D-Val-Leu-Lys-pNA (Synonyms: S-2251)

Cat. No.: HY-168378 Purity: 99.9%: Data Sheet
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D-Val-Leu-Lys-pNA (S-2251) is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity.

For research use only. We do not sell to patients.

D-Val-Leu-Lys-pNA Chemical Structure

CAS No. : 63589-93-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Other Forms of D-Val-Leu-Lys-pNA:

D-Val-Leu-Lys-pNA dihydrochloride In-stock
D-Val-Leu-Lys-pNA acetate Get quote

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Description

In Vitro

D-Val-Leu-Lys-pNA (1 mM; 30 min at 37°C) was the substrate against which purified DFE27 serine protease from Bacillus subtilis DC27 exhibits the highest amidolytic activity, with a hydrolysis rate of 225.291 mmol·min⁻¹·mL⁻¹^[1].
D-Val-Leu-Lys-pNA (0.16 mM) is hydrolyzed by Lys-gingipain from Porphyromonas gingivalis (strain H66) with a K_m of 0.2 mM, V_max of 133 mmol/min, and V_max/K_m ratio of 665^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

478.59

Formula

C₂₃H₃₈N₆O₅

CAS No.

63589-93-5

Appearance

Solid

Color

White to light yellow

SMILES

CC(C)[C@@H](N)C(N[C@@H](CC(C)C)C(N[C@@H](CCCCN)C(NC1=CC=C(C=C1)[N+]([O-])=O)=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (104.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0895 mL	10.4474 mL	20.8947 mL
5 mM	0.4179 mL	2.0895 mL	4.1789 mL
10 mM	0.2089 mL	1.0447 mL	2.0895 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.9%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (248 KB)

Handling Instructions (2659 KB)

References

[1]. Hu Y, et al. Purification and characterization of a novel, highly potent fibrinolytic enzyme from Bacillus subtilis DC27 screened from Douchi, a traditional Chinese fermented soybean food. Sci Rep. 2019;9(1):9235. Published 2019 Jun 25.

[2]. Pike R, et al. Lysine- and arginine-specific proteinases from Porphyromonas gingivalis. Isolation, characterization, and evidence for the existence of complexes with hemagglutinins. J Biol Chem. 1994 Jan 7;269(1):406-11.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0895 mL	10.4474 mL	20.8947 mL	52.2368 mL
	5 mM	0.4179 mL	2.0895 mL	4.1789 mL	10.4474 mL
	10 mM	0.2089 mL	1.0447 mL	2.0895 mL	5.2237 mL
	15 mM	0.1393 mL	0.6965 mL	1.3930 mL	3.4825 mL
	20 mM	0.1045 mL	0.5224 mL	1.0447 mL	2.6118 mL
	25 mM	0.0836 mL	0.4179 mL	0.8358 mL	2.0895 mL
	30 mM	0.0696 mL	0.3482 mL	0.6965 mL	1.7412 mL
	40 mM	0.0522 mL	0.2612 mL	0.5224 mL	1.3059 mL
	50 mM	0.0418 mL	0.2089 mL	0.4179 mL	1.0447 mL
	60 mM	0.0348 mL	0.1741 mL	0.3482 mL	0.8706 mL
	80 mM	0.0261 mL	0.1306 mL	0.2612 mL	0.6530 mL
	100 mM	0.0209 mL	0.1045 mL	0.2089 mL	0.5224 mL

D-Val-Leu-Lys-pNA Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D-Val-Leu-Lys-pNA63589-93-5S-2251S2251S 2251Fluorescent DyeBacillus subtilis DC27Lys-gingipainPorphyromonas gingivalis strain H66Porphyromonas gingivalisthrombosisperiodontal diseaseplasminplasminogenDFE27 serine proteaseInhibitorinhibitorinhibit

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D-Val-Leu-Lys-pNA (Synonyms: S-2251)

Other Forms of D-Val-Leu-Lys-pNA:

D-Val-Leu-Lys-pNA Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

D-Val-Leu-Lys-pNA Related Antibodies

Molarity Calculator

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