1. Cell Cycle/DNA Damage
  2. APC
  3. TAME hydrochloride

TAME hydrochloride 

Cat. No.: HY-13255A Purity: 98.43%
COA Handling Instructions

TAME hydrochloride is an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC), which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest. TAME hydrochloride is not cell permeable.

For research use only. We do not sell to patients.

TAME hydrochloride Chemical Structure

TAME hydrochloride Chemical Structure

CAS No. : 1784-03-8

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Solution
10 mM * 1 mL in DMSO USD 59 In-stock
Estimated Time of Arrival: December 31
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100 mg USD 96 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

TAME hydrochloride is an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC), which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest. TAME hydrochloride is not cell permeable[1][2].

IC50 & Target

Anaphase-promoting complex (APC)[1]

In Vitro

The absence of APC substrates, TAME hydrochloride ejects Cdc20 from the APC by promoting Cdc20 auto-ubiquitination in its N-terminal region. Cyclin B1 antagonizes TAME hydrochloride's effect by promoting binding of free Cdc20 to the APC and suppressing Cdc20 auto-ubiquitination[2].
TAME hydrochloride stabilizes cyclin B1 in Xenopus extract by two mechanisms. First, it reduces the kcat of the APCCdc20/cyclin B1 complex without affecting the Km, slowing the initial ubiquitination of unmodified cyclin B1. Second, as cyclin B1 becomes ubiquitinated, it loses its ability to promote Cdc20 binding to the APC in the presence of TAME hydrochloride. As a result, cyclin B1 ubiquitination terminates before reaching the threshold necessary for proteolysis[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

378.87

Formula

C14H23ClN4O4S

CAS No.
SMILES

CC1=CC=C(C=C1)S(N[[email protected]](C(OC)=O)CCCNC(N)=N)(=O)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 155 mg/mL (409.11 mM; Need ultrasonic)

H2O : ≥ 100 mg/mL (263.94 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6394 mL 13.1971 mL 26.3943 mL
5 mM 0.5279 mL 2.6394 mL 5.2789 mL
10 mM 0.2639 mL 1.3197 mL 2.6394 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.58 mg/mL (6.81 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.58 mg/mL (6.81 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.58 mg/mL (6.81 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
TAME hydrochloride
Cat. No.:
HY-13255A
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