Pyr-Pro-Arg-pNA (hydrochloride)
Based on 1 Customer Validation
Pyr-Pro-Arg-pNA (S-2366) hydrochloride is a chromogenic peptidyl substrate, and a noncompetitive inhibitor that occupies the active sites of FXIa and FXIa-LC to block factor IX activation. Pyr-Pro-Arg-pNA hydrochloride functions as a hydrolyzable substrate for FXIa’s catalytic domain, human APC, murine APC, and purified plasma kallikrein.
For research use only. We do not sell to patients.
- Purity: 99.24%
- CAS No.: 127428-47-1
- Formula: C22H31ClN8O6
- Molecular Weight:538.98
-
Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Pyr-Pro-Arg-pNA (0-1.5 mM) is hydrolyzed by purified human FXIa and FXIa-LC with Km values of 225 μM and 357 μM[1].
Pyr-Pro-Arg-pNA noncompetitively inhibits human FXIa-mediated FIX activation with a Ki of 425 μM and human FXIa-LC-mediated FIX activation with a Ki of 420 μM, without altering the Km values for FIX binding to either enzyme[1].
Pyr-Pro-Arg-pNA can be hydrolyzed by purified recombinant murine activated protein C with essentially identical kinetic activity to purified human activated protein C[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 127428-47-1
-
Appearance Solid
-
Molecular Weight 538.98
-
Formula C22H31ClN8O6
-
Color White to off-white
-
Synonyms
S-2366 (hydrochloride)
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (185.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
-
Handling Instructions (2659 KB)
References
[1]. Sinha D, et al. Macromolecular substrate-binding exosites on both the heavy and light chains of factor XIa mediate the formation of the Michaelis complex required for factor IX-activation. Biochemistry. 2007 Aug 28;46(34):9830-9. [Content Brief]
[3]. Fernández JA, et al. Recombinant murine-activated protein C is neuroprotective in a murine ischemic stroke model. Blood Cells Mol Dis. 2003 May-Jun;30(3):271-6. [Content Brief]
[4]. Briseid K, et al. Part of purified human plasma kallikrein removed together with a remaining IgG fraction--immunoblot experiments and functional tests. Scand J Clin Lab Invest. 2002;62(1):21-24. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8554 mL | 9.2768 mL | 18.5536 mL | 46.3839 mL |
| 5 mM | 0.3711 mL | 1.8554 mL | 3.7107 mL | 9.2768 mL | |
| 10 mM | 0.1855 mL | 0.9277 mL | 1.8554 mL | 4.6384 mL | |
| 15 mM | 0.1237 mL | 0.6185 mL | 1.2369 mL | 3.0923 mL | |
| 20 mM | 0.0928 mL | 0.4638 mL | 0.9277 mL | 2.3192 mL | |
| 25 mM | 0.0742 mL | 0.3711 mL | 0.7421 mL | 1.8554 mL | |
| 30 mM | 0.0618 mL | 0.3092 mL | 0.6185 mL | 1.5461 mL | |
| 40 mM | 0.0464 mL | 0.2319 mL | 0.4638 mL | 1.1596 mL | |
| 50 mM | 0.0371 mL | 0.1855 mL | 0.3711 mL | 0.9277 mL | |
| 60 mM | 0.0309 mL | 0.1546 mL | 0.3092 mL | 0.7731 mL | |
| 80 mM | 0.0232 mL | 0.1160 mL | 0.2319 mL | 0.5798 mL | |
| 100 mM | 0.0186 mL | 0.0928 mL | 0.1855 mL | 0.4638 mL |
Pyr-Pro-Arg-pNA (hydrochloride) Related Classifications
HY-122142A Related Classifications
Powered by Bioz