Alexa Fluor 594 azide

As a FRET acceptor dye, Alexa Fluor 594 azide is used to verify the heterogeneous catalytic properties of CuAAC click reactions catalyzed by copper nanoparticles (CuNP)^[1].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation: Prepare an aqueous solution of 100 pM Alexa Fluor 594 azide, and mix it with an equal concentration of Alexa Fluor 488 alkyne (FRET donor) and 1 nM triethylamine, where triethylamine serves to accelerate the reaction and protect the catalyst.
2. Reaction system setup: Pump the above mixed solution into a flow cell reactor at a flow rate of 1 mL/h. The reactor substrate is a circular glass coverslip modified with APTES and spin-coated with CuNP, and the coverslip is placed on the objective lens of an Olympus TIRF microscope.
3. Single-molecule imaging detection: Irradiate the reaction system with a 488 nm laser. After removing residual donor emission light from the emitted light using a 550 long-pass filter, an EM-CCD records the signals at a rate of 10 frames/s. The reaction is monitored via bright fluorescence burst signals against a dark background.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.