T-PA Protein, Human (HEK293, His)
Based on 5 publication(s) in Google Scholar
T-PA is a key proteolytic enzyme that coordinates the conversion of the abundant but inactive zymogen plasminogen to its active form plasmin via hydrolysis of a single Arg-Val bond. This enzymatic conversion helps regulate plasmin-mediated proteolysis, a key process in tissue remodeling, degradation, and various pathophysiological events. T-PA Protein, Human (HEK293, His) is the recombinant human-derived T-PA protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
T-PA is a key proteolytic enzyme that coordinates the conversion of the abundant but inactive zymogen plasminogen to its active form plasmin via hydrolysis of a single Arg-Val bond. This enzymatic conversion helps regulate plasmin-mediated proteolysis, a key process in tissue remodeling, degradation, and various pathophysiological events. T-PA Protein, Human (HEK293, His) is the recombinant human-derived T-PA protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Tissue plasminogen activator (t-PA) is a pivotal protein that catalyzes the conversion of the abundant, yet inactive, zymogen plasminogen into the active protease plasmin, achieved by hydrolyzing a single Arg-Val bond in plasminogen. This enzymatic process is integral in controlling plasmin-mediated proteolysis, thereby playing a crucial role in tissue remodeling, degradation, and cell migration, as well as influencing various physiopathological events. Furthermore, t-PA exhibits functional significance during oocyte activation, participating in the cortical granule reaction in the zona reaction, which contributes to the prevention of polyspermy and serves as a protective mechanism during fertilization (By similarity). The multifaceted roles of t-PA underscore its importance in both physiological and reproductive processes, emphasizing its regulatory functions in proteolysis and cellular events.
In Vitro
T-PA Protein, Human (2.5-20 μg/mL, 48 h) enhances the adhesion and migration of T cells to bEnd.3 cells, upregulates the expression of ICAM-1 on the surface and in bEnd.3 cells[1].
In Vivo
T-PA Protein, Human (250 μg/kg, iv, once daily for 7 days) causes the early onset of experimental autoimmune encephalomyelitis (EAE) and increased disease severity in mouse MOG 35-55 (HY-P1240)-induced EAE model[1].
Verified Bioactivity
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is >500.47 pmol/min/µg, as measured under the described conditions.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Publications (5)
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Journal Impact Factor
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Most Recent
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Cell Rep Med
High-fat diet increases circulating palmitic acid produced by gut Bacteroides thetaiotaomicron to promote thrombosis. [Abstract]2025 Aug 19;6(8):102260. PMID: 40749683 -
Int J Biol Macromol
Exosomes enriched with miR-31-3p from keratinocytes under oxidative stress promote vitiligo progression by destructing melanocytes and activating CD8+ T cells. [Abstract]2025 May;310(Pt 1):143070. PMID: 40220810 -
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Nat Prod Bioprospect
2023 Nov 8;13(1):48. PMID: 37938456 -
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P00750-1 (S36-P562)
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Molecular Construction
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N-term
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T-PA (S36-P562)
Accession # P00750-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
PLAT; T-PA; Plasminogen Activator, Tissue Type; TPA; Tissue-Type Plasminogen Activator; Plasminogen Activator, Tissue; T-Plasminogen Activator; Plasminogen/Activator Kringle; Alteplase; Plasminogen Activator; Reteplase
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AA Sequence
SYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP
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Molecular Weight
Approximately 55-80 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 20 mM succinate, 4% trehalose, 4% mannitol, 0.2 mM CaCl2, 0.02% Tween 80, pH 4.5.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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