Reactive blue 2 induces calcium oscillations in HeLa cells

  • Jpn J Physiol. 2001 Jun;51(3):389-93. doi: 10.2170/jjphysiol.51.389.
A Okuda  1 ,  K Furuya ,  T Kiyohara
Affiliations
  • 1. Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585 Japan. [email protected]
PMID: 11492965 DOI: 10.2170/jjphysiol.51.389
Abstract

Reactive Blue 2 (RB), which is used as an ATP receptor antagonist, induced CA(2+) oscillations in HeLa cells. RB-induced CA(2+) oscillations were abolished in CA(2+)-free solution. RB, however, did not affect CA(2+) influx measured by Mn(2+) quenching. The PLC cascade and intracellular CA(2+) release were involved as U73122 and thapsigargin inhibited RB-induced CA(2+) oscillations. RB enhanced a CA(2+) response to histamine that is linked to the PLC cascade. RB may activate the PLC cascade in an extracellular CA(2+)-dependent manner and induce CA(2+) oscillations.

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