Cy3-N3
Based on 1 Customer Validation
Cy3-N3 is a Cy3-azide fluorescent dye used to label for protein and nucleic acid. Cy3-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 548/563 nm).
For research use only. We do not sell to patients.
- Purity: 96.39%
- CAS No.: 2322322-57-4
- Formula: C34H44N6O7S2
- Molecular Weight:712.88
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (The following is our recommended protocol; this is a guideline and should be modified according to your specific needs).
1. Dye Preparation
1.1 Preparation of Stock Solution
Prepare a 25 mM stock solution using anhydrous DMSO.
Note: It is recommended to aliquot the stock solution and store it at -20 ℃ or -80 ℃ protected from light.
1.2 Preparation of Working Solution
Dilute the stock solution with preheated PBS to prepare a 25 μM working solution.
Note: Please adjust the working solution concentration according to your actual situation, and prepare it fresh before use.
2. Click Reaction
2.1 Collect cells and lyse them, retaining the protein in the cell lysate (1 mg/mL).
2.2 Add Cy5-N3 (final concentration 25 μM), TCEP (final concentration 1 mM), and TBTA (final concentration 100 μM).
2.3 Vortex mix
2.4 Add CuSO4 (final concentration 1 mM) and incubate by rotation at room temperature for 1-2 hours.
2.5 Precipitate using a protein precipitation kit to remove excess click reaction reagent.
2.6 Separate and detect by gel electrophoresis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
570
550
Chemical Information
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CAS No. 2322322-57-4
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Appearance Solid
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Molecular Weight 712.88
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Formula C34H44N6O7S2
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Color Brown to dark brown
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SMILES
CC1(C)C(/C=C/C=C2N(CCCCCC(NCCCN=[N+]=[N-])=O)C(C=CC(S(=O)(O)=O)=C3)=C3C\2(C)C)=[N+](CC)C4=CC=C([S-](=O)(=O)=O)C=C41
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (140.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4028 mL | 7.0138 mL | 14.0276 mL | 35.0690 mL |
| 5 mM | 0.2806 mL | 1.4028 mL | 2.8055 mL | 7.0138 mL | |
| 10 mM | 0.1403 mL | 0.7014 mL | 1.4028 mL | 3.5069 mL | |
| 15 mM | 0.0935 mL | 0.4676 mL | 0.9352 mL | 2.3379 mL | |
| 20 mM | 0.0701 mL | 0.3507 mL | 0.7014 mL | 1.7535 mL | |
| 25 mM | 0.0561 mL | 0.2806 mL | 0.5611 mL | 1.4028 mL | |
| 30 mM | 0.0468 mL | 0.2338 mL | 0.4676 mL | 1.1690 mL | |
| 40 mM | 0.0351 mL | 0.1753 mL | 0.3507 mL | 0.8767 mL | |
| 50 mM | 0.0281 mL | 0.1403 mL | 0.2806 mL | 0.7014 mL | |
| 60 mM | 0.0234 mL | 0.1169 mL | 0.2338 mL | 0.5845 mL | |
| 80 mM | 0.0175 mL | 0.0877 mL | 0.1753 mL | 0.4384 mL | |
| 100 mM | 0.0140 mL | 0.0701 mL | 0.1403 mL | 0.3507 mL |
Cy3-N3 Related Classifications
HY-D1051 Related Classifications
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