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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D0965
    Procion Blue HB
    Procion Blue HB (Reactive Blue 2) is a purinergic antagonist.
    Procion Blue HB
  • HY-137845
    4-Methylumbelliferyl β-D-galactopyranoside
    		99.06%
    4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer.
    4-Methylumbelliferyl β-D-galactopyranoside
  • HY-DY1003
    JC-1 (solution)
    JC-1 (CBIC2) (solution) is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. JC-1 accumulates in mitochondria in a potential dependent manner and can be used to detect the membrane potential of cells, tissues or purified mitochondria. In normal mitochondria, JC-1 aggregates in the mitochondrial matrix to form a polymer, which emits strong red fluorescence (Ex=585 nm, Em=590 nm) ; When the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria and produce green fluorescence (ex=510 nm, em= 527 nm) .
    Solvent and concentration: DMSO: 1.5 mM
    JC-1 (solution)
  • HY-152231
    BODIPY FL thalidomide
    		99.18%
    BODIPY FL thalidomide is a high-affinity and selective human cereblon (CRBN)-targeting fluorescent probe with a Kd of 3.6 nM. BODIPY FL thalidomide enables use as a probe in time-resolved fluorescence resonance energy transfer (TR-FRET) binding assays for cereblon ligands. BODIPY FL thalidomide supports development of highly sensitive, selective, stable cereblon TR-FRET binding assays (Ex/Em = 502/510 nm).
    BODIPY FL thalidomide
  • HY-W588722
    ICG-DBCO
    		98.83%
    ICG-DBCO is a near-infrared fluorescent dye that is widely used in cell dyes, biological dyes, biomolecules and particle fluorescent labeling.Ex/Em = 789/813 nm
    ICG-DBCO
  • HY-DY1014
    ATP-Red 1 (solution)
    ATP-Red 1 (solution) is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).
    Solvent and concentration: DMSO: 1 mM
    ATP-Red 1 (solution)
  • HY-114879
    DDAO
    DDAO is a promising near-infrared (NIR) red fluorescent probewith tunable excitation wavelength (600-650nm) and longemission wavelength(λem=656nm). DDAO can de desiged for detection of the activities of different enzymes such asβ-galactosidase,sulfatase, proteinphosphatase2A,carboxylesterase 2, humanalbumin andesterases.
    DDAO
  • HY-172286
    Cy7 NHS ester
    Cy7 NHS ester is a fluorescent dye that can be used for fluorescent imaging study.
    Cy7 NHS ester
  • HY-DY1061
    BODIPY 558/568 C12 (solution)
    BODIPY 558/568 C12 (solution) is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 558/568 nm.
    Solvent and concentration: DMSO: 2 mM
    BODIPY 558/568 C12 (solution)
  • HY-D1082
    5-DTAF
    5-DTAF is a fluorescein dye that directly react with polysaccharides and other alcohols in aqueous solution at pH ＞9. The maximum of the 5-DTAF emission profile is at a wavelength of 518 nm after excitation at 488 nm. 5-DTAF can be used for biomolecular labeling, protein detection, and cell imaging.
    5-DTAF
  • HY-130025
    HKOCl-3
    		99.51%
    HKOCl-3 is a highly sensitive and selective fluorescent probe for detecting hypochlorous acid.Ex: 490 nm; Em 527 nm.
    HKOCl-3
  • HY-D1422
    ER Tracker Yellow
    ER Tracker Yellow is an endoplasmic reticulum-targeted temperature-visible fluorescent probe. ER Tracker Yellow has high temperature sensitivity (3.9%/°C) and is not affected by pH and Ca2+ interference. ER Tracker Yellow can be used for real-time monitoring of local temperature changes in the ER area. ER Tracker Yellow maintains its function in fixed cells. ER Tracker Yellow is suitable for various cell lines and primary cells, such as HeLa cells, MEF cells, and other cells.
    ER Tracker Yellow
  • HY-137844
    L-Leucine-7-amido-4-methylcoumarin hydrochloride
    		99.98%
    L-Leucine-7-amido-4-methylcoumarin (Leu-AMC) hydrochloride is a bright blue fluorogenic peptidyl substrate for LAP3 (leucine aminopeptidase). L-Leucine-7-amido-4-methylcoumarin hydrochloride can be used for leucine aminopeptidase inhibition assays in vitro.
    L-Leucine-7-amido-4-methylcoumarin hydrochloride
  • HY-117401
    5-Dodecanoylaminofluorescein
    		99.32%
    5-Dodecanoylaminofluorescein is an amphipathic (amphoteric) fluorescent probe (Ex/Em = 485 nm/515-535 nm), which consists of a hydrophilic fluorescein core and a hydrophobic dodecanoyl group (C12 fatty acid chain) linked by an amide bond. 5-Dodecanoylaminofluorescein is mainly used as an interface-localized radical trapping/indicator probe. Dodecanoylaminofluorescein can be used for the determination of the antioxidant properties of emulsions, or can serve as a lipophilic drug model for the study of passive skin penetration when encapsulated in tyrosine-derived nanospheres.
    5-Dodecanoylaminofluorescein
  • HY-D2465
    CY5-Dextran (MW60-80k)
    CY5-Dextran is a fluorescent dye CY5 (HY-D0821)- labeled Dextran (HY-112624). CY5 exhibits a maximal excitation wavelength of 633 nm, and a maximal emission wavelength of 670 nm. Dextran-CY5 can be used to visualize the specific subcellular distribution of dextran at the nanoscale.
    CY5-Dextran (MW60-80k)
  • HY-135036
    4-Methylumbelliferyl butyrate
    		99.46%
    4-Methylumbelliferyl butyrate (4-MUB) is a coumarin-based fluorogenic substrate used for the identification of M. catarrhalis C4- esterase. 4-Methylumbelliferyl butyrate can converse to the blue-emissive 4-methylumbelliferone (4-MU; HY-N0187).
    4-Methylumbelliferyl butyrate
  • HY-D0249
    Sunset Yellow FCF
    		98.0%
    Sunset Yellow FCF (Orange Yellow S) is an orange azo dye with a maximum absorption wavelength of 480 nm. Sunset Yellow FCF can be used in food, cosmetics and pharmaceuticals.
    Sunset Yellow FCF
  • HY-W110898
    Nile blue chloride
    Nile blue chloride is a highly fluorescent and photostable organic dye. Nile blue chloride and fluorescein isothiocyanate (FITC) can be used to construct a ratiometric pH sensitive probe for tracking the pH of the extracellular fluid between cancer cells in realtime. Nile Blue chloride has the potential for the research of nonlinear optics.
    Nile blue chloride
  • HY-D1051
    Cy3-N3
    Cy3-N3 is a Cy3-azide fluorescent dye used to label for protein and nucleic acid. Cy3-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 548/563 nm).
    Cy3-N3
  • HY-D2096A
    Alexa fluor 647 NHS ester TEA
    Alexa fluor 647 NHS ester TEA can be used to label Alexa fluor 647 to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
    Alexa fluor 647 NHS ester TEA
Cat. No. Product Name / Synonyms Application Reactivity