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  3. Merocyanin 540

Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm).

For research use only. We do not sell to patients.

Merocyanin 540 Chemical Structure

Merocyanin 540 Chemical Structure

CAS No. : 62796-23-0

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5 mg USD 30 In-stock
10 mg USD 50 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm)[1][2].

In Vitro

Guidelines[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Wash monolayer cultured cells with isotonic saline with or without 1-5 mM CaCl2 or iso-osmotic sucrose (0.25 M) solutions buffered with 5 mM Tris-Cl (pH 7.4).
2. Expose cells to Merocyanin 540 (1.6 x 10-5 M) in the appropriate buffer for 10 min at room temperature under ambient lighting conditions.
3. Wash extensively and examine by fluorescence microscopy.
4. For protozoa, place 0.05 mL aliquots of washed cultures containing 100-200 organisms on a microscope slide.
5. Add Merocyanin 540 to a final concentration of 1.0-10 μg/mL.
6. Examine cells by fluorescence microscopy.
7. Incubate single cell suspensions or tissue slices with Merocyanin 540 (1.75-5.25 x 10-5 M) and examine by fluorescence microscopy as described for attached cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

569.67

Formula

C26H32N3NaO6S2

CAS No.
Appearance

Solid

Color

Dark purple to black

Emission (Em)

580

Excitation (Ex)

540

SMILES

O=C1/C(C(N(CCCC)C(N1CCCC)=S)=O)=C/C=C/C=C2OC3=C(N/2CCCS(=O)(O[Na])=O)C=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (219.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7554 mL 8.7770 mL 17.5540 mL
5 mM 0.3511 mL 1.7554 mL 3.5108 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.89%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7554 mL 8.7770 mL 17.5540 mL 43.8851 mL
5 mM 0.3511 mL 1.7554 mL 3.5108 mL 8.7770 mL
10 mM 0.1755 mL 0.8777 mL 1.7554 mL 4.3885 mL
15 mM 0.1170 mL 0.5851 mL 1.1703 mL 2.9257 mL
20 mM 0.0878 mL 0.4389 mL 0.8777 mL 2.1943 mL
25 mM 0.0702 mL 0.3511 mL 0.7022 mL 1.7554 mL
30 mM 0.0585 mL 0.2926 mL 0.5851 mL 1.4628 mL
40 mM 0.0439 mL 0.2194 mL 0.4389 mL 1.0971 mL
50 mM 0.0351 mL 0.1755 mL 0.3511 mL 0.8777 mL
60 mM 0.0293 mL 0.1463 mL 0.2926 mL 0.7314 mL
80 mM 0.0219 mL 0.1097 mL 0.2194 mL 0.5486 mL
100 mM 0.0176 mL 0.0878 mL 0.1755 mL 0.4389 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Merocyanin 540
Cat. No.:
HY-D0127
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