2. Cell Cycle/DNA Damage
  3. APC
  4. proTAME

proTAME, a cell-permeable proagent form of TAME, is an anaphase promoting complex/cyclosome (APC/C) inhibitor. proTAME causes cell cycle arrest in metaphase.

For research use only. We do not sell to patients.

CAS No. : 1362911-19-0

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Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

proTAME Related Antibodies

Top Publications Citing Use of Products

    proTAME purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2025 Aug;68(8):2348-2362.  [Abstract]

    Cells treated with proTAME (10 μM; 3-12 h) were collected at the indicated times and detected with immunoblotting.

    proTAME purchased from MedChemExpress. Usage Cited in: Nature. 2024 Sep;633(8031):932-940.  [Abstract]

    Representative images of RPE-1 CDK5-as cells treated as indicated, released from RO-3306 into media containing 20μM proTAME for 2 hours and stained with α-tubulin and DAPI (upper panel). Metaphase plate width and spindle length measurements for these representative cells are shown in the table on the right. Quantification of metaphase plate width and spindle length following indicated treatments (lower panel).

    proTAME purchased from MedChemExpress. Usage Cited in: Science. 2024 Jun 7;384(6700):eadk0775.  [Abstract]

    Immunoblot analysis of APC/C target proteins securin and cyclin B1 as well as ERK activity readouts pERK and pRSK following treatment with proTAME (APC/Ci) (5-10 μM; 24 h) and/or ERKi in Pa16C cells. The results showed that proTAME was verified to prevent the reduction in protein levels of securin and cyclin B1 caused by 24-hour treatment with ERKi.

    proTAME purchased from MedChemExpress. Usage Cited in: Science. 2024 Jul 19;385(6706):eadn5529.  [Abstract]

    Preventing internal positioning reduces the risk of premature chromosome separation. 3D-reconstructed images of chromosomes (H2B-SNAP, white), NDC80-NUF2 beads (NDC80-sfGFP, green), and prematurely separated chromosomes (magenta) in proTAME (2.5 μM)-treated aged oocytes. Oocytes were monitored for premature chromosome separation by live imaging for 37 hours. In the plot, each spot corresponds to the data from one experiment. Bars indicate the percentage of oocytes that exhibited premature chromosome separation in four independent experiments. The number of oocytes is shown in parentheses. scale bars, 5 μm (for magnified images, 1.5 μm).

    proTAME purchased from MedChemExpress. Usage Cited in: FASEB J. 2024 Dec 13;38(24):e70261.  [Abstract]

    HeLa S3 cells were treated with siDESI1 #1. After 24 h, cells were incubated with 1.5 nM VCR, 5 μM proTAME, or their combination for a duration of 3 days, and a CCK‐8 assay was performed. The absorbance ratio is expressed relative to control cells (siCtrl, DMSO).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    proTAME, a cell-permeable proagent form of TAME, is an anaphase promoting complex/cyclosome (APC/C) inhibitor. proTAME causes cell cycle arrest in metaphase[1][2].

    In Vitro

    ProTAME prevents anaphase entry in mouse and bovine oocytes and also in mouse 2-cell embryos. proTAME (0-100 μM ) treatment shows dose-dependent metaphase arrest in mammalian oocytes and early cleavage embryos. And the metaphase arrest induced by this drug does not require spindle assembly checkpoint (SAC) activity[1].
    ProTAME arrest of meiosis I in mouse oocytes is due to the inhibition of APC/C. In contrast to the somatic cells, the arrest in oocytes and embryos is not reversible[1].
    proTAME (0-20 μM) dose-dependently affects morphological parameters of the spindle in oocytes and in embryos[1].
    proTAME is efficient in overcoming resistance caused by the hyperphosphorylation of CDH1 in glioblastoma cells, polo-like kinase 1 (PLK1)-based drug resistance in ovarian cancer cells and CDC20-based resistance in diffuse large B-cell lymphoma[1].
    proTAME inhibits OVCAR-3 cells growth with an IC50 of 12.5 μM[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    proTAME Related Antibodies
    Molecular Weight

    726.75

    Formula

    C34H38N4O12S
    CAS No.
    Appearance

    Oil

    Color

    White to off-white

    SMILES

    O=C(CC1=CC=CC=C1)OCOC(N/C(NC(OCOC(CC2=CC=CC=C2)=O)=O)=N/CCC[C@@H](C(OC)=O)NS(=O)(C3=CC=C(C=C3)C)=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Pure form -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (137.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3760 mL 6.8799 mL 13.7599 mL
    5 mM 0.2752 mL 1.3760 mL 2.7520 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 3.75 mg/mL (5.16 mM); Clear solution

      This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.20%

    SDS (252 KB)

    COA (249 KB) HNMR (191 KB) RP-HPLC (258 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.3760 mL 6.8799 mL 13.7599 mL 34.3997 mL
    5 mM 0.2752 mL 1.3760 mL 2.7520 mL 6.8799 mL
    10 mM 0.1376 mL 0.6880 mL 1.3760 mL 3.4400 mL
    15 mM 0.0917 mL 0.4587 mL 0.9173 mL 2.2933 mL
    20 mM 0.0688 mL 0.3440 mL 0.6880 mL 1.7200 mL
    25 mM 0.0550 mL 0.2752 mL 0.5504 mL 1.3760 mL
    30 mM 0.0459 mL 0.2293 mL 0.4587 mL 1.1467 mL
    40 mM 0.0344 mL 0.1720 mL 0.3440 mL 0.8600 mL
    50 mM 0.0275 mL 0.1376 mL 0.2752 mL 0.6880 mL
    60 mM 0.0229 mL 0.1147 mL 0.2293 mL 0.5733 mL
    80 mM 0.0172 mL 0.0860 mL 0.1720 mL 0.4300 mL
    100 mM 0.0138 mL 0.0688 mL 0.1376 mL 0.3440 mL
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    proTAME Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    proTAME1362911-19-0APCAnaphase promoting complexcell-permeablemetaphasecellcycle spindleassemblycheckpointInhibitorinhibitorinhibit

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