T-PA Protein, Human (HEK293, His)

T-PA is a key proteolytic enzyme that coordinates the conversion of the abundant but inactive zymogen plasminogen to its active form plasmin via hydrolysis of a single Arg-Val bond. This enzymatic conversion helps regulate plasmin-mediated proteolysis, a key process in tissue remodeling, degradation, and various pathophysiological events. T-PA Protein, Human (HEK293, His) is the recombinant human-derived T-PA protein, expressed by HEK293 , with C-6*His labeled tag.

Tissue plasminogen activator (t-PA) is a pivotal protein that catalyzes the conversion of the abundant, yet inactive, zymogen plasminogen into the active protease plasmin, achieved by hydrolyzing a single Arg-Val bond in plasminogen. This enzymatic process is integral in controlling plasmin-mediated proteolysis, thereby playing a crucial role in tissue remodeling, degradation, and cell migration, as well as influencing various physiopathological events. Furthermore, t-PA exhibits functional significance during oocyte activation, participating in the cortical granule reaction in the zona reaction, which contributes to the prevention of polyspermy and serves as a protective mechanism during fertilization (By similarity). The multifaceted roles of t-PA underscore its importance in both physiological and reproductive processes, emphasizing its regulatory functions in proteolysis and cellular events.

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is >500.47 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P00750-1 (S36-P562)

5327  [NCBI]

SYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP

Approximately 60-75 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Succinate, 4% Trehalose, 4% Mannitol, 0.2 mM CaCl₂,0.02% Tween 80, pH 4.5 or 20 mM MES, 150 mM NaCl, pH 5.5 or 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.