HKOCl-3
Based on 2 publication(s) in Google Scholar
HKOCl-3 is a highly sensitive and selective fluorescent probe for detecting hypochlorous acid.Ex: 490 nm; Em 527 nm.
For research use only. We do not sell to patients.
- Purity: 99.51%
- CAS No.: 2031170-80-4
- Formula: C26H14Cl2O6
- Molecular Weight:493.29
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) HKOCl-3
More
Biological Activity
Ex: 490 nm; Em 527 nm
HKOCl-3 (10 μM) shows an increasing florescence intensity with increasing concentration of hypochlorous acid[1].
HKOCl-3 (10 μM) shows a much higher increase in fluorescence intensity of compound HKOCl-3 than treatment with other ROS (10 μM) and RNS (100 μM) [1].
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 1-2 mM (optimized according to the experiment).
1.2 Preparation of working solution
Dilute to 1-2 μM with with PBS or serum-free medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Cells are washed with PBS
2.2 Add HKOCl-3 (1 or 2 μM) in 1.5-mL HBSS supplemented with 0.1% Chloramphenicol (HY-B0239) and 0.6 mM L-Arginine (HY-N0455).
2.3 Cells are typically incubated for 30 min at 37°C.
2.4 Fluorescence microscopy detection (Ex/Em = 488/500-530 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
527
490
Chemical Information
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CAS No. 2031170-80-4
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Appearance Solid
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Molecular Weight 493.29
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Formula C26H14Cl2O6
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Color Yellow to orange
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(OC5=CC(Cl)=C(O)C(Cl)=C5)C=C3)C6=C1C=CC=C6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Rep
Synthetic lethal CRISPR screen identifies a cancer cell-intrinsic role of PD-L1 in regulation of vulnerability to ferroptosis. [Abstract]2024 Jul 9;43(7):114477. PMID: 38985676 -
Neural Regen Res
Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke. [Abstract]2025 Mar 1;20(3):845-857. PMID: 38886957
Solvent & Solubility
DMSO : 100 mg/mL (202.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0272 mL | 10.1360 mL | 20.2721 mL | 50.6801 mL |
| 5 mM | 0.4054 mL | 2.0272 mL | 4.0544 mL | 10.1360 mL | |
| 10 mM | 0.2027 mL | 1.0136 mL | 2.0272 mL | 5.0680 mL | |
| 15 mM | 0.1351 mL | 0.6757 mL | 1.3515 mL | 3.3787 mL | |
| 20 mM | 0.1014 mL | 0.5068 mL | 1.0136 mL | 2.5340 mL | |
| 25 mM | 0.0811 mL | 0.4054 mL | 0.8109 mL | 2.0272 mL | |
| 30 mM | 0.0676 mL | 0.3379 mL | 0.6757 mL | 1.6893 mL | |
| 40 mM | 0.0507 mL | 0.2534 mL | 0.5068 mL | 1.2670 mL | |
| 50 mM | 0.0405 mL | 0.2027 mL | 0.4054 mL | 1.0136 mL | |
| 60 mM | 0.0338 mL | 0.1689 mL | 0.3379 mL | 0.8447 mL | |
| 80 mM | 0.0253 mL | 0.1267 mL | 0.2534 mL | 0.6335 mL | |
| 100 mM | 0.0203 mL | 0.1014 mL | 0.2027 mL | 0.5068 mL |
HKOCl-3 Related Classifications
HY-130025 Related Classifications
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