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EDANS (Synonyms: 1,5-EDANS)

Cat. No.: HY-D1080
Handling Instructions

EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).

For research use only. We do not sell to patients.

EDANS Chemical Structure

EDANS Chemical Structure

CAS No. : 50402-56-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET)[1].

In Vitro

The peptide sequence of EDANS is derived from a natural processing site for HIV-1 PR[1].
Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that was linearly related to the extent of substrate hydrolysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

266.32

Formula

C₁₂H₁₄N₂O₃S

CAS No.

50402-56-7

SMILES

O=S(C1=C2C=CC=C(NCCN)C2=CC=C1)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 13.89 mg/mL (52.16 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7549 mL 18.7744 mL 37.5488 mL
5 mM 0.7510 mL 3.7549 mL 7.5098 mL
10 mM 0.3755 mL 1.8774 mL 3.7549 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

EDANS1,5-EDANSOthersfluorogenic substrateretroviral proteaseRETresonance energy transferInhibitorinhibitorinhibit

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EDANS
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HY-D1080
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