1. Cell Cycle/DNA Damage
  2. APC
  3. TAME

TAME 

Cat. No.: HY-13255 Purity: 99.68%
Handling Instructions

TAME is an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC), which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest.

For research use only. We do not sell to patients.

TAME Chemical Structure

TAME Chemical Structure

CAS No. : 901-47-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 59 In-stock
Estimated Time of Arrival: December 31
50 mg USD 54 In-stock
Estimated Time of Arrival: December 31
100 mg USD 96 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Description

TAME is an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC), which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest[1].

IC50 & Target

Anaphase-promoting complex (APC)[1]

In Vitro

The absence of APC substrates, TAME ejects Cdc20 from the APC by promoting Cdc20 auto-ubiquitination in its N-terminal region. Cyclin B1 antagonizes TAME's effect by promoting binding of free Cdc20 to the APC and suppressing Cdc20 auto-ubiquitination[2].
TAME stabilizes cyclin B1 in Xenopus extract by two mechanisms. First, it reduces the kcat of the APCCdc20/cyclin B1 complex without affecting the Km, slowing the initial ubiquitination of unmodified cyclin B1. Second, as cyclin B1 becomes ubiquitinated, it loses its ability to promote Cdc20 binding to the APC in the presence of TAME. As a result, cyclin B1 ubiquitination terminates before reaching the threshold necessary for proteolysis[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

342.41

Formula

C₁₄H₂₂N₄O₄S

CAS No.

901-47-3

SMILES

CC1=CC=C(C=C1)S(N[[email protected]](C(OC)=O)CCCNC(N)=N)(=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
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Keywords:

TAMEAPCAnaphase promoting complexInhibitorinhibitorinhibit

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