RT-AM
Based on 6 publication(s) in Google Scholar
RT-AM is a pro-agent real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.
For research use only. We do not sell to patients.
- Purity: 98.84%
- CAS No.: 2280796-94-1
- Formula: C26H25N3O11
- Molecular Weight:555.49
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) RT-AM
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Biological Activity
To enhance the cell permeability of Real Thiol, the carboxylic acid groups is converted to acetoxymethyl (AM) esters, which are readily hydrolysed by esterases to regenerate Real Thiol inside cells. For cells treated with the RT-AM, real-time ratiometric images of the rapid changes of intracellular GSH concentrations in single cells can be generated by dividing the fluorescence intensity values for the 405 nm channel by the 488 nm channel at each corresponding pixel[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2280796-94-1
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Appearance Solid
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Molecular Weight 555.49
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Formula C26H25N3O11
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Color Pink to red
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SMILES
O=C1OC2=CC(N3CCC3)=CC=C2C=C1/C=C(C#N)/C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (6)
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Journal Impact Factor
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Most Recent
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Environ Sci Technol
2025 Aug 12;59(31):16332-16343. PMID: 40754948 -
Environ Sci Technol
Cell-Type-Dependent Dissolution of CuO Nanoparticles and Efflux of Cu Ions following Cellular Internalization. [Abstract]2022 Sep 6;56(17):12404-12415. PMID: 35946305 -
J Hazard Mater
2024 Sep 5:476:135003. PMID: 38917627 -
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Environ Pollut
2022 Jan 1;292(Pt A):118296. PMID: 34627961 -
Solvent & Solubility
DMSO : 25 mg/mL (45.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
The HeLa cell line is grown in DMEM media supplemented with 10% FBS and 1% 1003 Pen Strep. Cells are cultured under a controlled atmosphere (37°C, 5% CO2). Glass bottom dishes are used for cell culture due to confocal scanning requirements. Cells are treated with RT-AM (1 μM with 1% DMSO in DMEM) for 10-15 min before imaging. Confocal images are acquired with 405 nm laser/418–495 nm filter and 488 nm laser/499–615 nm filter. All the microscope settings are kept consistent in each[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8002 mL | 9.0011 mL | 18.0021 mL | 45.0053 mL |
| 5 mM | 0.3600 mL | 1.8002 mL | 3.6004 mL | 9.0011 mL | |
| 10 mM | 0.1800 mL | 0.9001 mL | 1.8002 mL | 4.5005 mL | |
| 15 mM | 0.1200 mL | 0.6001 mL | 1.2001 mL | 3.0004 mL | |
| 20 mM | 0.0900 mL | 0.4501 mL | 0.9001 mL | 2.2503 mL | |
| 25 mM | 0.0720 mL | 0.3600 mL | 0.7201 mL | 1.8002 mL | |
| 30 mM | 0.0600 mL | 0.3000 mL | 0.6001 mL | 1.5002 mL | |
| 40 mM | 0.0450 mL | 0.2250 mL | 0.4501 mL | 1.1251 mL |
RT-AM Related Classifications
HY-108715A Related Classifications
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