2. Others
  3. Fluorescent Dye
  4. Ac-IEPD-AMC

Ac-IEPD-AMC is a fluorogenic substrate for the determination of protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-IEPD-AMC

Ac-IEPD-AMC Chemical Structure

CAS No. : 216757-33-4

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Ac-IEPD-AMC:

Ac-IEPD-AMC Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ac-IEPD-AMC is a fluorogenic substrate for the determination of protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal[1].

Molecular Weight

671.69

Formula

C32H41N5O11
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Ac-Ile-Glu-Pro-Asp-{AMC}
Sequence Shortening

Ac-IEPD-{AMC}
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (148.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4888 mL 7.4439 mL 14.8878 mL
5 mM 0.2978 mL 1.4888 mL 2.9776 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4888 mL 7.4439 mL 14.8878 mL 37.2196 mL
5 mM 0.2978 mL 1.4888 mL 2.9776 mL 7.4439 mL
10 mM 0.1489 mL 0.7444 mL 1.4888 mL 3.7220 mL
15 mM 0.0993 mL 0.4963 mL 0.9925 mL 2.4813 mL
20 mM 0.0744 mL 0.3722 mL 0.7444 mL 1.8610 mL
25 mM 0.0596 mL 0.2978 mL 0.5955 mL 1.4888 mL
30 mM 0.0496 mL 0.2481 mL 0.4963 mL 1.2407 mL
40 mM 0.0372 mL 0.1861 mL 0.3722 mL 0.9305 mL
50 mM 0.0298 mL 0.1489 mL 0.2978 mL 0.7444 mL
60 mM 0.0248 mL 0.1241 mL 0.2481 mL 0.6203 mL
80 mM 0.0186 mL 0.0930 mL 0.1861 mL 0.4652 mL
100 mM 0.0149 mL 0.0744 mL 0.1489 mL 0.3722 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Ac-IEPD-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-IEPD-AMC216757-33-4Fluorescent Dyefluorogenic substratehydrolysis7-amino-4-methylcoumarinfluorescentInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ac-IEPD-AMC
Cat. No.:
HY-P4417
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.