T-PA Protein, Human (HEK293, His)

T-PA is a key proteolytic enzyme that coordinates the conversion of the abundant but inactive zymogen plasminogen to its active form plasmin via hydrolysis of a single Arg-Val bond. This enzymatic conversion helps regulate plasmin-mediated proteolysis, a key process in tissue remodeling, degradation, and various pathophysiological events. T-PA Protein, Human (HEK293, His) is the recombinant human-derived T-PA protein, expressed by HEK293 , with C-6*His labeled tag.

Tissue plasminogen activator (t-PA) is a pivotal protein that catalyzes the conversion of the abundant, yet inactive, zymogen plasminogen into the active protease plasmin, achieved by hydrolyzing a single Arg-Val bond in plasminogen. This enzymatic process is integral in controlling plasmin-mediated proteolysis, thereby playing a crucial role in tissue remodeling, degradation, and cell migration, as well as influencing various physiopathological events. Furthermore, t-PA exhibits functional significance during oocyte activation, participating in the cortical granule reaction in the zona reaction, which contributes to the prevention of polyspermy and serves as a protective mechanism during fertilization (By similarity). The multifaceted roles of t-PA underscore its importance in both physiological and reproductive processes, emphasizing its regulatory functions in proteolysis and cellular events.

T-PA Protein, Human (2.5-20 μg/mL, 48 h) enhances the adhesion and migration of T cells to bEnd.3 cells, upregulates the expression of ICAM-1 on the surface and in bEnd.3 cells^[1].

T-PA Protein, Human (250 μg/kg, iv, once daily for 7 days) causes the early onset of experimental autoimmune encephalomyelitis (EAE) and increased disease severity in mouse MOG 35-55 (HY-P1240)-induced EAE model^[1].

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is >500.47 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P00750-1 (S36-P562)

5327  [NCBI]

Full Length of Isoform-1 Mature Protein

SYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP

Approximately 60-75 kDa due to the glycosylation

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM succinate, 4% trehalose, 4% mannitol, 0.2 mM CaCl₂, 0.02% Tween 80, pH 4.5.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wang J, et al., t-PA acts as a cytokine to regulate lymphocyte-endothelium adhesion in experimental autoimmune encephalomyelitis. Clin Immunol. 2014 May-Jun;152(1-2):90-100.  [Content Brief]