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  4. EDANS

EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).

For research use only. We do not sell to patients.

EDANS

EDANS Chemical Structure

CAS No. : 50402-56-7

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Based on 3 publication(s) in Google Scholar

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EDANS Related Antibodies

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Description

EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET)[1].

In Vitro

The peptide sequence of EDANS is derived from a natural processing site for HIV-1 PR[1].
Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that was linearly related to the extent of substrate hydrolysis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EDANS Related Antibodies
Molecular Weight

266.32

Formula

C12H14N2O3S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=S(C1=C2C=CC=C(NCCN)C2=CC=C1)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 5.83 mg/mL (21.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7549 mL 18.7744 mL 37.5488 mL
5 mM 0.7510 mL 3.7549 mL 7.5098 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.90%

SDS (393 KB)

COA (249 KB) HNMR (251 KB) RP-HPLC (251 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7549 mL 18.7744 mL 37.5488 mL 93.8720 mL
5 mM 0.7510 mL 3.7549 mL 7.5098 mL 18.7744 mL
10 mM 0.3755 mL 1.8774 mL 3.7549 mL 9.3872 mL
15 mM 0.2503 mL 1.2516 mL 2.5033 mL 6.2581 mL
20 mM 0.1877 mL 0.9387 mL 1.8774 mL 4.6936 mL
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EDANS Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EDANS50402-56-71,5-EDANSFluorescent Dyefluorogenic substrateretroviral proteaseRETresonance energy transferInhibitorinhibitorinhibit

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