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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-W800703
    Alexa Fluor 594 azide
    		99.71%
    Alexa Fluor 594 azide is an azide-modified fluorescent dye and also a FRET acceptor reactant. Owing to its negligible direct absorption under 488 nm excitation light, excellent photostability, and the ability of its azide group to participate in the CuAAC reaction, Alexa Fluor 594 azide can be paired with a donor dye for single-molecule imaging via TIRFM, thereby enabling the visual monitoring of CuNP-catalyzed click reactions.
    Alexa Fluor 594 azide
  • HY-W753658
    Coppersensor-3
    Coppersensor-3 (CS3) is a bright small-molecule fluorescent probe for selective Cu(I) detection. Coppersensor-3 is selective and sensitive enough to image labile copper pools in living cells at endogenous, basal levels. Coppersensor-3 can be used for the cell biology of copper research.
    Coppersensor-3
  • HY-D0925B
    Cy5.5-SE TEA
    Cy5.5-SE TEA (Cyanine5.5 NHS ester TEA) is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance. Storage: protect from light.
    Cy5.5-SE TEA
  • HY-108715A
    RT-AM
    		98.01%
    RT-AM is a pro-agent real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.
    RT-AM
  • HY-120115
    PARPi-FL
    		99.77%
    PARPi-FL (Olaparib-bodipy FL) is a small-molecule fluorescent inhibitor of PARP1 that can specifically bind to PARP1. PARPi-FL can be used as a fluorescent imaging agent for tumor detection, diagnosis, and surgical guidance.
    PARPi-FL
  • HY-D0127
    Merocyanin 540
    		99.37%
    Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm).
    Merocyanin 540
  • HY-D0982
    Zinquin
    		99.77%
    Zinquin is a cell-impermeant fluorescent sensor and used to observe reactive Zn2+. λex/λem=368/490 nm.
    Zinquin
  • HY-D0099
    Fluorescein-5-thiosemicarbazide
    Fluorescein-5-thiosemicarbazide is a amine containing fluorescent probe that can be used for labeling saccharides and protein carbonyl derivatives.
    Fluorescein-5-thiosemicarbazide
  • HY-W034053
    Ir[p-F(Me)ppy]2(dtbbpy)PF6
    Ir[p-F (Me) ppy]2 (dtbbpy) PF6 is a cyclometalated iridium (III) complex. Ir[p-F (Me) ppy]2 (dtbbpy) PF6 absorbs visible light (460 nm) to form a long-lived charge-separated excited state. Ir[p-F (Me) ppy]2 (dtbbpy) PF6 is applicable to visible light-mediated photocatalytic organic transformation reactions.
    Ir[p-F(Me)ppy]2(dtbbpy)PF6
  • HY-D2096
    Alexa fluor 647 NHS ester
    Alexa fluor 647 NHS ester can be used to label Alexa fluor 647 to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
    Alexa fluor 647 NHS ester
  • HY-W088089
    ICG-carboxylic acid
    		99.85%
    ICG-carboxylic acid is near-infrared (NIR) fluorescent probe. ICG is a fluorescent dye used in medical diagnostics. ICG has absorption peaking at 800 nm and can absorb the near IR laser energy and release heat in the dyed tissue (Ex/Em = 785/810 nm).
    ICG-carboxylic acid
  • HY-125962
    X-34
    X-34 is a lipophilic and bright yellow-green fluorescent derivative of Congo red (HY-D0236). X-34 can be used to stain neuritic and diffuse plaques, neurofibrillary tangles (NFTs), neuropil threads, and cerebrovascular amyloid in the brain. X-34 can be used for research of Alzheimer’s disease.
    X-34
  • HY-D1151
    HKOH-1
    		98.13%
    HKOH-1 is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively.
    HKOH-1
  • HY-D1080
    EDANS
    		99.90%
    EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).
    EDANS
  • HY-D1148
    HKGreen-4I
    		99.86%
    HKGreen-4I is a highly sensitive green fluorescent probe for the specific detection of ONOO- in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively.
    HKGreen-4I
  • HY-W073524
    DFAME
    		99.20%
    DFAME is a red fluorophore (Ex = 508 nm, Em = 641 nm). DFAME binds to Beetroot and Corn (dimeric fluorescent RNA aptamers) to form Beetroot-DFAME (Kd = 460 nM) and Corn-DFAME (Kd = 3600 nM). Beetroot-DFAME and Corn-DFAME can be used to form RNA assemblies in living cells. Creating RNA assemblies can be used for the study of RNA Nanostructures. DNA/RNA Nanostructures would be useful in cell and gene therapy (CGT) research.
    DFAME
  • HY-D1250
    Mito-TRFS
    		99.11%
    Mito-TRFS, the first off-on probe, is used to image the mitochondrial thioredoxin reductase (TrxR2) in live cells.
    Mito-TRFS
  • HY-D1871
    Cy3 maleimide chloride
    		99.25%
    Cy3 maleimide chloride is a dye derivative of Cyanine 3 (Cy3) (HY-D0822) containing maleimide functional groups. Cy3 is a fluorescent dye with a fluorescence spectrum typically in the green to orange wavelength range. The alkyne functional group of Cy3 maleimide chloride can undergo a "thiol-acrylamide" reaction with molecules containing sulfur-oxygen functional groups to form covalent bonds. Cy3 maleimide chloride can bind to biological molecules such as proteins and antibodies to track their location and dynamic changes in biological samples.
    Cy3 maleimide chloride
  • HY-108667
    TNP-ATP triethylammonium
    		98.70%
    TNP-ATP triethylammonium is a P2X receptor antagonist with an IC50 of 0.010 μM for P2X3 and an IC50 of 0.062 μM for P2X2/3. TNP-ATP triethylammonium acts as an inhibitor of CheA autophosphorylation, with a Ki of 0.7 µM. TNP-ATP triethylammonium blocks the functional activation of P2X1-7 receptors. TNP-ATP triethylammonium attenuates hypoxia-induced IL-1β expression and release. TNP-ATP triethylammonium alleviates visceral pain, and improves hypoxia-induced cognitive impairment, insufficient myelination and neuroinflammation. Binding of TNP-ATP triethylammonium to CheA enhances the fluorescence of the TNP group. TNP-ATP triethylammonium can be used in studies related to visceral pain. NP-ATP triethylammonium can be used in studies related to hypoxia-induced insufficient myelination and cognitive decline.
    TNP-ATP triethylammonium
  • HY-130013
    HKYellow-AM (6/12-mixture)
    		99.36%
    HKYellow-AM (6/12-mixture) is a yellow fluorescent probe that can detect ONOO- in living cells and tissues with high selectivity and sensitivity without cytotoxicity.
    HKYellow-AM (6/12-mixture)
Cat. No. Product Name / Synonyms Application Reactivity