1. Immunology/Inflammation Others NF-κB Metabolic Enzyme/Protease
  2. Fluorescent Dye Reactive Oxygen Species
  3. HKOH-1

HKOH-1 is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively.

For research use only. We do not sell to patients.

HKOH-1 Chemical Structure

HKOH-1 Chemical Structure

CAS No. : 2031170-96-2

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1 mg USD 300 In-stock
5 mg USD 900 In-stock
10 mg USD 1550 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

HKOH-1 is a highly sensitive green fluorescent probe for the specific detection of ·OH in living cells with a maximum excitation wavelength and emission wavelength of 500 nm and 520 nm, respectively[1].

In Vitro

1. Preparation of HKOH-1 working solution
1.1 Preparation of the stock solution
Dissolve 1 mg HKOH-1 in 135 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HKOH-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of HKOH-1 working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

745.08

Formula

C26H12Cl2I2O6

CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

520

Excitation (Ex)

500

SMILES

O=C1OC(C(C2=C3)=CC(Cl)=C3O)(C4=CC=CC=C41)C(C(O2)=C5)=CC(Cl)=C5OC(C=C6I)=CC(I)=C6O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 180 mg/mL (241.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3421 mL 6.7107 mL 13.4214 mL
5 mM 0.2684 mL 1.3421 mL 2.6843 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3421 mL 6.7107 mL 13.4214 mL 33.5534 mL
5 mM 0.2684 mL 1.3421 mL 2.6843 mL 6.7107 mL
10 mM 0.1342 mL 0.6711 mL 1.3421 mL 3.3553 mL
15 mM 0.0895 mL 0.4474 mL 0.8948 mL 2.2369 mL
20 mM 0.0671 mL 0.3355 mL 0.6711 mL 1.6777 mL
25 mM 0.0537 mL 0.2684 mL 0.5369 mL 1.3421 mL
30 mM 0.0447 mL 0.2237 mL 0.4474 mL 1.1184 mL
40 mM 0.0336 mL 0.1678 mL 0.3355 mL 0.8388 mL
50 mM 0.0268 mL 0.1342 mL 0.2684 mL 0.6711 mL
60 mM 0.0224 mL 0.1118 mL 0.2237 mL 0.5592 mL
80 mM 0.0168 mL 0.0839 mL 0.1678 mL 0.4194 mL
100 mM 0.0134 mL 0.0671 mL 0.1342 mL 0.3355 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
HKOH-1
Cat. No.:
HY-D1151
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