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Procion Blue HB  (Synonyms: Reactive Blue 2)

Cat. No.: HY-D0965 Purity: ≥95.0%
COA Handling Instructions

Procion Blue HB (Reactive Blue 2) is a purinergic antagonist.

For research use only. We do not sell to patients.

Procion Blue HB Chemical Structure

Procion Blue HB Chemical Structure

CAS No. : 12236-82-7

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
5 mg USD 38 In-stock
10 mg USD 60 In-stock
50 mg USD 180 In-stock
100 mg USD 228 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

Procion Blue HB (Reactive Blue 2) is a purinergic antagonist.

In Vitro

Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca2+ oscillations in HeLa cells. Reactive Blue 2 enhances a Ca2+ response to histamine that is linked to the PLC cascade. Reactive Blue 2 may activate the PLC cascade in an extracellular Ca2+-dependent manner and induce Ca2+ oscillations[1]. The application of Reactive Blue 2 increases K+ secretion in a dose-dependent manner, and this increase is characterized as a peak followed by a partial relaxation to a steady-state. Reactive Blue 2 has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion[2].The anthraquinone dye reactive blue 2 is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a Ki of 8 μM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing[3]. Reactive blue 2 selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with noradrenaline, ATP and 2-methylthio ATP, but not α,β-methylene ATP, produce relaxations of the vessel. These relaxations are inhibited in the presence of reactive blue 2[4]. Reactive blue 2, at concentrations of 0.3-10 μM blocks the ATP-induced oscillation in a concentration-dependent manner[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

774.16

Formula

C29H20ClN7O11S3

CAS No.
Appearance

Solid

Color

Light brown to black

SMILES

O=S(C(C(N)=C1C2=O)=CC(NC3=CC=C(NC4=NC(Cl)=NC(NC5=CC=CC(S(=O)(O)=O)=C5)=N4)C(S(=O)(O)=O)=C3)=C1C(C6=C2C=CC=C6)=O)(O)=O.O=S(C(C(N)=C7C8=O)=CC(NC9=CC=C(NC%10=NC(Cl)=NC(NC%11=CC=C(S(=O)(O)=O)C=C%11)=N%10)C(S(=O)(O)=O)=C9)=C7C(C%12=C8C=CC=C%12)=O)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 110 mg/mL (142.09 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 10 mg/mL (12.92 mM; Need ultrasonic)

Ethanol : < 1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2917 mL 6.4586 mL 12.9172 mL
5 mM 0.2583 mL 1.2917 mL 2.5834 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.2917 mL 6.4586 mL 12.9172 mL 32.2931 mL
5 mM 0.2583 mL 1.2917 mL 2.5834 mL 6.4586 mL
10 mM 0.1292 mL 0.6459 mL 1.2917 mL 3.2293 mL
DMSO 15 mM 0.0861 mL 0.4306 mL 0.8611 mL 2.1529 mL
20 mM 0.0646 mL 0.3229 mL 0.6459 mL 1.6147 mL
25 mM 0.0517 mL 0.2583 mL 0.5167 mL 1.2917 mL
30 mM 0.0431 mL 0.2153 mL 0.4306 mL 1.0764 mL
40 mM 0.0323 mL 0.1615 mL 0.3229 mL 0.8073 mL
50 mM 0.0258 mL 0.1292 mL 0.2583 mL 0.6459 mL
60 mM 0.0215 mL 0.1076 mL 0.2153 mL 0.5382 mL
80 mM 0.0161 mL 0.0807 mL 0.1615 mL 0.4037 mL
100 mM 0.0129 mL 0.0646 mL 0.1292 mL 0.3229 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Procion Blue HB Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Procion Blue HB
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