D-Val-Leu-Lys-pNA
Based on 1 Customer Validation
D-Val-Leu-Lys-pNA (S-2251) is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity.
For research use only. We do not sell to patients.
- Purity: 99.9%
- CAS No.: 63589-93-5
- Formula: C23H38N6O5
- Molecular Weight:478.59
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
D-Val-Leu-Lys-pNA (1 mM; 30 min at 37°C) was the substrate against which purified DFE27 serine protease from Bacillus subtilis DC27 exhibits the highest amidolytic activity, with a hydrolysis rate of 225.291 mmol·min−1·mL−1[1].
D-Val-Leu-Lys-pNA (0.16 mM) is hydrolyzed by Lys-gingipain from Porphyromonas gingivalis (strain H66) with a Km of 0.2 mM, Vmax of 133 mmol/min, and Vmax/Km ratio of 665[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 63589-93-5
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Appearance Solid
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Molecular Weight 478.59
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Formula C23H38N6O5
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Color White to light yellow
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SMILES
CC(C)[C@@H](N)C(N[C@@H](CC(C)C)C(N[C@@H](CCCCN)C(NC1=CC=C(C=C1)[N+]([O-])=O)=O)=O)=O
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Synonyms
S-2251
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (104.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hu Y, et al. Purification and characterization of a novel, highly potent fibrinolytic enzyme from Bacillus subtilis DC27 screened from Douchi, a traditional Chinese fermented soybean food. Sci Rep. 2019;9(1):9235. Published 2019 Jun 25. [Content Brief]
[2]. Pike R, et al. Lysine- and arginine-specific proteinases from Porphyromonas gingivalis. Isolation, characterization, and evidence for the existence of complexes with hemagglutinins. J Biol Chem. 1994 Jan 7;269(1):406-11. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0895 mL | 10.4474 mL | 20.8947 mL | 52.2368 mL |
| 5 mM | 0.4179 mL | 2.0895 mL | 4.1789 mL | 10.4474 mL | |
| 10 mM | 0.2089 mL | 1.0447 mL | 2.0895 mL | 5.2237 mL | |
| 15 mM | 0.1393 mL | 0.6965 mL | 1.3930 mL | 3.4825 mL | |
| 20 mM | 0.1045 mL | 0.5224 mL | 1.0447 mL | 2.6118 mL | |
| 25 mM | 0.0836 mL | 0.4179 mL | 0.8358 mL | 2.0895 mL | |
| 30 mM | 0.0696 mL | 0.3482 mL | 0.6965 mL | 1.7412 mL | |
| 40 mM | 0.0522 mL | 0.2612 mL | 0.5224 mL | 1.3059 mL | |
| 50 mM | 0.0418 mL | 0.2089 mL | 0.4179 mL | 1.0447 mL | |
| 60 mM | 0.0348 mL | 0.1741 mL | 0.3482 mL | 0.8706 mL | |
| 80 mM | 0.0261 mL | 0.1306 mL | 0.2612 mL | 0.6530 mL | |
| 100 mM | 0.0209 mL | 0.1045 mL | 0.2089 mL | 0.5224 mL |
D-Val-Leu-Lys-pNA Related Classifications
HY-168378 Related Classifications
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