Procion Blue HB
Based on 1 Customer Validation
Procion Blue HB (Reactive Blue 2) is a purinergic antagonist.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 97.93%
- CAS No.: 12236-82-7
- Formule: C29H20ClN7O11S3
- Masse moléculaire:774.16
-
Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
Procion Blue HB is used as an ATP receptor antagonist and induces Ca2+ oscillations in HeLa cells. Procion Blue HB enhances a Ca2+ response to histamine that is linked to the PLC cascade. Procion Blue HB may activate the PLC cascade in an extracellular Ca2+-dependent manner and induce Ca2+ oscillations[1].
The application of Procion Blue HB increases K+ secretion in a dose-dependent manner, and this increase is characterized as a peak followed by a partial relaxation to a steady-state. Procion Blue HB has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion[2].
The anthraquinone dye Procion Blue HB is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a Ki of 8 μM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing[3].
Procion Blue HB selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with noradrenaline, ATP and 2-methylthio ATP, but not α,β-methylene ATP, produce relaxations of the vessel. These relaxations are inhibited in the presence of Procion Blue HB[4].
Procion Blue HB, at concentrations of 0.3-10 μM blocks the ATP-induced oscillation in a concentration-dependent manner[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 12236-82-7
-
Appearance Solid
-
Masse moléculaire 774.16
-
Formule C29H20ClN7O11S3
-
Color Light brown to black
-
SMILES
O=S(C(C(N)=C1C2=O)=CC(NC3=CC=C(NC4=NC(Cl)=NC(NC5=CC=CC(S(=O)(O)=O)=C5)=N4)C(S(=O)(O)=O)=C3)=C1C(C6=C2C=CC=C6)=O)(O)=O.O=S(C(C(N)=C7C8=O)=CC(NC9=CC=C(NC%10=NC(Cl)=NC(NC%11=CC=C(S(=O)(O)=O)C=C%11)=N%10)C(S(=O)(O)=O)=C9)=C7C(C%12=C8C=CC=C%12)=O)(O)=O
-
Synonyms
Reactive Blue 2
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
DMSO : ≥ 110 mg/mL (142.09 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (12.92 mM; Need ultrasonic)
Ethanol : < 1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
-
Fiche technique (283 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Instruction de manipulation (2659 KB)
Références
[1]. Okuda A, et al. Reactive blue 2 induces calcium oscillations in HeLa cells. Jpn J Physiol. 2001 Jun;51(3):389-93. [Content Brief]
[2]. Lee JH, et al. Reactive blue 2, an antagonist of rat P2Y4, increases K+ secretion in rat cochlea strial marginal cells. Hear Res. 2006 Sep;219(1-2):66-73. [Content Brief]
[3]. Coughlan SJ, et al. Reactive blue 2 is a potent inhibitor of a thylakoid protein kinase. Eur J Biochem. 1991 Apr 23;197(2):467-71. [Content Brief]
[4]. Burnstock G, et al. P2-purinoceptors of two subtypes in the rabbit mesenteric artery: reactive blue 2 selectively inhibits responses mediated via the P2y-but not the P2x-purinoceptor. Br J Pharmacol. 1987 Feb;90(2):383-91. [Content Brief]
[5]. Uneyama H, et al. Suramin and reactive blue 2 are antagonists for a newly identified purinoceptor on rat megakaryocyte. Br J Pharmacol. 1994 Jan;111(1):245-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.2917 mL | 6.4586 mL | 12.9172 mL | 32.2931 mL |
| 5 mM | 0.2583 mL | 1.2917 mL | 2.5834 mL | 6.4586 mL | |
| 10 mM | 0.1292 mL | 0.6459 mL | 1.2917 mL | 3.2293 mL | |
| DMSO | 15 mM | 0.0861 mL | 0.4306 mL | 0.8611 mL | 2.1529 mL |
| 20 mM | 0.0646 mL | 0.3229 mL | 0.6459 mL | 1.6147 mL | |
| 25 mM | 0.0517 mL | 0.2583 mL | 0.5167 mL | 1.2917 mL | |
| 30 mM | 0.0431 mL | 0.2153 mL | 0.4306 mL | 1.0764 mL | |
| 40 mM | 0.0323 mL | 0.1615 mL | 0.3229 mL | 0.8073 mL | |
| 50 mM | 0.0258 mL | 0.1292 mL | 0.2583 mL | 0.6459 mL | |
| 60 mM | 0.0215 mL | 0.1076 mL | 0.2153 mL | 0.5382 mL | |
| 80 mM | 0.0161 mL | 0.0807 mL | 0.1615 mL | 0.4037 mL | |
| 100 mM | 0.0129 mL | 0.0646 mL | 0.1292 mL | 0.3229 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Procion Blue HB Related Classifications
HY-D0965 Related Classifications
Powered by Bioz