1. Cell Cycle/DNA Damage
  2. APC

TAME hydrochloride 

Cat. No.: HY-13255A Purity: 99.10%
Handling Instructions

TAME hydrochloride is an inhibitor of anaphase-promoting complex (APC), which prevents its activation by Cdc20 and Cdh1.

For research use only. We do not sell to patients.

TAME hydrochloride Chemical Structure

TAME hydrochloride Chemical Structure

CAS No. : 1784-03-8

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 59 In-stock
Estimated Time of Arrival: December 31
50 mg USD 54 In-stock
Estimated Time of Arrival: December 31
100 mg USD 96 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Other Forms of TAME hydrochloride:

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  • Technical Information

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  • References

Description

TAME hydrochloride is an inhibitor of anaphase-promoting complex (APC), which prevents its activation by Cdc20 and Cdh1.

IC50 & Target

APC [1].

In Vitro

TAME shows inhibitory effect on APC activation when added to mitotic Xenopus extract. TAME (1-200 μM) inhibits Cdc20 association with mitotic APC and also inhibits Cdh1 association with interphase APC. TAME significantly inhibits crosslinking of the IR peptide to Cdc27 and Cdc16 but only slightly reduces crosslinking to Cdc23 and Apc7 at 20 µM; TAME strongly inhibits crosslinking to all APC subunits at 200 µM. TAME also inhibits binding of wild type Cdc20 to the APC, but not binding of a ΔIR mutant[1]. TAME (200 μM) induces Cdc20 dissociation from the APC in mitotic Xenopus extract calls for APC-dependent ubiquitination. TAME induces Cdc20 dissociation from the APC by promoting Cdc20 ubiquitination, but the dissociation can be suppresses by Cyclin B1. TAME terminates cyclin B1 ubiquitination prematurely[2]. TAME interacts with ATP by β and γ phosphate and the adenine ring of ATP. TAME in combination with the Mg(II) ion accelerates the ATP hydrolysis process[3].

Solvent & Solubility
In Vitro: 

DMSO : 155 mg/mL (409.11 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6394 mL 13.1971 mL 26.3943 mL
5 mM 0.5279 mL 2.6394 mL 5.2789 mL
10 mM 0.2639 mL 1.3197 mL 2.6394 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.58 mg/mL (6.81 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.58 mg/mL (6.81 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.58 mg/mL (6.81 mM); Clear solution

References
Molecular Weight

378.87

Formula

C₁₄H₂₃ClN₄O₄S

CAS No.

1784-03-8

SMILES

CC1=CC=C(C=C1)S(N[[email protected]](C(OC)=O)CCCNC(N)=N)(=O)=O.[H]Cl

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
TAME hydrochloride
Cat. No.:
HY-13255A
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TAME hydrochloride

Cat. No.: HY-13255A