D-Val-Leu-Lys-pNA dihydrochloride
Based on 1 Customer Validation
D-Val-Leu-Lys-pNA (D-Val-Leu-Lys-p-nitroanilide) dihydrochloride is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA dihydrochloride acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA dihydrochloride binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity.
For research use only. We do not sell to patients.
- Purity : 99.92%
- CAS No.: 62354-43-2
- Formula: C23H40Cl2N6O5
- Molecular Weight:551.51
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Storage:
Stored under nitrogen, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
D-Val-Leu-Lys-pNA dihydrochloride (1 mM; 30 min at 37°C) was the substrate against which purified DFE27 serine protease from Bacillus subtilis DC27 exhibits the highest amidolytic activity, with a hydrolysis rate of 225.291 mmol·min−1·mL−1[1].
D-Val-Leu-Lys-pNA dihydrochloride (0.16 mM) is hydrolyzed by Lys-gingipain from Porphyromonas gingivalis (strain H66) with a Km of 0.2 mM, Vmax of 133 mmol/min, and Vmax/Km ratio of 665[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 62354-43-2
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Appearance Solid
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Molecular Weight 551.51
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Formula C23H40Cl2N6O5
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Color White to off-white
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Synonyms
D-Val-Leu-Lys-p-itoailide dihydrochloride; D-VLK-pNA dihydrochloride; S 2251 dihydrochloride
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Sequence
{d-Val}-Leu-Lys-{pNA}
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Sequence Shortening
{d-Val}-LK-{pNA}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Stored under nitrogen, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (181.32 mM; Need ultrasonic)
DMSO : 50 mg/mL (90.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
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Handling Instructions (2659 KB)
References
[1]. Hu Y, et al. Purification and characterization of a novel, highly potent fibrinolytic enzyme from Bacillus subtilis DC27 screened from Douchi, a traditional Chinese fermented soybean food. Sci Rep. 2019;9(1):9235. Published 2019 Jun 25. [Content Brief]
[2]. Pike R, et al. Lysine- and arginine-specific proteinases from Porphyromonas gingivalis. Isolation, characterization, and evidence for the existence of complexes with hemagglutinins. J Biol Chem. 1994 Jan 7;269(1):406-11. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.8132 mL | 9.0660 mL | 18.1320 mL | 45.3301 mL |
| 5 mM | 0.3626 mL | 1.8132 mL | 3.6264 mL | 9.0660 mL | |
| 10 mM | 0.1813 mL | 0.9066 mL | 1.8132 mL | 4.5330 mL | |
| 15 mM | 0.1209 mL | 0.6044 mL | 1.2088 mL | 3.0220 mL | |
| 20 mM | 0.0907 mL | 0.4533 mL | 0.9066 mL | 2.2665 mL | |
| 25 mM | 0.0725 mL | 0.3626 mL | 0.7253 mL | 1.8132 mL | |
| 30 mM | 0.0604 mL | 0.3022 mL | 0.6044 mL | 1.5110 mL | |
| 40 mM | 0.0453 mL | 0.2267 mL | 0.4533 mL | 1.1333 mL | |
| 50 mM | 0.0363 mL | 0.1813 mL | 0.3626 mL | 0.9066 mL | |
| 60 mM | 0.0302 mL | 0.1511 mL | 0.3022 mL | 0.7555 mL | |
| 80 mM | 0.0227 mL | 0.1133 mL | 0.2267 mL | 0.5666 mL | |
| H2O | 100 mM | 0.0181 mL | 0.0907 mL | 0.1813 mL | 0.4533 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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