2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-15935C
    X-Gluc sodium
    		98.0%
    X-Gluc sodium is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).
    X-Gluc sodium
  • HY-D2801B
    FITC-PEG3400-NHS
    		98.0%
    FITC-PEG3400-NHS is a fluorescent dye composed of polyethylene glycol (PEG), FITC (HY-66019) and a hydroxysuccinimide (NHS) group. The NHS group can react with amine-containing groups. FITC-PEG3400-NHS can be used for fluorescent labeling and imaging.
    FITC-PEG3400-NHS
  • HY-137337
    Suc-Ala-Ala-Phe-AMC
    		99.89%
    Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays.
    Suc-Ala-Ala-Phe-AMC
  • HY-D1677
    5(6)-Carboxynaphthofluorescein
    5(6)-Carboxynaphthofluorescein is a pH-dependent fluorophore. 5(6)-Carboxynaphthofluorescein shows good sensitivity in an alkaline pH range and it can be exploited in the construction of fiber-optic pH sensors. 5(6)-Carboxynaphthofluorescein can be used as a fluorescent pH indicator (Ex/Em=593/668 nm).
    5(6)-Carboxynaphthofluorescein
  • HY-D1251
    TRFS-red
    		98.86%
    TRFS-red, a red fluorescence emission off-on probe, is selective for thioredoxin reductase (TrxR). TRFS-red exhibits high response rate and sensitivity. TRFS-red can be used for imaging live cells.
    TRFS-red
  • HY-P4417
    Ac-IEPD-AMC
    		99.34%
    Ac-IEPD-AMC is a fluorogenic substrate for the determination of protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal.
    Ac-IEPD-AMC
  • HY-P4920
    Mca-SEVNLDAEFK(Dnp)-NH2
    		98.82%
    Mca-SEVNLDAEFK(Dnp)-NH2 contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence, such as can be used to measure the activity of BACE-1.
    Mca-SEVNLDAEFK(Dnp)-NH2
  • HY-123495
    CRANAD-28
    		98.03%
    CRANAD-28 is a robust fluorescent compound for visualization of amyloid beta plaques.
    CRANAD-28
  • HY-D1896
    Chloride Ionophore IV
    		98.0%
    Chloride Ionophore IV is a thiourea type hydrogen bonding-based receptor. Chloride Ionophore IV is a chloride ionophore.
    Chloride Ionophore IV
  • HY-P0082F
    Glucagon (1-29), FAM-labeled
    Glucagon (1-29), FAM-labeled is a biological active peptide. (FAM labeled HY-P0082)
    Glucagon (1-29), FAM-labeled
  • HY-D2893K
    RB-PEG8000-RB
    RB-PEG8000-RB (Rhodamine B-PEG8000-Rhodamine B) a fluorescent dye composed of two Rhodamine B (HY-Y0016) and PEG. RB-PEG8000-RB can be used for fluorescent labeling and imaging (Ex/Em=546/610 nm).
    RB-PEG8000-RB
  • HY-P11329
    GFP11
    		98.57%
    GFP11 is a protein fusion tag consisting of 16 amino acids. When GFP11 and GFP1-10 are sufficiently close in physical space, they undergo spontaneous, irreversible complementation to reassemble into an intact, functional GFP protein that emits green fluorescence. GFP11 can be used to form a split GFP system for high-throughput biotechnology and flow cytometry applications.
    GFP11
  • HY-D1174
    Rhodamine 700
    		98.50%
    Rhodamine 700, a Lambdachrome laser dye, is one of the few rhodamine dyes with near infrared fluorescence. Rhodamine 700 can be used for mitochondrial staining.
    Rhodamine 700
  • HY-141058
    Cy5-PEG3-azide
    		99.47%
    Cy5-PEG3-azide is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. Cy5-PEG3-azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cy5-PEG3-azide
  • HY-D1342
    Coumberol
    		99.99%
    Coumberol is a fluorescent substrate of AKR1C3 protein. Coumberol can be used for the research of AKR1C3.
    Coumberol
  • HY-D0853
    DiAzKs
    		99.93%
    DiAzKs (H-L-Photo-lysine) is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs acts as a UV light-activated photo-crosslinking probe.
    DiAzKs
  • HY-124197
    Coumarin hydrazine
    Coumarin hydrazine is a fluorescent chemical probe (λex=420–450/λem=468nm) to label cellular protein- and lipid-bound carbonyls.
    Coumarin hydrazine
  • HY-P5367
    5-FAM-PMDM6
    5-FAM-PMDM6 (PMDM6-F) is a biological active peptide. (PMDM6-F is a fluorescent-labeled probe for MDM2-binding assay.)
    5-FAM-PMDM6
  • HY-W591768
    (E)-4-(4-(Dimethylamino)styryl)-1-methylpyridin-1-ium iodide
    		99.66%
    (E)-4-(4-(Dimethylamino)styryl)-1-methylpyridin-1-ium (iodide) (Py-NMe2) is a styryl-based dye for fluorescence and CD-based sensing of various ds-DNA/RNA sequence. (maxλAbs = 450 nm, maxλEm = 615 nm).
    (E)-4-(4-(Dimethylamino)styryl)-1-methylpyridin-1-ium iodide
  • HY-168378
    D-Val-Leu-Lys-pNA
    		99.9%
    D-Val-Leu-Lys-pNA (S-2251) is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity.
    D-Val-Leu-Lys-pNA
Cat. No. Product Name / Synonyms Application Reactivity