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  3. Fluorescent Dye
  4. Cy3-N3

Cy3-N3 is a Cy3-azide fluorescent dye used to label for protein and nucleic acid. Cy3-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 548/563 nm).

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Cy3-N3

Cy3-N3 Chemical Structure

CAS No. : 2322322-57-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
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Based on 1 publication(s) in Google Scholar

Cy3-N3 Related Antibodies

Top Publications Citing Use of Products

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  • Purity & Documentation

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Description

Cy3-N3 is a Cy3-azide fluorescent dye used to label for protein and nucleic acid. Cy3-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 548/563 nm).

In Vitro

Guidelines (The following is our recommended protocol; this is a guideline and should be modified according to your specific needs).
1. Dye Preparation
1.1 Preparation of Stock Solution Prepare a 25 mM stock solution using anhydrous DMSO.
Note: It is recommended to aliquot the stock solution and store it at -20 ℃ or -80 ℃ protected from light.
1.2 Preparation of Working Solution Dilute the stock solution with preheated PBS to prepare a 25 μM working solution.
Note: Please adjust the working solution concentration according to your actual situation, and prepare it fresh before use.
2. Click Reaction
2.1 Collect cells and lyse them, retaining the protein in the cell lysate (1 mg/mL).
2.2 Add Cy5-N3 (final concentration 25 μM), TCEP (final concentration 1 mM), and TBTA (final concentration 100 μM).
2.3 Vortex mix
2.4 Add CuSO4 (final concentration 1 mM) and incubate by rotation at room temperature for 1-2 hours.
2.5 Precipitate using a protein precipitation kit to remove excess click reaction reagent.
2.6 Separate and detect by gel electrophoresis.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cy3-N3 Related Antibodies
Molecular Weight

712.88

Formula

C34H44N6O7S2
CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

570
Excitation (Ex)

550
SMILES

CC1(C)C(/C=C/C=C2N(CCCCCC(NCCCN=[N+]=[N-])=O)C(C=CC(S(=O)(O)=O)=C3)=C3C\2(C)C)=[N+](CC)C4=CC=C([S-](=O)(=O)=O)C=C41
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (140.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4028 mL 7.0138 mL 14.0276 mL
5 mM 0.2806 mL 1.4028 mL 2.8055 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.39%

SDS (251 KB)

COA (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4028 mL 7.0138 mL 14.0276 mL 35.0690 mL
5 mM 0.2806 mL 1.4028 mL 2.8055 mL 7.0138 mL
10 mM 0.1403 mL 0.7014 mL 1.4028 mL 3.5069 mL
15 mM 0.0935 mL 0.4676 mL 0.9352 mL 2.3379 mL
20 mM 0.0701 mL 0.3507 mL 0.7014 mL 1.7535 mL
25 mM 0.0561 mL 0.2806 mL 0.5611 mL 1.4028 mL
30 mM 0.0468 mL 0.2338 mL 0.4676 mL 1.1690 mL
40 mM 0.0351 mL 0.1753 mL 0.3507 mL 0.8767 mL
50 mM 0.0281 mL 0.1403 mL 0.2806 mL 0.7014 mL
60 mM 0.0234 mL 0.1169 mL 0.2338 mL 0.5845 mL
80 mM 0.0175 mL 0.0877 mL 0.1753 mL 0.4384 mL
100 mM 0.0140 mL 0.0701 mL 0.1403 mL 0.3507 mL
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Cy3-N3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cy3-N32322322-57-4Fluorescent DyeInhibitorinhibitorinhibit

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