcin, a ligand of Cdc20, is a potent and competitive anaphase-promoting complex/cyclosome (APC/C(Cdc20)) E3 ligase activity inhibitor. Apcin competitively inhibits APC/C-dependent ubiquitylation by binding to Cdc20 and preventing substrate recognition. Apcin occupes the D-box-binding pocket on the side face of the WD40-domain and can prolong mitosis[1][2][3].

In Vitro

Apcin (25-50 μM; 48 hours) significantly increases MM apoptosis combining with proTAME (6, 12 μM)[3].
Apcin (25 μM; 2-14 hours) induces slippage more slowly[2].
Apcin (50-200 μM) stabilizes cycB1-NT and securin most effectively, with somewhat weaker effects against full-length cyclin B1[1].
Apcin (1.5-200 μM; 18 hours) synergizes with proTAME (a cell-permeable TAME prodrug) to prolong mitotic duration in RPE1 cells[1].

Apoptosis Analysis[3]

Cell Line: HMCLs LP-1 and RPMI-8226 cells
Concentration: 25, 50 μM
Incubation Time: 48 hours
Result: Had minor effects on multiple myeloma (MM) cells alone and significantly increased MM apoptosis combining with proTAME (6, 12 μM).

Western Blot Analysis[2]

Cell Line: HeLa cells with Nocodazole (100 nM)
Concentration: 25 μM
Incubation Time: 2-14 hours
Result: Induced slippage more slowly, as indicated by Cdc27 dephosphorylation and a reduction in cyclin B1, securin and phosphoH3 levels beginning 4-6h after mitotic entry.
Molecular Weight

438.65

Formula

C₁₃H₁₄Cl₃N₇O₄

CAS No.

300815-04-7

SMILES

O=C(OCCN1C([N+]([O-])=O)=CN=C1C)NC(NC2=NC=CC=N2)C(Cl)(Cl)Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

ApcinAPCAnaphase promoting complexanaphase-promotingcomplexcyclosomeAPC/CCdc20E3ligaseubiquitylationrecognitionD-box-bindingpocketWD40-domainmitosisInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Apcin
Cat. No.:
HY-110287
Quantity:
MCE Japan Authorized Agent: